Culture method for selenium-rich dendrobium officinale kimura ex migo with high transplanting survival rate
A technology of transplanting survival rate and Dendrobium officinale, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of restricting the production scale of selenium-enriched Dendrobium officinale, difficult to guarantee the quality of tissue culture seedlings, and demanding growth environment , to achieve the effect of expanded production scale, stable quality and broad market prospects
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Embodiment 1
[0026] A method for cultivating selenium-enriched dendrobium officinale seedlings with high transplanting survival rate, the steps are as follows:
[0027] 1) Take the stem section of Dendrobium candidum, soak it in 90% alcohol for 1 minute, take it out, rinse it twice with sterile water, then soak it in 0.1% mercuric chloride solution for 5 minutes, and then rinse it four times with sterile water, Obtain the stem section of aseptic dendrobium officinale;
[0028] 2) Cutting the sterile Dendrobium officinale stem section into thin slices along the cross section to obtain tissue slices, the thickness of the tissue slices being 1mm;
[0029] 3) Put the tissue slices into the induction medium, and germinate the protocorms at 22°C without light to obtain the protocorms. The induction medium is 1 / 2MS medium with 5g / L peptone, 8g / L licorice extract, 0.4mg / L sodium selenite, mix evenly, and sterilize at 110°C for 20min.
[0030] 4) Inoculate the protocorms in the proliferation medi...
Embodiment 2
[0036] A method for cultivating selenium-enriched dendrobium officinale seedlings with high transplanting survival rate, the steps are as follows:
[0037] 1) Take the stem section of Dendrobium officinale, soak it in 90% alcohol for 1 minute, take it out, rinse it with sterile water for 3 times, then soak it in 0.1% mercuric chloride solution for 6 minutes, and then rinse it with sterile water for 4 times, Obtain the stem section of aseptic dendrobium officinale;
[0038] 2) Cutting the sterile Dendrobium officinale stem section into thin slices along the cross section to obtain tissue slices, the thickness of the tissue slices is 1.5 mm;
[0039]3) Put the tissue slices into the induction medium, and germinate the protocorms at 23°C without light to obtain the protocorms. The induction medium is 1 / 2MS medium with 5g / L peptone, 8g / L licorice extract, 0.4mg / L sodium selenite, mix evenly, and sterilize at 110°C for 20min.
[0040] 4) Inoculate the protocorms in the proliferat...
Embodiment 3
[0046] A method for cultivating selenium-enriched dendrobium officinale seedlings with high transplanting survival rate, the steps are as follows:
[0047] 1) Take the stem section of Dendrobium officinale, soak it in 90% alcohol for 1.5 minutes, take it out, rinse it with sterile water for 3 times, then soak it in 0.1% mercuric chloride solution for 7 minutes, and rinse it with sterile water for 4 times , to obtain aseptic stems of Dendrobium officinale;
[0048] 2) Cutting the sterile Dendrobium officinale stem section into thin slices along the cross section to obtain tissue slices, the thickness of the tissue slices is 1.5mm;
[0049] 3) Put the tissue slices into the induction medium, and germinate protocorms at 24°C without light to obtain protocorms. The induction medium is 1 / 2 MS medium with 5g / L peptone, 8g / L licorice extract, 0.4mg / L sodium selenite, mix evenly, and sterilize at 110°C for 20min.
[0050] 4) Inoculate the protocorms in the proliferation medium, and ...
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