Rapid identification method of combined resistance to watermelon and melon wilt and gummy stem blight in seedling stage

Abstract

The invention relates to a rapid identification method of combined resistance to watermelon and melon wilt and gummy stem blight in the seedling stage. The method comprises immersing watermelon and melon seeds to be identified in warm water, carrying out disinfection and sowing, after the seedling has 3-5 main leaves, taking leaves, carrying out spray-inoculation with a didymella bryoniae spore suspension, washing roots of the seedling through water, dipping the roots in the didymella bryoniae spore suspension, transferring the seedling into a container with a matrix, carrying out culture at the room temperature, observing the disease cases at 3-7 days after inoculation, surveying cases of the watermelon and melon wilt and gummy stem blight, carrying out statistics on the morbidity of wiltand the morbidity and disease index of gummy stem blight, and carrying out classification prediction on the watermelon and melon wilt and gummy stem blight in the seedling stage according to the morbidity and disease index. The method can fast identify the watermelon and melon seed resources with wilt and gummy stem blight resistance based on the same type of the same plant, improve the identification efficiency and shorten the cycle and save test materials such as seeds and is suitable for fast screening of a large number of watermelon and melon antigens.

Description

technical field [0001] The invention relates to a rapid identification method for joint resistance to melon wilt and vine blight at the seedling stage, and belongs to the technical field of plant germplasm identification. Background technique [0002] Fusarium oxysporum (Fusarium oxysporum) infection caused by Fusarium wilt and Ascochytacitrullina Smith (its asexual generation is Didymella bryoniae) caused by Ascochytacitrullina Smith are two major fungi in melon crops. Sexual diseases can occur in winter and spring solar greenhouses and greenhouses in early spring and autumn from the seedling stage to the harvest stage. In production, the plant mortality rate can often reach 30% to 40%, resulting in severe production reduction. It has become a constraint on the production of melons. main obstacle. Breeding new varieties of high-quality and compound disease-resistant melons is the main method to effectively control melon wilt and creeping blight. Therefore, popularizing the...

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Problems solved by technology

The single inoculation method is inefficient, consumes a lot of test materials such as seeds, and the tests of different batches are unstable

There are also a small number of reports on combined inoculation of fusarium wilt and wilt, that is, live organisms are inoculated successively with fusarium wilt and wilt. The current combined inoculation method inoculates two pathogenic bacteria at the same time, often resulting in interactive effects of disease onset, high disease index, and prone to errors As a result, and because the Fusarium wilt is fatal to the plants, the plants basically all died after inoculation, and the multi-resistance seedling identification test could not be carried out. vaccination test

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