A kind of perfluorinated compound molecularly imprinted fluorescent probe and its use method and application
A technology of perfluorinated compounds and fluorescent probes is applied in the field of analytical chemistry to achieve the effects of convenient analysis and detection, fast and simple detection, and reduction of errors
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Embodiment 1
[0041] (1) Weigh 3.0 g of citric acid, add 33 mL of deionized water, and stir magnetically for 5 minutes to dissolve all the solids. Add 1.0 mL of ethylenediamine (in a fume hood), and stir magnetically for 10 min. The solution was transferred to a hydrothermal reaction kettle, tightened and placed in an oven to react at 160°C for 4h. The dialysis bag with a cut-off of 300D was dialyzed for 48 hours, and the water was changed continuously during this period. The dialysate was freeze-dried in a glass petri dish to obtain a brown-yellow viscous carbon quantum dot solid.
[0042](2) Add 1% acetic acid to 0.3g chitosan for swelling; add template molecule PFOS 1mg. Stir at room temperature for 3 hours; add 1 mL of an aqueous solution containing 50 μg of carbon quantum dots, and 100 μL of epichlorohydrin, stir evenly, and then carry out crosslinking at a constant temperature of 40° C. for 4 hours. Afterwards, 20 g of chitosan molecularly imprinted fluorescent material with a wet ...
Embodiment 2
[0045] (1) The preparation process of carbon quantum dots is the same as in Example 1, except that 3.5 g of citric acid is used, 2.0 mL of ethylenediamine is used, the reaction temperature is 170° C., and the reaction time is 5 h. The cut-off capacity of the dialysis bag is 400D.
[0046] (2) Add 2% acetic acid to 0.5g chitosan for swelling; add 3 mg template molecule PFOS. Stir at room temperature for 3 hours; add 1 mL of an aqueous solution containing 100 μg of carbon quantum dots, 200 μL of epichlorohydrin, stir evenly, and then carry out crosslinking at a constant temperature of 40° C. for 8 hours. Afterwards, 20 g of chitosan molecularly imprinted fluorescent material with a wet weight was taken, and 45 mL of a mixed solution of acetone and 0.5 M sodium hydroxide with a volume ratio of 1:2 was used to elute at room temperature for 8 h under stirring conditions.
[0047] In step (2), the mass ratio of chitosan to perfluorinated compound template molecules is 166.67:1; the...
Embodiment 3
[0049] (1) The preparation process of carbon quantum dots is the same as in Example 1, except that 4.0 g of citric acid is used, 1.5 mL of ethylenediamine is used, the reaction temperature is 180° C., and the reaction time is 6 h. The cut-off capacity of the dialysis bag is 500D.
[0050] (2) Add 3% acetic acid to 0.8g chitosan to swell, add 5mg template molecule PFOS, stir at room temperature for 3h; add 1mL aqueous solution containing 200μg carbon quantum dots, 300μL epichlorohydrin stir evenly and then keep the temperature at 40℃ Cross-linking was carried out for 12h. Finally, 20 g of chitosan molecularly imprinted fluorescent material with a wet weight was taken, and 60 mL of a mixed solution of acetone and 0.5 M sodium hydroxide with a volume ratio of 1:3 was used to elute at room temperature for 12 h under stirring conditions.
[0051] In step (2), the mass ratio of chitosan to perfluorinated compound template molecules is 160:1; the mass ratio of chitosan, amino carbon...
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