Rapid identification method of combined resistance to watermelon and melon wilt and gummy stem blight in seedling stage

An identification method and fusarium wilt technology, applied in botany equipment and methods, biochemical equipment and methods, horticultural methods, etc., can solve the problems of plant death, interactive effects of disease onset, high disease index, etc., to shorten the cycle and save seeds , the effect of improving identification efficiency

Active Publication Date: 2018-04-27
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The single inoculation method is inefficient, consumes a lot of test materials such as seeds, and the tests of different batches are unstable
There are also a small number of reports on combined inoculation of fusarium wilt and wilt, that is, live organisms are inoculated successively with fusarium wilt and wilt. The current combined inoculation method in...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid identification method of combined resistance to watermelon and melon wilt and gummy stem blight in seedling stage
  • Rapid identification method of combined resistance to watermelon and melon wilt and gummy stem blight in seedling stage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0040] A rapid identification method for joint resistance to melon wilt and vine blight at seedling stage, comprising the following steps:

[0041] (1) Preparation of test plant seedlings: take Kahong (resistant to fusarium wilt, susceptible to wilt), Sumi No. 1 (susceptible to fusarium wilt, susceptible to wilt), W7 (susceptible to fusarium wilt, moderately resistant to wilt) Soak watermelon seeds in 10% sodium hypochlorite solution for 5 minutes, rinse with running water to remove the residual disinfectant on the surface, then soak the seeds in warm water at 50-60°C, stir to room temperature, and place them for 4 hours, then take them out for disinfection and sow. After the seedlings grow to 3-5 true leaves, set aside;

[0042] (2) Preparation of pathogenic bacteria:

[0043] Fusarium wilt pathogen preparation: Inoculate watermelon Fusarium wilt bacteria on PDA solid medium, culture at 26-28°C for one week, then inoculate the cultured Fusarium wilt bacteria on PDB liquid me...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a rapid identification method of combined resistance to watermelon and melon wilt and gummy stem blight in the seedling stage. The method comprises immersing watermelon and melon seeds to be identified in warm water, carrying out disinfection and sowing, after the seedling has 3-5 main leaves, taking leaves, carrying out spray-inoculation with a didymella bryoniae spore suspension, washing roots of the seedling through water, dipping the roots in the didymella bryoniae spore suspension, transferring the seedling into a container with a matrix, carrying out culture at the room temperature, observing the disease cases at 3-7 days after inoculation, surveying cases of the watermelon and melon wilt and gummy stem blight, carrying out statistics on the morbidity of wiltand the morbidity and disease index of gummy stem blight, and carrying out classification prediction on the watermelon and melon wilt and gummy stem blight in the seedling stage according to the morbidity and disease index. The method can fast identify the watermelon and melon seed resources with wilt and gummy stem blight resistance based on the same type of the same plant, improve the identification efficiency and shorten the cycle and save test materials such as seeds and is suitable for fast screening of a large number of watermelon and melon antigens.

Description

technical field [0001] The invention relates to a rapid identification method for joint resistance to melon wilt and vine blight at the seedling stage, and belongs to the technical field of plant germplasm identification. Background technique [0002] Fusarium oxysporum (Fusarium oxysporum) infection caused by Fusarium wilt and Ascochytacitrullina Smith (its asexual generation is Didymella bryoniae) caused by Ascochytacitrullina Smith are two major fungi in melon crops. Sexual diseases can occur in winter and spring solar greenhouses and greenhouses in early spring and autumn from the seedling stage to the harvest stage. In production, the plant mortality rate can often reach 30% to 40%, resulting in severe production reduction. It has become a constraint on the production of melons. main obstacle. Breeding new varieties of high-quality and compound disease-resistant melons is the main method to effectively control melon wilt and creeping blight. Therefore, popularizing the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/18A01G7/06C12R1/77C12R1/645
CPCA01G7/06C12Q1/18G01N2333/37
Inventor 姚协丰羊杏平徐锦华张曼任润生刘广
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap