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Grape pip fermented raw stock cosmetic as well as preparation method and application thereof

A technology for fermenting puree and grape seeds, applied in the biological field, can solve the problems of low extraction rate, low safety, long time consumption, etc., and achieve the effects of simple operation, simplified production steps and scavenging of DPPH free radicals

Active Publication Date: 2017-05-31
SHANGHAI BIOTRULY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional extraction techniques include organic solvent extraction, hot water extraction, systemic solvent extraction, etc., but these methods have low product safety, long time consumption and low extraction rate

Method used

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  • Grape pip fermented raw stock cosmetic as well as preparation method and application thereof
  • Grape pip fermented raw stock cosmetic as well as preparation method and application thereof
  • Grape pip fermented raw stock cosmetic as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, preparation grape seed fermented protoplasm

[0043] Prepare grape seed fermented puree according to the following steps:

[0044] (1) The concentration obtained in 6mL step 3 is 10 5 ~10 8 Inoculate 22g of dried grape seed powder and 300mL of water with CFU / mL fermented bacteria liquid to obtain a fermentation system;

[0045] (2) Fermenting the above fermentation system in a shaker at 45° C. for 36 hours to obtain a fermentation product;

[0046] (3) Sterilize the above-mentioned fermentation product at 115°C for 30 minutes to inactivate the bacteria to obtain the sterilized fermentation product; centrifuge the sterilized fermentation product at 4000 r / min and a centrifugal radius of 9 cm for 10 minutes, discard Precipitate, collect the supernatant, which is the grape seed fermentation puree.

Embodiment 2

[0047] Embodiment 2, the application of grape seed fermented puree as cosmetics

[0048] 1. Properties of grape seed fermented puree

[0049] The fermented puree prepared in Example 1 was decolorized and deodorized by activated carbon. Its appearance is a viscous liquid, and its color is light yellow to brownish yellow. The pH value is 6.2-7.0, the viscosity is 190-230cP, the soluble solid content is 3.0-3.5%, the total number of colonies is less than 50CFU / mL, and no pathogenic bacteria are detected. According to the hygienic standard GB7916-87 for cosmetics, the total number of bacteria in cosmetics is not higher than 1000CFU / ml, so this fermented puree meets the quality requirements of cosmetics.

[0050] The composition analysis of the grape seed fermentation puree is carried out. The detection method of crude polysaccharides refers to GB / T 5009.8-2008; the detection method of flavonoids refers to GB / T 5009.124-2003; T 22244-2008, the results obtained are as follows:

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Abstract

The invention provides a grape pip fermented raw stock cosmetic as well as a preparation method and application thereof. The preparation method of the grape pip fermented raw stock cosmetic comprises the following steps: mixing grape pip dry powder, water and a bacterial solution to obtain an initial system, and carrying out fermental cultivation on the initial system so as to obtain a grape pip fermented raw stock. In the bacterial solution, the culture is lactococcus lactis, specifically can be lactobacillus. The application of the grape pip fermented raw stock in the preparation of the cosmetic with at least one function of the following functions 1) to 4) or the application of the grape pip fermented raw stock directly serving as the cosmetic with at least one function of the following functions 1) to 4) is also in the protective range of the invention: 1) eliminating DPPH free radicals; 2) stimulating collagen generation; 3) resisting oxidation; and 4) resisting aging. The cosmetic is facial mask, essence or toner. According to the method, active substances are extracted from grape pips by using a fermentation engineering technology, the operation is simple, and the prepared grape pip fermented substance, namely the grape pip fermented raw stock, is safe and harmless for the skin due to the fact that no foreign matter is added, so that the cosmetic has higher anti-aging activity.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a grape seed fermented puree cosmetic and its preparation method and application. Background technique [0002] Various components in grape seeds have extremely high nutritional value and health value. Grape seeds are rich in water protein, amino acid, minerals, fat, carbohydrates and dietary fiber and other nutrients. In addition, it also contains a variety of functional ingredients, including proanthocyanidins, grape seed oil, resveratrol, vitamin E, tannin, etc., all of which have high nutritional value and beauty and health care effects. The polyphenolic natural antioxidants in grape seeds are much richer in content and type than grape skin and grape pulp, and these polyphenolic substances in the seeds show a strong antioxidant capacity. Fermenting and applying grape seeds can give full play to the cosmetic effect of functional components in grape seeds, and the bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/08
CPCA61K8/97A61K2800/85A61Q19/08
Inventor 王昌涛李萌方祥铭方晓薇赵丹张佳婵许丹妮
Owner SHANGHAI BIOTRULY BIOTECH CO LTD
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