Duck hepatitis A virus antigen-antibody complex vaccine and preparation method thereof
A duck hepatitis A virus, antigen-antibody technology, applied in the field of poultry vaccines, can solve the problems of immunity blank period and short protection period, and achieve the effects of long protection period, high protection rate and high titer
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Embodiment 1
[0024] 1. Materials
[0025] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.
[0026] 2. Method
[0027] Preparation of Type 2.13 Duck Hepatitis A Virus Antigen (Immunogen)
[0028] 1) Seed virus for production: Type 3 duck hepatitis A virus (SD15 strain) for seedling production, which is isolated, identified, kept and supplied by Anhui Qiangying Duck Industry Group Co., Ltd., biological deposit number: CGMCCNo.14739.
[0029] 2) SD15 strain venom preparation: Dilute SD15 strain 200 times with physiological saline (V / V), inoculate 11-day-old duck embryos in the allantoic cavity, 0.2ml per embryo, incubate at 37°C, and irradiate embryos every 4 hours Check it out once. The duck embryos that died within 24 to 96 hours after inoculation were collected, and placed at -20°C for 0.5 hours, then the al...
Embodiment 2
[0063] This embodiment provides a kind of preparation method of duck hepatitis A virus antigen-antibody complex vaccine, and the steps comprise:
[0064] (1) Use type 3 duck hepatitis A virus to immunize the allantois to inoculate duck embryos, incubate at 37°C, collect dead duck embryos within 24-96 hours after inoculation, freeze at -20°C for 1 hour, and collect allantoic fluid respectively and embryo body; remove the head and limbs of the embryo body, fully homogenate, centrifuge to get the supernatant and mix it with the allantoic fluid, filter through a 0.22 micron filter membrane, add 0.5% by mass magnesium sulfate, 0.25% by mass chlorine Sodium chloride and 0.5% propolis by mass are mixed evenly to obtain type 3 duck hepatitis A virus antigen, and other steps are the same as in Example 1. At the same time, the control group A was set without adding magnesium sulfate, sodium chloride and propolis to obtain the control group A antigen.
[0065] (2) Preparation of egg yol...
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