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Preparation method of glycyrrhizia polysaccharide with anti-aging activity

A licorice polysaccharide, anti-aging technology, applied in the field of preparation of licorice polysaccharide, can solve the problems of low polysaccharide activity, high production cost, and inability to industrialize production

Active Publication Date: 2018-02-23
青岛新昆仑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] To sum up, the current methods to obtain active licorice polysaccharides have disadvantages such as long time-consuming, high production costs, cumbersome steps, low activity of the obtained polysaccharides, and inability to industrialized production.

Method used

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  • Preparation method of glycyrrhizia polysaccharide with anti-aging activity

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preparation example Construction

[0027] The invention provides a preparation method of licorice polysaccharide with anti-aging activity, comprising the following steps:

[0028] A) mixing and heating the licorice raw material and No. 1 solvent, and filtering to obtain crude licorice polysaccharide;

[0029] The No. 1 solvent is selected from one or more of phosphoric acid aqueous solution, acetic acid aqueous solution, hydrochloric acid aqueous solution, sulfuric acid aqueous solution, methanol, ethanol, isopropanol and acetone;

[0030] B) mixing the crude licorice polysaccharide with No. 2 solvent to obtain a mixed solution, adding No. 3 solvent to the mixed solution to mix, standing for stratification, and obtaining a supernatant;

[0031] The No. 2 solvent is selected from water, aqueous ammonia solution, NaCO 3 aqueous solution, NaOH aqueous solution or KOH aqueous solution.

[0032] The No. 3 solvent is selected from the mixed solvent of methylene chloride or methylene chloride and n-butanol;

[0033...

Embodiment 1

[0061] Embodiment 1 prepares licorice polysaccharide

[0062] (1) Separation: Weigh 300g of licorice cream, add 15 times the amount (V / M) (ml / g) of 1% hydrochloric acid ethanol solution, reflux for 1 hour, and filter to obtain insoluble matter, which is the crude licorice polysaccharide;

[0063] (2) Purification: Get the crude product of glycyrrhiza polysaccharide obtained in step (1), add 15 times of amount (V / M) (ml / g) 2% NaOH aqueous solution to dissolve and obtain No. 1 solution, add 6 times to No. 1 solution Volumetric dichloromethane and mixed for 30 minutes, standing for 1 hour, collecting the upper layer to obtain the No. In the amount (V / V) of methanol, an off-white solid was precipitated, which was glycyrrhizic polysaccharide, which was filtered off and dried, and the content of glycyrrhizic polysaccharide was 82.47%.

Embodiment 2

[0070] Embodiment 2 prepares licorice polysaccharide

[0071] (1) Separation: Weigh 300 g of licorice extract powder, add 20 times the amount (V / M) (ml / g) of 1% ethanol hydrochloride, reflux for 1 hour, and filter to obtain insoluble matter, which is the crude licorice polysaccharide;

[0072] (2) Purification: Get the obtained licorice polysaccharide crude product in step (1), add 20 times of amount (V / M) (ml / g) 2% ammonia water solution after dissolving to obtain No. 1 solution, add 3 times to No. 1 solution volume of dichloromethane and mixed for 30 minutes, let the layers stand for 1.5 hours, collected the upper layer to obtain the No. In the double amount (V / V) of ethanol, an off-white solid was precipitated, which was glycyrrhizic polysaccharide, which was filtered out and dried, and the content of glycyrrhizic polysaccharide was 88.47%.

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Abstract

The invention provides a preparation method of glycyrrhizia polysaccharide. The glycyrrhizia polysaccharide with higher anti-aging activity can be obtained by selecting specific solvents and adoptinga simple solvent-mixing step. The preparation method is simple.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a preparation method of licorice polysaccharide with anti-aging activity. Background technique [0002] Glycyrrhiza polysaccharide is one of the main active ingredients of licorice. After Zhou Rong et al. separated and purified the licorice polysaccharide, the high-performance capillary electrophoresis analysis of the sample showed that the polysaccharide component was composed of rhamnose, dextran, arabinose and galactose, and Dextran as the main chain. Takada K. Controlled Smith degradation and limited hydrolysis of licorice polysaccharides isolated from Northwestern licorice stolons. Methylation analysis of the first and second Smith degradation products and limited hydrolyzate indicated that the core structure of Glycyrrhizae polysaccharide contains a backbone composed of β-1,3-linked D-galactose residues. The 3 / 5 galactose unit of the main ch...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 骆俊才高艳艳张浩忠于晓丽李宗龙
Owner 青岛新昆仑生物科技有限公司
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