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Improved lactobacillus growth and metabolism culture medium and using method thereof

A culture medium, lactic acid bacteria technology, applied in bacteria, fermentation and other directions, can solve problems such as failure to meet activation requirements, insufficient growth density, high lactic acid production, etc., achieve effective and reasonable formula design, promote the growth of lactic acid bacteria, and improve the effect of lactic acid production.

Inactive Publication Date: 2018-03-23
JIANGSU HENGSHUN VINEGAR IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the nutrients are not enough, the growth of the bacteria will be slow or stagnant after inoculation, and the activation requirements will not be met or the growth density will not be enough to affect the fermentation production.
The traditional lactic acid bacteria medium lacks some trace elements, so that lactic acid bacteria cannot grow rapidly. First, a process of feeding lactic acid bacteria during the growth process is invented, so as to promote the growth and metabolism of lactic acid bacteria and increase the production of lactic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] This embodiment proposes a kind of improved culture medium formula of lactic acid bacteria, and its component ratio by weight is:

[0017] 8 parts of peptone, 13 parts of beef extract, 70 parts of glucose, 3.5 parts of dipotassium hydrogen phosphate, 0.6 parts of magnesium sulfate, 4 parts of sodium acetate, 3 parts of ammonium citrate, 0.05 parts of manganese sulfate monohydrate, 1 part of Tween-80, 900-1000 parts of distilled water, pH6.0.

[0018] After being prepared according to the above formula, the medium was autoclaved at 121.3° C. for 20 minutes, and after the sterilization was completed, it was dried for later use to obtain an improved medium.

[0019] Wash the potatoes, dry them and cut them into pieces, then use a hand-cranked meat grinder to make mashed potatoes, filter all the substances with 100-mesh gauze, collect the filtered slurry in a large bottle, add water, and the mass ratio of water is 1:10-1:20, after stirring, sieve with a fine sieve of 2mm a...

Embodiment 2

[0027] This embodiment proposes a kind of improved culture medium formula of lactic acid bacteria, and its component ratio by weight is:

[0028] 9 parts of peptone, 12 parts of beef extract, 75 parts of glucose, 4 parts of dipotassium hydrogen phosphate, 0.5 parts of magnesium sulfate, 3.5 parts of sodium acetate, 4 parts of ammonium citrate, 0.06 parts of manganese sulfate monohydrate, 2 parts of Tween-80, 900-1000 parts of distilled water, pH6.2.

[0029] After being prepared according to the above formula, the medium was autoclaved at 121.3° C. for 20 minutes, and after the sterilization was completed, it was dried for later use to obtain an improved medium.

[0030] Lactic acid bacteria culture steps are:

[0031] (1) prepare the culture medium of the improved type that Lactobacillus helveticus is adapted to grow;

[0032] (2) Return the strains from the -80°C refrigerated state to room temperature, and thaw the strains for subsequent activation of the strains;

[0033...

Embodiment 3

[0037] This embodiment proposes a kind of improved culture medium formula of lactic acid bacteria, and its component ratio by weight is:

[0038] 10 parts of peptone, 15 parts of beef extract, 68 parts of glucose, 3.8 parts of dipotassium hydrogen phosphate, 0.55 parts of magnesium sulfate, 3 parts of sodium acetate, 3.5 parts of ammonium citrate, 0.56 parts of manganese sulfate monohydrate, 1.2 parts of Tween-80, 900-1000 parts of distilled water, pH6.5.

[0039] After being prepared according to the above formula, the medium was autoclaved at 121.3° C. for 20 minutes, and after the sterilization was completed, it was dried for later use to obtain an improved medium.

[0040] Lactic acid bacteria culture steps are:

[0041] (1) prepare the culture medium of the improved type that Lactobacillus helveticus is adapted to grow;

[0042] (2) Return the strains from the -80°C refrigerated state to room temperature, and thaw the strains for subsequent activation of the strains;

...

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PUM

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Abstract

The invention discloses an improved lactobacillus growth and metabolism culture medium. The improved lactobacillus growth and metabolism culture medium comprises the following components in parts by weight: 5-12 parts of peptone, 10-15 parts of beef extract, 60-80 parts of glucose, 2-4 parts of dipotassium phosphate, 0.5-0.8 part of magnesium sulfate, 2-5 parts of sodium acetate, 2-4 parts of ammonium citrate, 0.03-0.06 part of manganese sulfate monohydrate, 0.6-2 parts of tween-80, and 900-1,000 parts of distilled water. PH value is 5.6-6.5, and 1-3 parts by weight of a potato extracting solution is supplemented in the components in a fermenting process. The formula is effective and reasonable in design, growth of lactobacillus can be promoted effectively, metabolites are secreted efficiently, fermentation and production are promoted, and the yield of lactic acid is increased.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to an improved medium for the growth and metabolism of lactic acid bacteria. Background technique [0002] The recovery and activation of lactic acid bacteria and fermentation culture require a suitable medium, which must contain appropriate nitrogen sources, carbon sources, trace elements, inorganic salts, vitamins and other nutrients, so that lactic acid bacteria can be rapidly activated and rapidly grown and fermented. If the nutrients are not enough, the growth of the bacteria will be slow or stagnant after inoculation, and the activation requirements will not be met or the growth density will not be enough to affect the fermentation production. The traditional lactic acid bacteria medium lacks some trace elements, so that lactic acid bacteria cannot grow rapidly. First, a process of feeding lactic acid bacteria during the growth process is invented, so as to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56
CPCC12N1/20C12P7/56
Inventor 姚清余永建李信朱胜虎奚宽鹏李童
Owner JIANGSU HENGSHUN VINEGAR IND
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