Split bioreactor

Abstract

The invention relates to a split bioreactor, which is composed of a pressurized cabin, a culture cabin, and a pressurized sealing device. Several culture cabins with independent sealing structures can be placed in the pressurization cabin. By replacing the culture cabin, the The use efficiency of the pressurized cabin; the pressurized cabin box is equipped with pressurized gas inlet and outlet pipes, and the contact between the pressurized cabin cover and the pressurized cabin box is sealed and connected by a gasket, and the pressurized cabin cover is fixed with a quick connector On the box body of the pressurized cabin; the pressurized sealing devices corresponding to the training cabin are respectively installed on the pressurized hatch cover, and the mechanical ring gripper is connected with the guide hole in the pressurized hatch cover through the transmission rod on it, and the transmission rod is connected with the pressurized hatch cover. The guide holes in the ballast cover are sealed and connected by seals. When the pressurization chamber is pressurized, the mechanical ring gripper is driven by the pressurization sealing device to press tightly on the silica gel diaphragm of the cultivation chamber, so that the silica gel diaphragm It forms a seal with the upper end surface of the culture tank, so that the liquid will not overflow when the pressurized chamber is pressurized, and safe aseptic operation can be achieved.

Description

technical field [0001] The invention relates to a bioreactor, in particular to a split type bioreactor, and belongs to the technical field of bioreactors. Background technique [0002] A bioreactor is an experimental tool used to cultivate biological cells and tissues. It provides cells and tissues with a high-pressure culture environment for 1 hour a day. One blow, aseptic (this is the basis of cell culture), sufficient nutritional environment (including the supply of nutrients (the degradation rate of various cells must be fast) and the removal of waste), to meet the needs of biological tissues every other day liquid. [0003] Integrated bioreactor with existing circulation system and pressurization system. Such as patent publication number CN103589638B, a pneumatic self-circulation animal cell culture bioreactor and its use method, the magnetic stirring system, heating system and control system located at the bottom of the large tank, the upper end of the large tank is ...

AI Technical Summary

Problems solved by technology

The operation is complex, prone to human error, resulting in liquid leakage, once the liquid leaks, it will cause pollution

The integrated design does not allow routine liquid changes every other day, and cannot meet the nutritional conditions of the cell and tissue culture environment

The integrated design results in a bulky pressurized chamber that carries cultured tissues in the incubator for long-term cultivation. The humid and hot environment in the incubator causes rapid aging of components, reduces the stability of the equipment, and increases the risk of contamination

The integrated design makes the operation of putting cells and tissues into the reactor and taking them out of the reactor complicated, and it is an operation with a high risk of contamination

Integrated design, in order to achieve the safety conditions of the internal pressurized chamber in a high-pressure environment, the reactor is designed to be heavy, and it is difficult to carry and operate for practical operation

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