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A technique of tissue culture and wild mugwort, which is applied in the field of plant tissue culture, can solve the problems of endangered species and the reduction of wild mugwort
Active Publication Date: 2018-04-20
CHANGSHA UNIVERSITY
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[0004] In order to solve the long-term over-harvesting of a large number of wild mugwort, which leads to the rapid decline of wild mugwort, and even the problem of extinction, as well as to protect the wild resources of wild mugwort and meet people's needs, select excellent Hunan wild mugwort varieties and apply tissue culture Rapid mas...
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Embodiment 1
[0027] A method for tissue culture of Hunan wild mugwort, comprising the steps of:
[0028] 1) Formation of callus on leaves: Disinfect the leaves of Artemisia argyi in the wild, cut off the veins and leaf margins, cut into small pieces of about 5mm×5mm, inoculate them on the callus induction medium, and culture them conventionally to callus formation. The induction medium of the leaf callus formation is the MS medium comprising 0.5mg / L 6-benzylaminopurine and 0.1mg / L naphthaleneacetic acid; the time for the leaf callus formation is 20d; the MS The concentration of sucrose in the medium is 30g / L, and the concentration of agar powder is 9g / L; the culture conditions of the conventional culture are that the pH value is 6.0, the light intensity is 2000lx, the light time is 16h / d, and the temperature of the constant temperature incubator is 20-25 ℃.
[0029] 2) Differentiation of callus into adventitious buds: the callus formed in step 1) was cut into small pieces of about 5 mm×5...
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Abstract
The invention relates to the field of tissue culture of plants, and discloses a tissue culture method for Hunan artemisia umbrosa. The tissue culture method comprises the following steps: firstly, forming calluses by leaf blades: carrying out sterilizing treatment on field-picked Hunan artemisia umbrosa leaf blades, then cutting off leaf veins and leaf margins, cutting into small blocks with the area of about 5mm x 5mm, inoculating the small blocks to a callus-induced culture medium, and carrying out conventional culture to form the calluses; secondly, differentiating adventitious buds by thecalluses: cutting the calluses formed in the first step into the small blocks with the area of 5mm*5mm, inoculating the small blocks to an induced culture medium of the adventitious buds, and carryingout conventional culture until the adventitious buds are formed on the calluses; thirdly, rooting the adventitious buds: cutting off the adventitious buds with the size of about 3 to 5cm, formed in the second step, inoculating the adventitious buds into a rooting induced culture medium, and carrying out conventional culture until roots are formed, thus obtaining artemisia umbrosa tissue culture seedlings. By use of the method disclosed by the invention, a large amount of high-quality artemisia umbrosa tissue culture seedlings can be obtained within 47 days; a technological base is laid for realizing protection for wild resources of artemisia vulgaris, the demand of cultivation on the seedlings and establishing of a gene bank.
Description
technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for tissue culture of Hunan wild mugwort. Background technique [0002] Wild mugwort (Artemisia lavandulaefolia DC.) is a perennial herb of the genus Artemisia in the family Asteraceae, sometimes in the form of a semi-shrub, with a fragrant plant; the main root is slightly obvious, and there are many lateral roots; the rhizome is slightly thick, with a diameter of 4-6mm, often crawling, Thin and short vegetative branches; a few stems, in small clusters, rare solitary, 50-120cm high; leaves papery, green above, with dense white glandular spots and small pits; capitulum extremely numerous, oval or Oblong, 2-2.5mm in diameter; achenes long ovate or obovate; flowers and fruits in August-October. Produced in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Hebei, Shanxi, Shaanxi, Gansu, Shandong, Jiangsu, Anhui, Jiangxi, Henan, Hubei, Hunan, Guangdong (northern), Guangxi (n...
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