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Application of a series of compounds with tannic acid as representation in resisting infection of chikungunya and other alphaviruses

A technology of chikungunya virus, tannic acid, applied in the field of pharmacy

Inactive Publication Date: 2018-03-23
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] But so far, the application of tannic acid, oxytetracycline sulfosalicylic acid, 2,3-dimercaptosuccinic acid and oritavancin diphosphate in anti-CHIKV and other alphavirus infections has not been reported.

Method used

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  • Application of a series of compounds with tannic acid as representation in resisting infection of chikungunya and other alphaviruses
  • Application of a series of compounds with tannic acid as representation in resisting infection of chikungunya and other alphaviruses
  • Application of a series of compounds with tannic acid as representation in resisting infection of chikungunya and other alphaviruses

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Embodiment Construction

[0027] In order to better illustrate the present invention, specific embodiments of the present invention will be described in detail below.

[0028] 1. Expression and purification of CHIKV nsP2 protease

[0029] According to literature (Das, P.K., Merits, A. & Lulla, A. Functional Cross-talk between Distant Domains of Chikungunya Virus Non-structural Protein 2 Is Decisive for Its RNA-modulating Activity. J. Biol. Chem. 289, 5635–5653 (2014 )),(RAUSALUK,UTTA,QUIRIN T et al..Chikungunya virus infectivity,RNA replication and non-structural polyprotein processing depend on the nsP2 protease's active sitecysteine ​​residue[J].Scientific Reports,2016,6(October):37124. ) method to express and purify CHIKV nsP2 protease.

[0030] Specific steps are as follows:

[0031] (1) Transform the pET28a vector containing the gene encoding CHIKV nsP2 protease into the strain of Escherichia coliBL21 (DE3), and use LB plate medium (containing 50 mg / L kanamycin) to screen positive clones.

[00...

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Abstract

The invention provides an application of a series of compounds with tannic acid as representation in resisting infection of chikungunya and other alphaviruses, and specifically relates to the application of tannic acid, meclocycline sulfosalicylate, 2,3-dimercaptosuccinic acid and oritavancin diphosphate in resisting infection of chikungunya and other alphaviruses; the compounds can be used for inhibition of the activity of chikungunya virus nsP2 protease (protease of a nonstructural protein 2), and are expected to become potential drugs for resisting infection of CHIKV and other alphaviruses.

Description

technical field [0001] The present invention relates to the technical field of pharmacy, in particular, relates to tannic acid, oxytetracycline sulfosalicylic acid, 2,3-dimercaptosuccinic acid and oritavancin diphosphate in the anti-gekungunya Applications in alphavirus infection. Background technique [0002] Chikungunya fever is an infectious disease caused by Chikungunya virus infection. Chikungunya virus (chikungunya virus), referred to as CHIKV, is mainly transmitted through the bite of Aedes mosquitoes and can cause fever, joint pain, headache, rash, and muscle pain. Among them, joint pain and fever are the most common symptoms of CHIKV infection, and joint pain can last for several months Even longer time brings serious health threat to patient. [0003] Chikungunya first broke out in Tanzania in 1952 and the virus was isolated in 1953. The name "chikungunya" is derived from the local dialect, which means "flexion pain" and is used to describe the joint pain caused...

Claims

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Application Information

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IPC IPC(8): A61K38/14A61K31/7024A61K31/65A61K31/194A61P31/14
CPCA61K31/194A61K31/65A61K31/7024A61K38/14Y02A50/30
Inventor 杨海涛王赛男刘祥王泽方陈成蔡岩郑金姣
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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