Modeling method for new blood vessels in abdominal aorta atherosclerotic plaques of New Zealand white rabbit

Abstract

The invention provides a modeling method for new blood vessels in abdominal aorta atherosclerotic plaques of a New Zealand white rabbit, which comprises the following steps: at nearby positions whereplaques need injection in an abdominal aorta of the New Zealand white rabbit fed by balloon injury plus high cholesterol for 12 weeks, under ultrasonic guidance in the blood vessel, a pair of fine forceps are used for accurate positioning by pressing a vascular lumen at a vascular adventitia to cause local deformation and removing pressure to restore the shape of the lumen; FGF-2, VEGF-A or mixedlentiviruses of both are injected into the plaques; plaque-injected sites are located by both an iliopsoas suture and the distance of iliac arterial bifurcation; and the New Zealand white rabbit continues to be fed by high fat for 8 weeks. The modeling method can effectively construct plaques which are rich in new blood vessels, and a modeling success rate reaches 100 percent. A pathological section and H&E chemical staining prove that the rich new blood vessels can be seen in the plaques no matter which of VEGF-A, FGF-2 or a VEGF-A+FGF-2 mixture is injected, even the new blood vessels alreadygrowing into fibrous caps of the plaques (figure 6) can be seen, and in comparison with a normal saline-injected control group, the difference reaches statistical significance (P<0.05).

Description

technical field [0001] The invention belongs to the field of atherosclerotic plaque modeling, in particular to a method for modeling new blood vessels in abdominal aortic atherosclerotic plaque of New Zealand white rabbits. Background technique [0002] Cardiovascular and cerebrovascular diseases are the number one killer of human health, and have become the main cause of death and disability in middle-aged and elderly people. The rupture of unstable plaques and subsequent thrombosis are important reasons. Intra-plaque neovascularization is one of the important characteristics of plaque instability. Because the wall structure of the new blood vessels in the plaque is incomplete and only surrounded by a layer of endothelial cells, lacking the support of pericytes, connective tissue and basement membrane, red blood cells, inflammatory factors and inflammatory cells can penetrate into the plaque through the endothelial gap Intra-plaque hemorrhage and inflammatory response are ...

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Problems solved by technology

[0013] In order to overcome the above disadvantages, the present invention provides a method for modeling new blood vessels in New Zealand rabbit abdominal aortic atherosclerotic plaques, which overcomes the low film formation rate (generally about 50%) of the existing modeling methods and cannot Problems with the formation of new blood vessels within the plaque

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