Factory breeding method for virus-free seedlings of salviae miltiorrhizae

A technology of virus-free seedlings and salvia miltiorrhiza, applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve problems such as unstable production of salvia miltiorrhiza and degradation of seedling vitality, and achieve prevention and control of salvia miltiorrhiza virus and well-developed root system , the effect of high detoxification rate

Pending Publication Date: 2018-03-20
许文胜
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, the salvia miltiorrhiza supplied in the market is mainly cultivated species, but in the main production areas of salvia mil...

Method used

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  • Factory breeding method for virus-free seedlings of salviae miltiorrhizae
  • Factory breeding method for virus-free seedlings of salviae miltiorrhizae
  • Factory breeding method for virus-free seedlings of salviae miltiorrhizae

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Materials

[0055] 1. Salvia miltiorrhiza: Triploid salvia miltiorrhiza (cultivated by the chromosome laboratory of Nankai University, Research on the cultivation and sustainable utilization of triploid salvia miltiorrhiza, Chinese Herbal Medicine, February 2012, Volume 43, No. 2, 375-379)

[0056] 2. Mercury: purchased from Shanghai Biological Engineering Co., Ltd.

[0057] 3. Initial proliferation medium: 1 / 2MS, 30g / L sugar, 6g / L agar, 0.5mg / L 6-BA, 0.2mg / L NAA, pH 5.8. Prepared by weight / volume ratio, the solvent is water. Use after routine autoclaving.

[0058] 4. Dedifferentiation proliferation medium: 1 / 2MS, 30g / L white sugar, 6g / L agar, 1mg / L 6-BA, pH 5.8. Prepared by weight / volume ratio, the solvent is water. Use after routine autoclaving.

[0059] 5. Induction medium: 1 / 2MS, 30g / L sugar, 6g / L agar, 0.2mg / L NaA, pH 5.8. The solvent is water.

[0060] 6. Root induction differentiation medium: 1 / 2MS, 30g / L white sugar, 6g / L agar, 0.5mg / L IBA, pH 5.8. Pr...

Embodiment 2

[0071] 1. Materials

[0072] 1. Salvia miltiorrhiza: triploid salvia miltiorrhiza (cultivated by the chromosome laboratory of Nankai University, (cultivated by the chromosome laboratory of Nankai University, research on the cultivation and sustainable utilization of triploid salvia miltiorrhiza), Chinese herbal medicine, February 2012, volume 43 Issue 2, 375-379))

[0073] 2. Mercury: purchased from Shanghai Biological Engineering Co., Ltd.

[0074] 3. Initial proliferation medium: 1 / 2MS, 25g / L sugar, 6g / L agar powder, 0.5mg / L 6-BA, 0.2mg / L IBA, pH 5.8. Prepared by weight / volume ratio, the solvent is water. It was filtered with a 22 μm microporous membrane and used after conventional autoclaving.

[0075] 4. Dedifferentiation proliferation medium: 1 / 2MS, 25 / L sugar, 6.5g / L agar powder, 1mg / L 6-BA, pH 5.8. Prepared by weight / volume ratio, the solvent is water. Use after routine autoclaving.

[0076] 5. Induction medium: 1 / 2MS, 25g / L sugar, 6.5g / L agar powder, 0.2mg / L IBA,...

Embodiment 3

[0087] Embodiment 3 Salvia miltiorrhiza root virus-free seedling industrialized breeding culture medium

[0088] Consists of one or more of the following media:

[0089] 1. Initial proliferation medium: 1 / 2MS, 30g / L sugar, 6.2g / L agar powder, 0.5mg / L 6-BA, 0.2mg / LNAA, pH 5.8. Prepared by weight / volume ratio, the solvent is water. Use after routine autoclaving.

[0090] 2. Dedifferentiation proliferation medium: 1 / 2MS, 30g / L sugar, 6.2g / L agar, 1mg / L 6-BA, pH 5.8. Prepared by weight / volume ratio, the solvent is water. Use after routine autoclaving.

[0091] 3. Induction medium: 1 / 2MS, 30g / L sugar, 6.2g / L agar powder, 0.2mg / L NAA, pH 5.8. The solvent is water.

[0092] 4. Root induction differentiation medium: 1 / 2MS, 30g / L white sugar, 6.2g / L agar, 0.2mg / L IBA, pH 5.8. Prepared according to weight / volume ratio, and used after filtration with 22μm microporous membrane and conventional autoclaving. The solvent is water.

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Abstract

The invention relates to a factory breeding method for virus-free seedlings of salviae miltiorrhizae, in particular to a factory breeding method for wild species, cultivated species or varied speciesof salviae miltiorrhizae which is severe in quality degradation, lower in yield and short in resources, and belongs to the technical field of plant varieties breeding. The factory breeding method forvirus-free seedlings of salviae miltiorrhizae comprises the following steps: (1) selecting tender leaves or stem tips of the salviae miltiorrhizae to perform virus-free treatment; (2) culturing the tender leaves or stem tips of the salviae miltiorrhizae in an initial inducible-proliferation culture medium; (3) performing differentiation culture on salviae miltiorrhizae callus in a differentiationculture medium; (4) performing root induced culture on the seedlings of salviae miltiorrhizae in a root inducing culture medium; (5) exercising the seedlings of salviae miltiorrhizae; and (6) performing field transplanting for seedlings of salviae miltiorrhizae. The factory breeding method can realize quick and factory breeding for excellent salviae miltiorrhizae variety (system) or strain systemand cherish endangered salviae miltiorrhizae resources.

Description

technical field [0001] The invention relates to an industrial breeding method for virus-free seedlings of Salvia miltiorrhiza, in particular to an industrial breeding method for wild species, cultivated species or mutant species of Salvia miltiorrhiza with severe quality degradation, reduced yield and scarce resources, and belongs to the technical field of plant variety breeding. Background technique [0002] Danshen (Salvia miltiorrhza Bunge) is one of the bulk of traditional Chinese medicinal materials, which has a significant effect of promoting blood circulation and removing blood stasis, and is widely used in the treatment of coronary heart disease, angina pectoris, ischemic stroke and other diseases. At present, there are more than 100 compound Chinese medicines produced from Danshen as raw materials in my country, involving more than 100 enterprises, and there is a huge demand for high-quality Danshen medicinal materials. However, due to perennial excavation, the wild...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 许文胜
Owner 许文胜
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