Dicofol residue rapid detection method and kit thereof

A detection kit, the technology of dicofol, is applied in the direction of material analysis by observing the impact on chemical indicators, and analysis by chemical reaction of materials, etc., which can solve the problems of long operation time, weak reactivity and low accuracy. , to achieve the effect of improving color rendering effect, accurate experimental results and high sensitivity

Active Publication Date: 2018-04-20
GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD +1
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Problems solved by technology

Most of the existing rapid detection methods for dicofol use the pyridine-alkaline colorimetric method, and pyridine is a volatile and odorous toxic solvent, which will cause certain harm to the human body
At the same time, the accuracy of this method is not high

Method used

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  • Dicofol residue rapid detection method and kit thereof

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[0042] Example:

[0043] Steps

[0044] 1) Extraction-extraction: Weigh 2.5g of sample into a centrifuge tube, add 10mL of n-hexane, shake up and down for 30s, draw the extract through a solid-phase extraction column, blow dry, and elute the solid-phase column with 1mL of acetonitrile;

[0045] 2) Reaction color development: add 0.3 g of 2,2-bipyridine, add 30 μL of sodium hydroxide solution and 50 μL of tetramethylammonium hydroxide solution, and heat in a water bath at 80-100° C. for 2 min. If it is yellow, it indicates that it contains dicofol.

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Abstract

The invention discloses a dicofol residue rapid detection method and a kit thereof. The dicofol residue rapid detection method comprises the following steps: extracting a sample, extracting a solid phase, after extracting a sample extract through a solid-phase extraction column, eluting the sample extract by an eluate, adding 2,2-dipyridine, a strong alkaline solution and tetramethylammonium hydroxide in the eluted eluate, and performing a chromogenic reaction and colorimetric determination to obtain the dicofol amount. According to the invention, sample detection can be completed by only 20 min, dipyridine having the characteristics of odorlessness and safety is taken as a reaction developer, and an experiment method realizes green environmental protection; tetramethylammonium hydroxide is added as a reaction catalyst, so that the chromogenic reaction can be completed in two minutes, and the chromogenic effect is enhanced; the method determines the dicofol content by comparing the colors, sensitivity is high, and the method can be widely used for rapidly detecting dicofol in samples such as vegetables, fruits, and tea.

Description

technical field [0001] The invention relates to a detection method and a kit for pesticide residues, in particular to a rapid detection method and kit for dicofol based on a color reaction. Background technique [0002] Dicofol is a broad-spectrum, low-toxicity organochlorine acaricide, which is mainly used to control various mites in cotton, fruit trees, flowers, etc. Because dicofol is not easy to decompose and has high residues, it is not suitable for tea, vegetables and edible fungi. However, the use of dicofol is still common in my country at present, and the detection rate of dicofol in tea, vegetables and edible fungi is relatively high. [0003] Dicofol will undergo an alkaline hydrolysis reaction under alkaline conditions to produce chloroform and other substances. Chloroform will react in the pyridine-NaOH solution system to form a red compound. According to the absorbance value of the red compound, the concentration of dicofol can be calculated. Most of the exi...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 吴沛堂谢俊平梁科卢新石松温林凤
Owner GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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