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Stem cell preserving fluid used for transplantation of mesenchymal stem cells

A technology of bone marrow mesenchyme and stem cells, applied in the field of biomedicine, can solve the problems of increasing cell pollution and destroying cell vitality.

Pending Publication Date: 2018-04-17
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This process increases the possibility of cellular contamination while also compromising cell viability

Method used

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  • Stem cell preserving fluid used for transplantation of mesenchymal stem cells
  • Stem cell preserving fluid used for transplantation of mesenchymal stem cells
  • Stem cell preserving fluid used for transplantation of mesenchymal stem cells

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Embodiment approach

[0039] According to a preferred embodiment of the present invention, the stem cell preservation solution further includes epigallocatechin gallate (EGCG).

[0040] In a further preferred embodiment, the dosage of the epigallocatechin gallate (EGCG) is 1-20 mg / mL.

[0041] In a further preferred embodiment, the dosage of the epigallocatechin gallate (EGCG) is 5-10 mg / mL.

[0042] Among them, epigallocatechin gallate (EGCG) is the most effective antioxidant polyphenol compound in green tea extract, and its reducing ability can even reach 100 times that of L-isocorbic acid. Therefore, the application of EGCG to stem cells In the preservation solution, stem cells can be effectively prevented from being oxidized to die, and the survival rate of stem cells during preservation is greatly improved.

[0043] According to a preferred embodiment of the present invention, the stem cell preservation solution further includes a non-permeable cryoprotectant.

[0044] In a further preferred e...

Embodiment 1

[0065] Dissolve 6 mmol / L potassium dihydrogen phosphate in 10 mL of normal saline to prepare a 600 mmol / L potassium dihydrogen phosphate solution, and store it at 4°C after high-temperature sterilization.

[0066] Dissolve 5.35mg of adenosine in 2mL of normal saline, sterilize with a 0.22μ filter, and store at 4°C.

[0067] Dilute and dissolve potassium dihydrogen phosphate solution, low molecular weight heparin sodium and adenosine respectively with plasma to prepare the following stem cell preservation solution: containing plasma, 500-600 U / mL low molecular weight heparin sodium, 50-60 mmol / L dipotassium hydrogen phosphate and 0.5 μmol / L adenosine. The solution was stored at 4°C.

Embodiment 2

[0069] Dissolve 6 mmol / L potassium dihydrogen phosphate in 10 mL of normal saline to prepare a 600 mmol / L potassium dihydrogen phosphate solution, and store it at 4°C after high-temperature sterilization.

[0070] Dissolve 5.35mg of adenosine in 2mL of normal saline, sterilize with a 0.22μ filter, and store at 4°C.

[0071] Dilute and dissolve potassium dihydrogen phosphate solution, low molecular weight heparin sodium and adenosine respectively with plasma to prepare the following stem cell preservation solution: containing plasma, 500-600 U / mL low molecular weight heparin sodium, 50-60 mmol / L dipotassium hydrogen phosphate and 2 μmol / L adenosine. The solution was stored at 4°C.

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Abstract

The invention discloses a stem cell preserving fluid used for transplantation of mesenchymal stem cells. The stem cell preserving fluid contains plasma, low-molecular-weight heparin sodium, a dipotassium phosphate solution and adenosine, wherein the plasma contains plenty of albumin, which can provide nutrition support required by cells, and appropriate osmotic pressure and suitable electrolyte and buffer pair can maintain normal activity of cells; low-molecular-weight heparin sodium can inhibit adhesion of mesenchymal stem cells; the dipotassium phosphate solution can provide steady externalenvironment support for cells; and adenosine can promote the self-repairing capability of cells, exert a protective effect and maintain the cell reproductive capacity. Therefore, the stem cell preserving fluid adopts plasma, low-molecular-weight heparin sodium, dipotassium phosphate solution and adenosine as main components, so that the mesenchymal stem cells keep relatively high activity in an in-vitro state, loss caused by the fact that cells are not delivered back to the body in time is reduced, adhesion and clustering of cells is avoided, and relatively high multiplication capacity can beensured after the cells are reheated.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a cell preservation solution, in particular to a stem cell preservation solution, in particular to a bone marrow mesenchymal stem cell preservation solution. Background technique [0002] Stem cells are a type of cells with self-renewal and multi-directional differentiation potential, and have the ability to continuously reproduce themselves. At present, stem cell research mainly focuses on adult stem cells, embryonic stem cells, and induced pluripotent stem cells. Mesenchymal stem cells are a kind of mesoderm-derived stem cells with multidirectional differentiation ability, which widely exist in various tissues of the body. Because of its extremely low immunogenicity and strong immune regulation ability, mesenchymal stem cells have very broad clinical application prospects in the fields of hematopoietic reconstruction, immunotherapy and tissue repair. Among them, bone marrow mesenchyma...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 陈永平陈达之萧云蕾许烂漫李骥卢明芹金晓芝
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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