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Method for preparing instant black tea by utilizing microbial liquid fermentation

A liquid fermentation and microbial technology, which is applied in tea extraction and pre-extraction tea treatment, etc., can solve the problems of long fermentation period, extensive processing, and difficult process control.

Inactive Publication Date: 2018-03-09
申国庆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inoculate black tea dominant bacteria on tea leaves, and prepare traditional black tea by solid state fermentation. This method has problems such as extensive processing, long fermentation cycle, and difficult control of the process.

Method used

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  • Method for preparing instant black tea by utilizing microbial liquid fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Sun-dried green tea with large leaves from Yunnan to remove non-tea impurities, homogenized and crushed, then soaked with water, the amount of water added is 1:8, steamed at high temperature to prepare fermentation material; , Penicillium and saccharomyces were co-inoculated in the liquid medium, and the shake flask was cultivated at 28°C for 60h, and the obtained seed liquid was inserted in a weight ratio of 5% of the fermentation material. The temperature of the fermentation process was 25°C, and the oxygen flow rate was 0.3 L / min, the stirring speed is 350rpm, the cycle is 8 days, and finally the fermented liquid is obtained; the fermented liquid is inactivated, centrifuged or filtered to obtain fermented tea juice, and then concentrated and dried to obtain instant black tea powder.

Embodiment 2

[0022] Sun-dried green tea with large leaves from Yunnan to remove non-tea impurities, homogenate and crush it, add water to infiltrate, the amount of water added is 1:10, and steam the material at high temperature to prepare fermentation material; , Penicillium and saccharomyces were co-inoculated in the liquid medium, and cultured in shake flasks at 24°C for 72h, and the obtained seed liquid was inserted in a weight ratio of 6.5% of the fermentation material. The temperature of the fermentation process was 30°C, and the oxygen flow rate was 2.5 L / min, the stirring speed is 250rpm, the cycle is 6 days, and finally the fermented liquid is obtained; the fermented liquid is inactivated, centrifuged or filtered to obtain fermented tea juice, and then concentrated and dried to obtain instant black tea powder.

Embodiment 3

[0024] Sun-dried green tea with large leaves from Yunnan to remove non-tea impurities, homogenate and crush it, add water to infiltrate, the amount of water added is 1:13, and steam the material at high temperature to prepare fermentation material; , Penicillium and saccharomyces were co-inoculated in the liquid medium, and cultured in shake flasks at 30°C for 48 hours, and the obtained seed liquid was inserted in a weight ratio of 3.5% of the fermentation material. The temperature of the fermentation process was 20°C, and the oxygen flow rate was 1.2 L / min, the stirring speed is 220rpm, the cycle is 10 days, and finally the fermented liquid is obtained; the fermented liquid is inactivated, centrifuged or filtered to obtain fermented tea juice, and then concentrated and dried to obtain instant black tea powder.

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Abstract

The invention discloses a method for preparing instant black tea by utilizing microbial liquid fermentation. The method comprises the following steps: homogenizing Yunnan large-leaf tea subjected to sunshine withering, adding water to infiltrate, and steaming at high temperature to prepare a fermented material; taking eurotium cristatum, aspergillus niger, penicillium and saccharomycetes as a seedsolution, performing microbial liquid fermentation, inactivating, centrifuging or filtering the fermentation liquor to obtain fermented tea soup, and concentrating and drying, thereby obtaining the instant tea powder. The method disclosed by the invention is improved on the basis of the prior art, and due to reasonable mixing of different strains, the microbial fermentation ability is fully achieved, so that the fermented tea liquor is strong in aroma and stable in quality, and the method is simple to operate and suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of food and biological processing, and in particular relates to a method for preparing instant black tea by liquid-state fermentation of microorganisms. Background technique [0002] Instant tea is made from finished tea or fresh tea leaves, extracted with water or fresh tea leaves, filtered, concentrated, and dried. Food additives, food processing aids, and appropriate food accessories can be added during the production process. A convenient solid instant tea product in the form of powder or flakes or granules similar to instant coffee. In addition to the nutrients and flavor substances that can enter the tea soup in traditional tea, instant tea also has the advantages of being convenient to drink, easy to store and transport, and free of harmful substances such as pesticide residues and heavy metals. Therefore, it is currently a very promising drink in the world. [0003] Dark tea has various health effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/30A23F3/10
CPCA23F3/10A23F3/30
Inventor 申国庆
Owner 申国庆