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A kind of chlorinated algae organic matter and its preparation method and toxicity analysis method

An analysis method and organic matter technology, applied in the detection field, can solve the problems of inability to reflect the toxicity of the human body, low accuracy of results, cumbersome operation, etc., and achieve high accuracy, low detection limit, and good sensitivity

Active Publication Date: 2021-06-11
GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, animal P450 enzymes differ greatly from their mammalian counterparts in substrate specificity, which does not reflect the human toxicity profile of the substance
In addition, the operation of EROD experiment is cumbersome, the accuracy of the result is not high, and there are certain differences between different experiments.
The human cell model is an ideal model for simulating the human body and detecting toxicity, but how to select the detection target is the difficulty in applying the cell model to the toxicity analysis of Cl-AOM

Method used

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  • A kind of chlorinated algae organic matter and its preparation method and toxicity analysis method
  • A kind of chlorinated algae organic matter and its preparation method and toxicity analysis method

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Embodiment 1

[0033] Embodiment 1 A kind of preparation method of chlorinated algae organic matter solution

[0034] A kind of preparation method of chlorinated algae organic matter solution of the present embodiment, comprises the following steps:

[0035] (1), use BG11 medium to cultivate Chlorella, cultivate under 1000 lux (lux) light, and the light cycle is 14:10h. When the algae grows to the logarithmic phase, centrifuge at 5000 rpm for 10 minutes, and collect the small algae. Chlorella, washed once with ultrapure water, freeze-dried at low temperature, and ground with liquid nitrogen to obtain chlorella algae powder;

[0036] (2) After dissolving in ultrapure water, pass through a 0.45 μm filter membrane, use a TOC instrument to measure the total organic carbon content, and use pH 7.0 disodium hydrogen phosphate-citric acid buffer to dissolve the algae powder again to adjust the total organic carbon The content is 40mg / L, centrifuged at 8000rpm for 5min, and the supernatant is passed...

Embodiment 2

[0038] Embodiment 2 A kind of preparation method of chlorinated algae organic matter solution

[0039] A kind of preparation method of chlorinated algae organic matter solution of the present embodiment, comprises the following steps:

[0040] (1), use BG11 medium to cultivate Chlorella, cultivate under 1000 lux (lux) light, and the light cycle is 14:10h. When the algae grows to the logarithmic phase, centrifuge at 5000 rpm for 10 minutes, and collect the small algae. Chlorella, washed once with ultra-pure water, dried in vacuum, ground with liquid nitrogen to obtain chlorella algae powder;

[0041] (2), after using ultrapure water to dissolve, pass through a 0.45 μm filter membrane, use a TOC instrument to measure the total organic carbon content, use the glycine-sodium hydroxide buffer solution of pH 7.0 to dissolve the algae powder again, and adjust the total organic carbon content to 60mg / L, centrifuge at 8000rpm for 5min, take the supernatant and pass through a 0.45um filt...

Embodiment 3

[0043] Embodiment 3 A kind of preparation method of chlorinated algae organic matter solution

[0044] A kind of preparation method of chlorinated algae organic matter solution of the present embodiment, comprises the following steps:

[0045] (1), use BG11 medium to cultivate Chlorella, cultivate under 1000 lux (lux) light, and the light cycle is 14:10h. When the algae grows to the logarithmic phase, centrifuge at 5000 rpm for 10 minutes, and collect the small algae. For algae, wash once with ultrapure water, dry with nitrogen, and grind with liquid nitrogen to obtain chlorella algae powder; drying with liquid nitrogen requires less equipment, and the drying effect is good, which can reduce costs and improve efficiency.

[0046] (2) After dissolving in ultrapure water, pass through a 0.45 μm filter membrane, use a TOC instrument to measure the total organic carbon content, use pH 7.0 phosphate buffer to dissolve the algae powder again, and adjust the total organic carbon cont...

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Abstract

The invention discloses a chlorinated algae organic matter solution, a preparation method and a toxicity analysis method thereof. The chlorinated algae organic matter solution of the present invention obtains the chlorella algae body by first adopting the method of blowing dry with nitrogen gas, redissolves after grinding with liquid nitrogen, keeps the total organic carbon content of the solution at 40-60 mg / L, chlorinates, and dissolves with alkali Neutralize the neutralized solution, and prepare a stable Cl-AOM solution. The Cl-AOM toxicity analysis of the present invention is to detect the expression levels of CYP1A1 and CYP1B1 in the P450 system after Cl-AOM is exposed to caco-2 cells by PCR technology. Realized, it directly performs toxicity detection at the level of Cl‑AOM mixed solution, which greatly saves time. In addition, PCR technology can detect trace differences in gene expression, has the characteristics of low detection limit, high accuracy and good sensitivity, and can detect gene expression differences caused by trace toxic substances.

Description

technical field [0001] The invention belongs to the technical field of detection, and more specifically, the invention relates to a chlorinated algae organic matter, a preparation method thereof and a toxicity analysis method. Background technique [0002] The overgrowth of algae (chlorella) in the water body will lead to the increase of organic matter in the water body. After the organic matter is chlorinated and disinfected by the water plant, hundreds of disinfection by-products (Disinfectionbyproducts, DBPs), such as chloroform and chloroform, will be produced. and chloroacetonitrile etc. At present, there have been a large number of reports on the toxicity analysis of single DBPs, but there are few reports on the preparation and toxicity studies of chlorinated algae organic matter (Cl-AOM). Cl-AOM enters the human body through the digestive system, and its toxicity is It is the result of the comprehensive action of various DBPs and other toxic substances contained in i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P1/00C12Q1/686C12R1/89
CPCC12N1/12C12P1/00C12Q1/686C12N1/125C12R2001/89
Inventor 梁岩吴彬彬
Owner GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI