Perfluorochemical molecular imprinting fluorescent probe and use method and application thereof
A technology of perfluorinated compounds and fluorescent probes, applied in the field of analytical chemistry, to achieve the effects of high detection sensitivity and specific selectivity, strong anti-interference, and accurate detection results
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Embodiment 1
[0040] (1) Weigh 3.0 g of citric acid, add 33 mL of deionized water, and stir magnetically for 5 minutes to dissolve all the solids. Add 1.0 mL of ethylenediamine (in a fume hood), and stir magnetically for 10 min. The solution was transferred to a hydrothermal reaction kettle, tightened and placed in an oven to react at 160°C for 4h. The dialysis bag with a cut-off of 300D was dialyzed for 48 hours, and the water was changed continuously during this period. The dialysate was freeze-dried in a glass petri dish to obtain a brown-yellow viscous carbon quantum dot solid.
[0041](2) Add 1% acetic acid to 0.3g chitosan for swelling; add template molecule PFOS 1mg. Stir at room temperature for 3 hours; add 1 mL of an aqueous solution containing 50 μg of carbon quantum dots, and 100 μL of epichlorohydrin, stir evenly, and then carry out crosslinking at a constant temperature of 40° C. for 4 hours. Afterwards, 20 g of chitosan molecularly imprinted fluorescent material with a wet ...
Embodiment 2
[0044] (1) The preparation process of carbon quantum dots is the same as in Example 1, except that 3.5 g of citric acid is used, 2.0 mL of ethylenediamine is used, the reaction temperature is 170° C., and the reaction time is 5 h. The cut-off capacity of the dialysis bag is 400D.
[0045] (2) Add 2% acetic acid to 0.5g chitosan for swelling; add 3 mg template molecule PFOS. Stir at room temperature for 3 hours; add 1 mL of an aqueous solution containing 100 μg of carbon quantum dots, 200 μL of epichlorohydrin, stir evenly, and then carry out crosslinking at a constant temperature of 40° C. for 8 hours. Afterwards, 20 g of chitosan molecularly imprinted fluorescent material with a wet weight was taken, and 45 mL of a mixed solution of acetone and 0.5 M sodium hydroxide with a volume ratio of 1:2 was used to elute at room temperature for 8 h under stirring conditions.
[0046] In step (2), the mass ratio of chitosan to perfluorinated compound template molecules is 166.67:1; the...
Embodiment 3
[0048] (1) The preparation process of carbon quantum dots is the same as in Example 1, except that 4.0 g of citric acid is used, 1.5 mL of ethylenediamine is used, the reaction temperature is 180° C., and the reaction time is 6 h. The cut-off capacity of the dialysis bag is 500D.
[0049] (2) Add 3% acetic acid to 0.8g chitosan to swell, add 5mg template molecule PFOS, stir at room temperature for 3h; add 1mL aqueous solution containing 200μg carbon quantum dots, 300μL epichlorohydrin stir evenly and then keep the temperature at 40℃ Cross-linking was carried out for 12h. Finally, 20 g of chitosan molecularly imprinted fluorescent material with a wet weight was taken, and 60 mL of a mixed solution of acetone and 0.5 M sodium hydroxide with a volume ratio of 1:3 was used to elute at room temperature for 12 h under stirring conditions.
[0050] In step (2), the mass ratio of chitosan to perfluorinated compound template molecules is 160:1; the mass ratio of chitosan, amino carbon...
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