This invention discloses a method and
system for multi-stage
processing and reverse transcription stabilization of deep-sea in-situ metatranscriptomic
RNA, relating to the fields of deep-sea
biotechnology and marine
instrumentation. Within a continuous, sealed fluid channel, the following components are connected in series: a
filtration and enrichment chamber, a controlled decompression unit, a programmable
control unit, and at least one multifunctional
reaction chamber. The multifunctional
reaction chamber integrates a
temperature control system. At least one set of bidirectional opposing electromagnets is disposed on the outer wall of the multifunctional
reaction chamber. The programmable
control unit controls the on / off state and current of the bidirectional opposing electromagnets, generating a dynamically switchable spatial
magnetic field gradient within the multifunctional reaction chamber. This drives magnetic microspheres within the chamber to perform at least one of the following operations without
centrifugation: adsorption and aggregation, suspension and mixing, in-situ washing, inter-chamber transfer, and
elution /
desorption. All biochemical operations are completed within a continuous, sealed fluid channel, eliminating cross-
contamination between the sample and the external environment and achieving
fully automated sample processing in deep-sea environments.