Application of fermented compound of Aspergillus terreus h768 in the preparation of antiallergic drugs

A technology of H768, compound, applied in the application field of extracting compounds from marine fungi, can solve the problem of not finding an active drug that inhibits allergenic food antigens, etc., and achieves good application prospects, strong anti-allergic activity, and the effect of promoting effective application.

Active Publication Date: 2020-05-15
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current treatment for food allergy is to avoid exposure to allergens or perform desensitization therapy, and no active drug that inhibits allergenic food antigens has been found
Dietary intervention is an important method for the management of food allergy, but it will have a negative impact on the nutritional intake and quality of life of the human body; and the use of antihistamines or hormonal drugs is prone to side effects such as drug resistance, so looking for anti-food allergy activity Compounds are especially important

Method used

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  • Application of fermented compound of Aspergillus terreus h768 in the preparation of antiallergic drugs
  • Application of fermented compound of Aspergillus terreus h768 in the preparation of antiallergic drugs
  • Application of fermented compound of Aspergillus terreus h768 in the preparation of antiallergic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In this embodiment, Aspergillus terreus H768 (Aspergillus terreus H768) in the deep-sea fungus is used as a raw material to prepare the aspergillus fermentation compound shown in formula (I), and the specific steps are as follows:

[0020] (1) Take Aspergillus terreus H768, add it to the culture medium, let it stand for 35 days, add an organic solvent to extract three times, combine to obtain the extract, concentrate the extract under low pressure, and obtain 28.6g of Aspergillus fermented crude extract;

[0021] (2) performing ODS chromatographic column adsorption on the Aspergillus fermented crude extract in step (1), and carrying out gradient elution with aqueous methanol;

[0022] (3) Adsorb the active site obtained in step (2) with Sephadex LH-20, and use 1:1 chloroform-methanol solution to elute;

[0023] (4) The active site obtained in step (3) is subjected to 100-200 mesh silica gel column chromatography adsorption, and 10:0 to 1:1 chloroform-methanol solution i...

Embodiment 2

[0028] Example 2 In vitro anti-food allergy activity screening of the compound prepared in Example 1

[0029] In this example, the IgE-mediated RBL-2H3 cell model was selected, the degranulation efficiency of the cells after sensitization was detected, and the inhibition rate of the compound sample on the degranulation efficiency of the cells was calculated, so as to detect the anti-food allergy activity of Compound 1 in Example 1.

[0030] The present embodiment is divided into following 4 groups:

[0031] (1) Inactive group;

[0032] (2) DNP-BSA activation group;

[0033] (3) Positive control group, loratadine;

[0034] (4) Aspergillus fermentation compound experimental group: Aspergillus fermentation compound obtained in step (5) in Example 1;

[0035] This embodiment adopts the following operations:

[0036] (1) RBL-2H3 cells were recovered by trypsinization, 6×10 5 cells / mL, add 100 μL to each well (ie 6×10 4 cells / well) into a 96-well plate, while adding anti-DNP-I...

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Abstract

The invention discloses the application of the fermented compound of Aspergillus terreus H768 in the preparation of antiallergic medicine. Aspergillus terreus fermentation compound of the present invention is obtained by fermentation and extraction of Aspergillus terreus H768, and its structural formula is as shown in formula (I): Aspergillus terreus H768 (Aspergillus terreus H768), preserved in China Type Culture Collection Center, preservation number It is CCTCC NO: M 2017121, and the deposit address is China. Wuhan. Wuhan University, and the deposit date is March 15, 2017; the invention finds that the Aspergillus fermented compound has strong anti-allergic activity and can be used to prepare anti-allergic drugs. The invention provides a new source of compounds for the research and development of anti-allergic drugs, which is of great significance to the development of marine drugs in my country, and can also promote the effective application of deep-sea biological resources, and has a good application prospect.

Description

technical field [0001] The invention belongs to the application field of compounds extracted from marine fungi, more specifically, the invention relates to the application of Aspergillus terreus H768 (Aspergillus terreus H768) fermented and extracted aspergillus fermented compounds in the preparation of antiallergic drugs. Background technique [0002] Food allergy, that is, food allergy, refers to the strong reaction of a certain tissue, a certain organ or even the whole body caused by some people after eating a certain food, resulting in various dysfunction or tissue damage, serious Or it may even cause anaphylactic shock. Food allergy is a threat to human life and health, and its incidence has gradually increased in the past few decades. Food allergic reactions are mainly mediated by serum immunoglobulin E (IgE), and this allergic reaction is usually accompanied by an increase in the content of specific immunoglobulin IgE. [0003] The current treatment for food allergy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/04A61K31/365A61P37/08C12R1/66
CPCA61K31/365C12P17/04C12N1/145C12R2001/66
Inventor 杨献文谢春兰夏金梅林伟翔汤熙翔邢翠平樊少强
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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