A carbon nanomaterial for rapid detection of residual chlorine in water, its supporting device and its use method
A carbon nanomaterial and water detection technology, applied in analytical chemistry and nanometer fields, can solve the problems of high price, inability to online monitoring, fluorescence quenching, etc., and achieve the effects of simple operation, wide application range and low cost
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Embodiment 1
[0024] Example 1: carbon dots m -Synthesis of CDs
[0025] Firstly, 0.6 g of m-phenylenediamine was dissolved in 60 mL of absolute ethanol, and then the solution was transferred to a 100 mL polytetrafluoroethylene high-temperature reactor, and the reaction was heated at 180 °C for 12 h, and the reactor was cooled to room temperature. Finally, the resulting product is separated and purified by column chromatography (eluent is dichloromethane:methanol=15:1, v / v), and the obtained solution is spin-dried with a rotary evaporator and then vacuum-dried, that is get carbon dots m -Solid crystals of CDs ( m -Relevant characterization of CDs such as figure 1 , where A is m - Transmission electron microscope image of CDs, showing that the particle size distribution is 4-6 nm; B is m -Atomic force microscope image of CDs, characterizing its height; C is corresponding to B m -The height of CDs is displayed as about 1.5 nm; D is the ultraviolet absorption diagram, fluorescence excita...
Embodiment 2
[0026] Example 2: ClO - Drawing of standard curve
[0027] Use PBS (pH=7.0) buffer to prepare different concentrations of ClO - Solution (ClO - The concentration is 0, 0.05, 0.1, 0.25, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15 μM), and then at room temperature, to 20 μg / mL m -Add different concentrations of ClO to the CDs solution sequentially - Solution, after mixing and reacting for 5 min m -CDs fluorescence intensity changes and plotted with respect to ClO - Standard curve of response (eg figure 2 , where A is different concentrations of ClO - and m -The change graph of fluorescence intensity after CDs reaction; B is m -CDs respond to different concentrations of ClO - A standard curve with a response range of 0.05-7 μM and a limit of detection (LOD) of 0.012 μM).
Embodiment 3
[0028] Example 3: m - Preparation of CDs agarose hydrogel
[0029] Take a certain volume of 20 μg / mL m- CDs solution, add agarose powder to it, and make the agarose evenly distributed in the solution by magnetic stirring, then heat in a microwave oven until the agarose is completely dissolved and forms a viscous colloid, take 300 μL of the sol and drop it evenly on the centrifuge tube cover In the groove, after it cools to room temperature, it forms a round cake m - CDs agarose hydrogel. Take it out and put it in a centrifuge tube filled with distilled water and store it in the refrigerator at 4°C for later use.
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