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Saccharose transporter ShSWEET1 gene and application thereof

A transporter and cane sugar technology, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as difficulty in increasing the total sugar content, failure to increase the sugar cane content as a whole, narrow genetic basis of production species, etc., to achieve improved The effect of plant resistance to cold stress

Active Publication Date: 2018-02-23
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are still many problems in the cultivation and management of sugarcane in China, and there is still a lot of room for improving the cultivation and management of sugarcane to increase the biological yield of sugarcane
At the same time, the biggest bottleneck faced by sugarcane planting in my country is the serious trend of simplification of varieties. In production, it is urgent to cultivate new varieties with high yield and high sugar content for production. Due to the large number of chromosomes and complex genome structure of sugarcane and the The narrow genetic basis of the cane species makes it take a long time for conventional breeding and it is difficult to increase the total sugar content. The genetic engineering method only changes the distribution of carbon substances, but does not increase the sugar content of sugarcane as a whole, so it is still far from meeting the needs of production. The needs of life (Zhao Tingting, 2012)

Method used

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  • Saccharose transporter ShSWEET1 gene and application thereof
  • Saccharose transporter ShSWEET1 gene and application thereof
  • Saccharose transporter ShSWEET1 gene and application thereof

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Embodiment Construction

[0033] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention. The experimental method that does not indicate specific experimental condition in the following examples, generally according to conventional conditions, molecular cloning (Molecular Cloning: A Laboratory Manual, 3rd ed.) or yeast genetic method experiment guideline (Methodsin Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual , Adams A et al edited, Cold Spring Harbor Laboratory, 1998 publication), or according to the conditions suggested by the manufacturer.

[0034] 1 Experimental materials

[0035] 1.1 Plant material

[0036]Sugarcane Xintaitang No. 22 (ROC22) planted in the Lingao Experimental Base of the Institute of Tropical Biotechnology of the Chinese Academy of Tropical Agricultural Sciences and the Haikou Campus of the Chinese Academy of Tropical Agricultural Sciences wa...

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Abstract

The invention provides a saccharose transporter ShSWEET1 gene. The nucleotide sequence thereof is shown as SEQ ID NO:1. The invention also provides a protein encoded with the ShSWEET1 gene and an application of the ShSWEET1 gene. According to the invention, the ShSWEET1 gene is firstly obtained by cloning from sugarcane; the gene has a transport function of a sugar transporter and can be located in the cytomembrane of the cell; the resistance of plants against chilling stress can be promoted; the growth of the plants, such as sugarcane under an adverse condition is benefited; a genetic basis is provided for revealing the regulating function of saccharose transport mechanism for the growth development and quality improvement of the plant and for researching the genetic engineering for increasing the crop yield and improving the quality.

Description

technical field [0001] The invention relates to the field of biological technology, and relates to a gene of sugarcane sugar transporter ShSWEET1 and its application. Background technique [0002] Sugarcane (Saccharum officinarum L.) belongs to Poaceae, Sorghum family, Saccharum subfamily, Saccharum genus, annual or perennial perennial herb, homologous with corn and sorghum, native to tropical and subtropical regions of New Guinea or India, sugarcane as C4 plants are among the most efficient at converting solar energy into chemical energy. Sugarcane plants have high light saturation point, strong carbon dioxide fixation capacity, high photosynthetic rate, high photosynthetic intensity, high biomass, rich sugar content and can also be used as biofuel. In the past 30 years, sugarcane has received global attention as a superior potential alternative renewable energy source. Carbon allocation is a key process of energy and material allocation in plant photosynthesis. In gener...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/10C12N15/11C12N15/82A01H6/46
CPCC07K14/415C12N15/10C12N15/11C12N15/8245C12N15/8273C12Q2531/113
Inventor 王俊刚赵婷婷张树珍王文治杨本鹏冯翠莲冯小艳曾军蔡文伟熊国如伍苏然
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI