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Method for easily, conveniently and quickly measuring non-sugar solid matters in yellow rice wine with alcohol content ranging from 18 degrees to 20 degrees

A non-sugar solid, rapid determination technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of high detection cost, time-consuming, unfavorable rapid determination, accurate measurement and batch detection, etc., to achieve good accuracy and reliability , easy and quick operation, low cost detection effect

Inactive Publication Date: 2018-03-16
洪佳欣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current national standard of rice wine uses the 4-hour drying method to measure the non-sugar solid content in the wine sample. This method takes a long time, and there are cases where the quality of the wine sample does not reach a constant weight after drying for 4 hours, which is not conducive to rapid determination. , precise measurement and batch testing
In addition, there is a rapid determination method for non-sugar solids in wine samples based on near-infrared spectroscopy, but this method requires expensive equipment and high detection costs

Method used

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  • Method for easily, conveniently and quickly measuring non-sugar solid matters in yellow rice wine with alcohol content ranging from 18 degrees to 20 degrees

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 is the preparation of a standard curve for the determination of the mass-to-volume ratio of water and ethanol in the simple and rapid determination method for the non-sugar solids of rice wine with 18 to 20 degrees of the present invention.

[0019] (1) Preparation of artificial ethanol water system

[0020] Use a pipette to measure 18.00ml, 18.40ml, 18.80ml, 19.20ml, 19.60ml and 20.00ml of absolute ethanol into six 100ml volumetric flasks, and place the volumetric flasks on the analytical balance to add 82.00g and 81.60g respectively , 81.20g, 80.80g, 80.40g, and 80.00g of distilled water, tightly capped the volumetric bottle cap, repeatedly inverted and fully mixed, left at room temperature for 10 minutes, and finally obtained different mass-volume ratios of water and ethanol, that is, the mass of water / volume of ethanol (corresponding to 4.556, 4.435, 4.319, 4.208, 4.102 and 4.000) artificial ethanol water system.

[0021] (2) Pretreatment of artificial et...

Embodiment 2

[0030] Example 2 is the actual determination of the non-sugar solids of rice wine samples marked with an alcohol content of 18 by using the new method of the present invention.

[0031] (1) Choose commercially available Lanling Fine Wine (produced by Shandong Lanling Fine Wine Co., Ltd.), the alcohol content of which is marked on the bottle is 18%. For the sake of accuracy, the alcohol content needs to be measured again, so the alcohol content of this yellow rice wine is determined to be 18.1 based on the current national standard of rice wine with an alcohol meter. After weighing and conversion, the mass of 1L of rice wine is 1081.538g. The temperature of the wine sample is 30°C, and the density of ethanol at this temperature is 0.781g / ml.

[0032] (2) Pretreatment of rice wine samples to be tested

[0033] Use a pipette gun to pipette 320ul of the rice wine sample to be tested into an empty common microplate, first add 40ul of a mixed aqueous solution of 15% by mass volume...

Embodiment 3

[0041] Example 3 is the actual determination of the non-sugar solids of rice wine samples marked with an alcohol content of 20 by using the new method of the present invention.

[0042] Choose commercially available Longyan Shengang Liquor (manufactured by Longyan Shengang Liquor Industry Co., Ltd.), and the alcohol content marked on the bottle is 20 degrees. For the sake of accuracy, the alcohol content needs to be measured again, so based on the current national standard of rice wine, the alcohol content of this rice wine is determined to be 19.9 by the alcohol meter method. After weighing and conversion, the mass of 1L of rice wine is 1084.837g. The temperature of the wine sample is 30°C, and the density of ethanol at this temperature is 0.781g / ml.

[0043] Measured with reference to Example 2, the actual absorbance x=0.238±0.003, the relevant values ​​are shown in Table 3. If the total solids content measured by the national standard method is used as a benchmark, the va...

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Abstract

The invention discloses a novel method for measuring non-sugar solid matters in yellow rice wine with the alcohol content ranging from 18 degrees to 20 degrees. The novel method is established by theaid of difference in absorbance at the wave lengths of 260 nm caused by the difference of denaturation degrees of specific double-stranded DNA (deoxyribonucleic acid) fragments in ethyl alcohol watersystems with different water to ethyl alcohol mass and volume ratios. The specific double-stranded DNA fragments are shown as SEQ ID NO:1, and a relational expression of the absorbance x of the double-stranded DNA fragments and the mass and volume ratios of water and ethyl alcohol in the ethyl alcohol water systems is y=-4.7652*x+5.152. The novel method for measuring the non-sugar solid matters has the advantages that the novel method is applicable to measuring the yellow rice wine with the alcohol content of 18-20 degrees, is excellent in accuracy and reliability and low in detection cost, issafe and can be easily, conveniently and quickly implemented, long-time drying can be omitted, and only low wine sample volumes are required.

Description

technical field [0001] The invention relates to a method for detecting non-sugar solids, in particular to a simple and rapid method for determining the non-sugar solids in 18-20 degree yellow rice wine. Background technique [0002] Yellow rice wine is a special wine in my country. It has bright color, rich aroma, sweet taste, mellow flavor, and is rich in amino acids, small molecular sugars, organic acids, vitamins and trace elements. Rice wine is deeply loved by the masses of the people because of its rich nutrition, low alcohol content and unique flavor, mouthfeel and efficacy. Non-sugar solids are a general term for non-volatile components other than sugar in wine samples, and their proportion in rice wine is very small, usually less than 3%. Although the content is low, the non-sugar solids have a great effect. It determines the color, taste, nutrition and efficacy of rice wine to a large extent, and is an important index for the quality evaluation of rice wine. Accor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 洪佳欣陈如凯
Owner 洪佳欣
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