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Field identification method for evaluating aspergillus flavus infection resistance of corn

An identification method and technology of Aspergillus flavus, applied in the agricultural field, can solve the problems of inability to directly act on grains, difficult to cause pathogenic relationships, etc., and achieve the effects of ensuring test reproducibility, avoiding area parameter errors, and reducing systematic errors.

Inactive Publication Date: 2018-04-03
INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And methods such as filament spraying cannot directly act on the seeds, and it is difficult to have an obvious direct pathogenic relationship
There are many domestic studies on the pathogenic mechanism and resistance mechanism of corn grain in the laboratory. The field research method mainly refers to the inoculation method of peanuts. There is no complete and feasible research method for the resistance of Aspergillus flavus in corn.

Method used

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  • Field identification method for evaluating aspergillus flavus infection resistance of corn
  • Field identification method for evaluating aspergillus flavus infection resistance of corn
  • Field identification method for evaluating aspergillus flavus infection resistance of corn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Spore suspension concentration parameter optimization in the Aspergillus flavus inoculation method of embodiment 1

[0022] This example aims to investigate the optimum spore suspension concentration for inoculating corn ears with Aspergillus flavus spores to cause disease. The test process is as follows: 1) Streak inoculation of Aspergillus flavus (3.4410) on the Cha's solid medium; 2) After culturing at 28°C for 7 days, the spores of Aspergillus flavus on the surface of the medium were eluted with distilled water, and filtered to prepare Aspergillus flavus spores suspension. Set five spore concentration gradients, the concentration is 1.0×10 4 pcs / ml, 1.0×10 5 pcs / ml, 1.0×10 6 pcs / ml, 1.0×10 7 pcs / ml, 1.0×10 8 pieces / ml. 3) Within 15 to 20 days after corn ear pollination, use a syringe to inoculate the spores of Aspergillus flavus, inoculate 0.5ml of spore suspension of different concentrations in the upper, middle and lower three parts of the corn ear with a th...

Embodiment 2

[0027] Example 2 Identification of Resistance Ability of Different Maize Inbred Lines to Aspergillus Aflatoxin Infection

[0028] The purpose of this example is to investigate the identification of the resistance of different maize inbred lines to Aspergillus flavus infection in field experiments. The test process is as follows:

[0029] 1) Streak inoculation of Aspergillus flavus (3.4410) on the Cha's solid medium;

[0030] 2) After culturing at 28°C for 7 days, the Aspergillus flavus spores were eluted with distilled water and filtered to prepare a suspension of Aspergillus flavus spores at a concentration of 1.0×10 6 a / ml;

[0031] 3) Within 15 to 20 days after corn ear pollination, use a syringe to inoculate the spores of Aspergillus flavus, inoculate 0.5ml of the spore suspension in the upper, middle, and lower three parts of the corn ear, and insert the needle into the bract leaf of the corn ear obliquely at 45 degrees. Pierce a single kernel.

[0032] 4) 12 corn inb...

Embodiment 3

[0041] Example 3 Comparison of implementation differences between top injection inoculation method, row needle inoculation method and three-point spiral inoculation method

[0042] This example aims to compare the infection effects of three Aspergillus flavus inoculation methods on corn inbred lines, and optimize and obtain the best inoculation method.

[0043] Method 1, the top injection method is a non-injurious inoculation method. In this embodiment, in order to ensure that the inoculation amounts of the three methods are the same, on the 15th day after the ear pollination, 1.5ml of Aspergillus flavus spores with a concentration of 1.0×106 / ml The suspension is injected into the bracts from the middle of the top filaments to avoid damage to the top seeds.

[0044] Method 2, the row needle inoculation method is to use the needle to dip the suspension of Aspergillus flavus spores with a concentration of 1.0×106 / ml on the 15th day after the pollination of the ear, and insert th...

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Abstract

The invention provides a field identification method for evaluating aspergillus flavus infection resistance of corn. The method comprises steps as follows: 1), a czapek's solid medium is inoculated with aspergillus flavus; 2), aspergillus flavus spores on the surface of the medium are scraped off, and aspergillus flavus spore suspension with concentration being 1.0*10<6> / ml is prepared from distilled water; 3), the aspergillus flavus spores are inoculated by a scale syringe in 15-20 days after pollination of corn ears, the upper part, the middle part and the lower part of each corn ear are inoculated with 0.5 ml of spore suspension respectively, and a syringe needle is obliquely pierced into a bracteal leaf of the corn ear with 45 degrees; 4), resistance of a corn selfing line to aspergillus flavus infection is determined according to ear incidence area and aflatoxin B1 content. Compared with the prior art, the method is based on the natural environment of the corn growing field and meets the agricultural production rule, an identification result is accurate, the stability is high, and the method has no adverse effect on body health.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method for field inoculation of Aspergillus flavus in corn and an identification method for evaluating the ability to resist infection by Aspergillus flavus. Background technique [0002] my country is a large corn-growing country, and the maize growth process and climate resources in different ecological zones are quite different. During the ripening period of corn in Huanghuaihai, the rain and heat are synchronized, which is characterized by high temperature and high humidity. The ears are easily contaminated by Aspergillus flavus during the filling and dehydration process, and a large amount of aflatoxin is easy to accumulate in the field, which affects the quality of corn and endangers the health of humans and animals. Aflatoxin (AF) is a general term for a class of compounds with similar structures. It is a biologically active substance produced by Aspergillus flavus...

Claims

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Application Information

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IPC IPC(8): G01N33/00G01N33/569G01N33/535G01N33/543
Inventor 齐耀程丁舰舟阮龙左晓龙钱益亮任四海王俊张玮
Owner INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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