Method for preparing instant black tea by utilizing microbial liquid fermentation
A liquid fermentation and microbial technology, which is applied in tea extraction and pre-extraction tea treatment, etc., can solve the problems of long fermentation period, extensive processing, and difficult process control.
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Embodiment 1
[0020] Sun-dried green tea with large leaves from Yunnan to remove non-tea impurities, homogenized and crushed, then soaked with water, the amount of water added is 1:8, steamed at high temperature to prepare fermentation material; , Penicillium and saccharomyces were co-inoculated in the liquid medium, and the shake flask was cultivated at 28°C for 60h, and the obtained seed liquid was inserted in a weight ratio of 5% of the fermentation material. The temperature of the fermentation process was 25°C, and the oxygen flow rate was 0.3 L / min, the stirring speed is 350rpm, the cycle is 8 days, and finally the fermented liquid is obtained; the fermented liquid is inactivated, centrifuged or filtered to obtain fermented tea juice, and then concentrated and dried to obtain instant black tea powder.
Embodiment 2
[0022] Sun-dried green tea with large leaves from Yunnan to remove non-tea impurities, homogenate and crush it, add water to infiltrate, the amount of water added is 1:10, and steam the material at high temperature to prepare fermentation material; , Penicillium and saccharomyces were co-inoculated in the liquid medium, and cultured in shake flasks at 24°C for 72h, and the obtained seed liquid was inserted in a weight ratio of 6.5% of the fermentation material. The temperature of the fermentation process was 30°C, and the oxygen flow rate was 2.5 L / min, the stirring speed is 250rpm, the cycle is 6 days, and finally the fermented liquid is obtained; the fermented liquid is inactivated, centrifuged or filtered to obtain fermented tea juice, and then concentrated and dried to obtain instant black tea powder.
Embodiment 3
[0024] Sun-dried green tea with large leaves from Yunnan to remove non-tea impurities, homogenate and crush it, add water to infiltrate, the amount of water added is 1:13, and steam the material at high temperature to prepare fermentation material; , Penicillium and saccharomyces were co-inoculated in the liquid medium, and cultured in shake flasks at 30°C for 48 hours, and the obtained seed liquid was inserted in a weight ratio of 3.5% of the fermentation material. The temperature of the fermentation process was 20°C, and the oxygen flow rate was 1.2 L / min, the stirring speed is 220rpm, the cycle is 10 days, and finally the fermented liquid is obtained; the fermented liquid is inactivated, centrifuged or filtered to obtain fermented tea juice, and then concentrated and dried to obtain instant black tea powder.
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