Novel nucleic acid detection microfluidic chip device

Abstract

The invention relates to a novel nucleic acid detection microfluidic chip device for automatic nucleic acid extraction, purification and detection. The novel nucleic acid detection microfluidic chip device has a sealed chamber structure, which includes a waste liquid chamber, a sample reagent chamber, a mixing chamber, a pre-amplification chamber, an amplification chamber, a biosensor and a signal output port, a waste liquid chamber, a sample reagent The chamber, mixing chamber, pre-amplification chamber, and amplification chamber are connected by fluid microchannels, and the biosensor and signal output port are connected by electrical signals. The fluid microchannel is in the shape of a three-dimensional tube in the novel nucleic acid detection microfluidic chip device distribution; the waste liquid chamber, the sample reagent chamber, the mixing chamber, and the pre-amplification chamber are respectively equipped with micropumps, or the waste liquid chamber, the sample reagent chamber, the mixing chamber, and the pre-amplification chamber are respectively equipped with external micropumps. the split hole. The invention uses the micropump to clean the pipeline, which can realize cyclic and continuous detection, realize one or more samples, one or multiple detections, and reduce the use cost.

Description

technical field [0001] The invention relates to a nucleic acid detection device, in particular to a novel nucleic acid detection microfluidic chip device for automatic nucleic acid extraction, purification and detection. Background technique [0002] Traditional nucleic acid detection methods include nucleic acid extraction, separation, and purification; the purified nucleic acid is further tested for genetic information such as PCR. Traditional nucleic acid detection is complex and has cumbersome steps. It needs to be operated in a specialized laboratory, and operators must pass professional training, which limits the promotion of nucleic acid detection. At the same time, traditional nucleic acid testing cannot detect multiple components at one time. If multiple items need to be tested, multiple parallel tests must be performed. In addition, since traditional nucleic acid detection is an open experimental operation, there are certain cross-contamination problems in manual ...

AI Technical Summary

Problems solved by technology

The existing microfluidic chip is only a single functional unit for amplification, elution, separation, etc.

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