Highly catalytically active pet hydrolase mutants

A mutant, hydrolase technology, applied in hydrolase, biochemical equipment and methods, fermentation and other directions, can solve the problems of low efficiency of PET plastic degradation, insufficient catalytic function of key enzyme Ec_PETase, difficult industrial application, etc. Degradation cost, high enzymatic activity, effect of simplifying degradation steps
CN107674866BActive Publication Date: 2020-09-18UNIV OF ELECTRONICS SCI & TECH OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
UNIV OF ELECTRONICS SCI & TECH OF CHINA
Publication Date
2020-09-18

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Abstract

The invention belongs to the field of protein engineering, in particular to a PET hydrolase mutant. The technical problem to be solved by the present invention is that the activity of the PET hydrolase (PETase) currently derived from the Ideonella sakaiensis 201-F6 strain is not ideal. The technical solution of the present invention to solve the technical problem is to provide a PET hydrolase mutant. In the present invention, 5 mutation hotspots are obtained through a large number of researches on PET hydrolase (PETase); 14 mutants are constructed by applying site-directed mutation technology, and the activity of 2 mutant ETase enzymes is finally screened out compared with the wild type Ec_PETase. , has a good application prospect.
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Description

technical field

[0001] The invention belongs to the technical field of Escherichia coli genetic engineering, in particular to a PET hydrolase (Ec_PETase) mutant derived from Escherichia coli engineering bacteria with improved catalytic activity. Background technique

[0002] Polyethylene terephthalate (PET) is one of the most common plastic materials. In 2013, the annual output of PET reached 56 million tons, accounting for about 1 / 5 of the global plastic output. According to statistics, PET is currently the most recycled plastic material, and its recycling rates in the United States and the European Union are 31% and 50% respectively. Although the PET polymer is only composed of two simple monomers connected by an ester bond, the ester bond is relatively strong and difficult to degrade naturally. Tens of millions of tons of PET plastic waste are difficult to be effectively disposed of every year, which is extremely harmful to the environment.

[0003] Currently existing PE...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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