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Method for removing proinflammatory factor in autologous conditioned serum (ACS)

A technology of cytokines and pro-inflammatory factors, which is applied in the field of biomedicine, can solve the problem of increased side effects in the hyaluronic acid treatment group, and achieve the effect of less side effects and good curative effect

Inactive Publication Date: 2018-02-23
YUNNAN SHUNXI REGENERATION MEDICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Frisbie et al. proved that autologous serum technology has a significant effect on the treatment of knee arthritis; Astolfi et al. proved the positive effect of autologous serum technology in the treatment of osteoarthritis; Baltzer et al. proved that autologous serum technology has a positive clinical effect on hip arthritis; Baltzer et al. etc. have proved that autologous serum has a very good effect on osteoarthritis, and in terms of the incidence of side effects, there is no significant difference between the autologous serum treatment group and the placebo group, but the side effects of the hyaluronic acid treatment group are significantly increased

Method used

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  • Method for removing proinflammatory factor in autologous conditioned serum (ACS)
  • Method for removing proinflammatory factor in autologous conditioned serum (ACS)
  • Method for removing proinflammatory factor in autologous conditioned serum (ACS)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for removing pro-inflammatory factors in cytokine-rich serum, comprising the steps of:

[0044] Step (1), preparation of blood collection equipment: Wash 3 mm medical grade glass beads (VWR International) with ultrapure water and dry them. Add 10 beads per 3 mL of whole blood and pre-place the glass beads in the direct The blood sampler obtained from the serum rich in cytokines was then autoclaved, dried at 65°C, and set aside; wherein, the temperature of autoclaving was 121°C, the pressure was 100kPa, and the sterilization time was 20 minutes;

[0045] At the same time, prepare the antigen-antibody binding reaction reagent, the antigen-antibody binding reaction reagent is binding / cleaning Buffer, containing 0.15M NaCl and 20mM NaCl 2 HPO 4 , the pH is 7.0;

[0046] Step (2), the acquisition of cytokine-rich serum: After the venous blood is collected, shake it well and slowly to ensure that the glass beads are fully in contact with the fresh whole blood, and ...

Embodiment 2

[0057] A method for removing pro-inflammatory factors in cytokine-rich serum, comprising the steps of:

[0058] Step (1), preparation of blood collection equipment: Wash 3 mm medical grade glass beads (VWR International) with ultrapure water and dry them. Add 10 beads per 3 mL of whole blood and pre-place the glass beads in the direct The blood sampler obtained from the serum rich in cytokines was then autoclaved, dried at 70°C, and set aside; wherein, the temperature of autoclaving was 121°C, the pressure was 100kPa, and the sterilization time was 20 minutes;

[0059] At the same time, prepare the antigen-antibody binding reaction reagent, the antigen-antibody binding reaction reagent is binding / cleaning Buffer, containing 0.15M NaCl and 20mM NaCl 2 HPO 4 , the pH is 7.0;

[0060] Step (2), the acquisition of cytokine-rich serum: After the venous blood is collected, shake it well and slowly to ensure that the glass beads are fully in contact with the fresh whole blood, and ...

Embodiment 3

[0071] A method for removing pro-inflammatory factors in cytokine-rich serum, comprising the steps of:

[0072] Step (1), preparation of blood collection equipment: Wash 3 mm medical grade glass beads (VWR International) with ultrapure water and dry them. Add 10 beads per 3 mL of whole blood and pre-place the glass beads in the direct The blood sampler obtained from the serum rich in cytokines was then autoclaved, dried at 68°C, and set aside; wherein, the temperature of autoclaving was 121°C, the pressure was 100kPa, and the sterilization time was 20 minutes;

[0073] At the same time, prepare the antigen-antibody binding reaction reagent, the antigen-antibody binding reaction reagent is binding / cleaning Buffer, containing 0.15M NaCl and 20mM NaCl 2 HPO 4 , the pH is 7.0;

[0074]Step (2), the acquisition of cytokine-rich serum: After the venous blood is collected, shake it well and slowly to ensure that the glass beads are fully in contact with the fresh whole blood, and p...

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Abstract

The invention relates to a method for removing proinflammatory factor from autologous conditioned serum (ACS), and belongs to the technical field of biological medicine. According to the method, the proinflammatory factor in ACS is removed by human peripheral blood collection, ACS acquisition and antigen-antibody binding technology, and related detection is carried out on ACS without proinflammatory factor, so that the removing specificity is ensured. The method can be used for removing useless proinflammatory factor ingredients from ACS, and accords with clinical application standard after detection. The ACS obtained by the method shows better curative effect and less side effect in clinical application.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for removing proinflammatory factors in serum rich in cytokines. Background technique [0002] Osteoarthritis is a degenerative disease, which is caused by aging, obesity, strain, trauma, congenital abnormalities of joints, joint deformities and many other factors. Clinical manifestations include slowly developing joint pain, tenderness, stiffness, joint swelling, limited mobility, and joint deformity. [0003] Conventional treatment of osteoarthritis is divided into conservative treatment and surgical treatment. Conservative treatment is mainly based on systemic medication and intra-articular topical medication. For patients with early osteoarthritis, oral anti-inflammatory and analgesic drugs or drugs for promoting blood circulation and removing blood stasis can be given symptomatically. Intra-articular injection of articular cartilage protective agen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/16A61P19/08A61P29/00A61M1/02
Inventor 陈曦李一佳
Owner YUNNAN SHUNXI REGENERATION MEDICAL ENG CO LTD