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Drug for treating tumor

A tumor treatment and drug technology, applied in the direction of anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problems of limited cell sources and high production costs, and achieve the effects of high yield, prolonging activity, and reducing toxic effects

Pending Publication Date: 2018-03-16
HUBEI SOUNDNY BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Chinese patent application 201110241369.8 discloses a tumor chemotherapeutic drug preparation, which is made of apoptotic tumor cell vesicles wrapped with chemotherapeutic drugs. Although it has some advantages over other nano-drugs, there are still problems such as limited cell sources and high production costs.

Method used

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  • Drug for treating tumor
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Examples

Experimental program
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Effect test

Embodiment 1

[0059] [Example 1]: Observation of morphology and particle size determination of cell vesicles coated with chemotherapeutic drugs obtained after UV-induced apoptosis of mouse liver cancer cells and mouse erythrocytes to produce cell vesicles and chemotherapeutic drugs

[0060] 1. Experimental materials and reagents

[0061] H22 mouse liver cancer cells, normal mouse erythrocytes, methotrexate.

[0062] 2. Experimental steps

[0063] 1) Cultivate H22 mouse liver cancer cells with 1640 cell culture medium to make the total number of cells reach 3×10 8;Collect a certain amount of normal mouse blood to the anticoagulant blood vessel, add the same amount of PBS, and mix well. Centrifuge at 2000rpm for 5 minutes, and remove the supernatant. with no Ca 2+ , Mg 2+ Wash the red blood cells with Hank's solution for 3 times, centrifuge at 2000rpm for 5 minutes for the first 2 times, and centrifuge for 10 minutes at 2000rpm for the last time, and remove the supernatant. Select a hem...

Embodiment 2

[0078] [Example 2]: Detection of H22 cell-derived vesicles and mouse erythrocyte-derived vesicle-encapsulated chemotherapeutic drugs (methotrexate)

[0079] 1. Experimental materials and reagents

[0080] H22 mouse liver cancer cells, mouse red blood cells, methotrexate, high performance liquid chromatography.

[0081] 2. Experimental steps

[0082] 1) Cultivate H22 mouse liver cancer cells with 1640 cell culture medium, so that the total number of cells reaches 1×10 8 According to the method of Example 1, mouse blood was collected, red blood cells were separated, and the concentration of red blood cells was calculated. Take a total of 1×10 from it 8 mouse red blood cells.

[0083] 2) According to the method of Example 1, H22-MPs (MTX) and Ery-MPs (MTX) were prepared, separated and extracted respectively.

[0084] 3) Count H22-MPs (MTX) and Ery-MPs (MTX) according to the method in Example 1, and then calculate the concentrations of H22-MPs (MTX) solution and Ery-MPs (MTX)...

Embodiment 3

[0091] [Example 3]: The killing effect of methotrexate encapsulated in vesicles produced by apoptosis of human erythrocytes on various tumor cells

[0092] 1. Experimental materials and reagents

[0093] Different human tumor cell lines include: A2780 human ovarian cancer cell line, human breast cancer cell line MCF-7, human lung cancer cell line A549, human gastric cancer cell line MGC-803, human colorectal cancer T84, human liver cancer cell line HepG2, human ovarian cancer cell line Cancer cell line Ho-8910, human cervical cancer cell line Hela, human prostate cell line PC-3, human esophageal cancer cell line EC109, human nasopharyngeal cancer cell line CNE, human kidney cancer cell line A498, human skin squamous cell line A-431, human lymphoblastoid NCI-BL2009, human fibrosarcoma carcinoma cell HT-1080, human bladder cancer cell line T24, human leukemia cell line K562, human promyelocytic leukemia cell HL60, human melanoma cell A875, human acute Lymphocytic leukemia cell ...

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Abstract

The invention provides an erythrocyte vesicle-derived drug for treating tumor, comprising erythrocyte vesicles and a therapeutic drug encapsulated in the erythrocyte vesicles. The erythrocyte vesiclesare vesicles released by apoptotic erythrocytes, and the therapeutic drug is a tumor therapeutic drug as an effective component for treating tumors. As the targeting drug, the drug can better targeton a tumor part, increases the pharmaceutical effect for chemotherapeutic drugs, and greatly mitigates the toxic and side effect of drug delivery and administration using an exogenous carrier on a body.

Description

technical field [0001] The invention relates to a drug for treating tumors, in particular to a tumor chemotherapy drug derived from erythrocyte vesicles. Background technique [0002] As the ecological environment in which humans live is deteriorating, the incidence of human tumors is high, and tumors have become a major disease that seriously threatens human health. How to effectively treat them has always been a subject of scientific research. Among them, chemotherapy is the most conventional cancer treatment method. While destroying tumor cells, it also kills normal cells, which not only brings serious toxic and side effects to cancer patients, but often even leads to the failure of chemotherapy for cancer patients. [0003] The essence of the toxic and side effects of chemotherapy drugs on the body is the killing of normal tissue cells by chemotherapy drugs. In order to effectively avoid the non-specific killing of normal tissue cells by chemotherapeutic drugs, people u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/18A61K45/06A61P35/00A61K31/519
CPCA61K31/519A61K35/18A61K45/06A61K2300/00A61K9/10A61K47/46A61P35/00A61P35/02A61K9/1664A61K9/1694
Inventor 黄波张一唐科
Owner HUBEI SOUNDNY BIOLOGICAL TECH
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