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Method for testing gelatin sample by adopting infrared spectroscopy

A technology of infrared spectroscopy and gelatin, which is applied to measurement devices, material analysis by optical means, instruments, etc., can solve the problems of influence of test results, high price, erosion, etc., and achieve accurate and reliable infrared test results, and the preparation method is simple and easy. The effect of grinding

Inactive Publication Date: 2018-04-17
BAOTOU DONGBAO BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The window used in this method is made of a whole piece of transparent potassium bromide (or sodium chloride) crystal, which is difficult to make and expensive. Corrosion, limited life
Gelatin samples are only soluble in water but not in organic solvents, and moisture is a large interference item in infrared testing, and miscellaneous peaks will appear, and if there is O-H in the sample itself (the group structure of water is O-H), it will be harmful Test results are affected, so gelatin samples are not suitable for this method of preparation
[0005] ③Hot pressing film method (polymer sample): This method is only for samples that can be hot-melted, but samples that cannot be hot-melted cannot be prepared by this method
[0006] ④ATR method: The test principle of this method is different from the traditional infrared test. It can only detect the surface structure of the substance, and is suitable for samples with uniform structure; however, the structure of gelatin is not uniform, and it is difficult to achieve accurate detection
The moisture interference in the infrared spectrum obtained by this method is too large, and it is impossible to obtain an accurate infrared spectrum of gelatin

Method used

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  • Method for testing gelatin sample by adopting infrared spectroscopy
  • Method for testing gelatin sample by adopting infrared spectroscopy
  • Method for testing gelatin sample by adopting infrared spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040]Mix 0.05 g of gelatin particles with 99.95 g of water at 20° C., swell for 1 hour, and dissolve in a 60° C. water bath for 30 minutes to obtain a glue solution with a gelatin concentration of 0.05 wt %. The glue solution was placed in a centrifuge tube, pre-frozen at -20°C for 3 hours, and then freeze-dried at -80°C for 24 hours to obtain a freeze-dried powder, which was stored in a desiccator. Potassium bromide was dried at 105°C for 4 hours and stored in a desiccator. Mix and grind 1 mg of freeze-dried powder and 200 mg of potassium bromide to obtain a uniform mixed powder. Put the mixed powder in a mold, press it on a hydraulic press with a pressure of 50 MPa for 60 seconds, and release the pressure to obtain a light-transmitting sheet. Conduct an infrared test, and the result is see figure 1 .

Embodiment 2

[0042] Mix 0.5 g of gelatin particles with 99.5 g of water at 15° C., swell for 2 hours, and dissolve in a 60° C. water bath for 40 minutes to obtain a gelatin solution with a gelatin concentration of 0.5 wt %. The glue solution was placed in a centrifuge tube, pre-frozen at -25°C for 2 hours, and then freeze-dried at -70°C for 30 hours to obtain a freeze-dried powder, which was stored in a desiccator. Potassium bromide was dried at 105°C for 4 hours and stored in a desiccator. Mix and grind 2 mg of freeze-dried powder and 200 mg of potassium bromide to obtain a uniform mixed powder, place the mixed powder in a mold, press on a hydraulic press with a pressure of 100 MPa for 45 seconds, and release the pressure to obtain a light-transmitting sheet for infrared testing.

Embodiment 3

[0044] Mix 1 g of gelatin particles with 99 g of water at 20° C., swell for 1 hour, and dissolve in a 60° C. water bath for 30 minutes to obtain a gelatin solution with a gelatin concentration of 1 wt%. The glue solution was placed in a centrifuge tube, pre-frozen at -20°C for 3 hours, and then freeze-dried at -80°C for 24 hours to obtain a freeze-dried powder, which was stored in a desiccator. Potassium bromide was dried at 105°C for 4 hours and stored in a desiccator. Mix and grind 2 mg of freeze-dried powder and 200 mg of potassium bromide to obtain a uniform mixed powder, place the mixed powder in a mold, press on a hydraulic press with a pressure of 50 MPa for 60 seconds, and release the pressure to obtain a light-transmitting sheet for infrared testing.

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Abstract

The invention discloses a method for testing a gelatin sample by adopting infrared spectroscopy. The method comprises a gelatin sample treating process, the treating process comprises the following steps: (1) adding water into gelatin and dissolving to obtain a glue solution with the gelatin concentration of 0.01-2wt%; (2) carrying out freeze drying treatment on the glue solution to obtain freeze-dried powder; and (3) mixing the freeze-dried powder with potassium bromide, and grinding, so that uniform mixed powder is obtained; and tabletting the mixed powder, so that light-transmitting sheetsare obtained. The method disclosed by the invention is simple to operate, and an accurate infrared test result can be obtained.

Description

technical field [0001] The invention relates to a method for testing gelatin samples using infrared spectroscopy. Background technique [0002] At present, there are mainly four methods for the preparation of infrared test samples: [0003] ① Solid tableting method (solid particle sample): The fine powder of the solid sample is evenly dispersed in the alkali metal halide and pressed into a transparent sheet. Potassium bromide is generally used as the substrate, and 1-2 mg of sample and 200 mg of pure KBr are ground evenly, placed in a mold, and used (5-10)×10 7 Pa pressure is pressed into a transparent sheet on a hydraulic press. Gelatin is a complex mixture of macromolecules. Its particles are hard and difficult to grind. Therefore, it is not effective to mix with potassium bromide for uniform sample preparation and solid compression. [0004] ②Coating film, thin film, liquid film method (liquid sample): Take an appropriate amount of liquid sample and apply it evenly on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3563
CPCG01N21/3563G01N2021/3572
Inventor 康虹王富荣钟伟王冉张亚楠胡晨雪李春娥杨瑞婷吕晓东张煜申磊关志强
Owner BAOTOU DONGBAO BIO TECH CO LTD
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