Duck hepatitis A virus antigen-antibody complex vaccine and preparation method thereof

A duck hepatitis A virus, antigen-antibody technology, applied in the field of poultry vaccines, can solve the problems of immunity blank period and short protection period, and achieve the effects of long protection period, high protection rate and high titer

Active Publication Date: 2018-05-04
ANHUI QIANGYING DUCK IND GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies in the prior art, a kind of preparation method of duck hepatitis A virus antigen-antibody complex vaccine is provided, to solve existing 3 types of duck hepatitis A virus vaccines and have immune blank period, short protection period etc. technical problem

Method used

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  • Duck hepatitis A virus antigen-antibody complex vaccine and preparation method thereof
  • Duck hepatitis A virus antigen-antibody complex vaccine and preparation method thereof
  • Duck hepatitis A virus antigen-antibody complex vaccine and preparation method thereof

Examples

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Embodiment 1

[0024] 1. Materials

[0025] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0026] 2. Method

[0027] Preparation of Type 2.13 Duck Hepatitis A Virus Antigen (Immunogen)

[0028] 1) Seed virus for production: Type 3 duck hepatitis A virus (SD15 strain) for seedling production, which is isolated, identified, kept and supplied by Anhui Qiangying Duck Industry Group Co., Ltd., biological deposit number: CGMCCNo.14739.

[0029] 2) SD15 strain venom preparation: Dilute SD15 strain 200 times with physiological saline (V / V), inoculate 11-day-old duck embryos in the allantoic cavity, 0.2ml per embryo, incubate at 37°C, and irradiate embryos every 4 hours Check it out once. The duck embryos that died within 24 to 96 hours after inoculation were collected, and placed at -20°C for 0.5 hours, then the al...

Embodiment 2

[0063] This embodiment provides a kind of preparation method of duck hepatitis A virus antigen-antibody complex vaccine, and the steps comprise:

[0064] (1) Use type 3 duck hepatitis A virus to immunize the allantois to inoculate duck embryos, incubate at 37°C, collect dead duck embryos within 24-96 hours after inoculation, freeze at -20°C for 1 hour, and collect allantoic fluid respectively and embryo body; remove the head and limbs of the embryo body, fully homogenate, centrifuge to get the supernatant and mix it with the allantoic fluid, filter through a 0.22 micron filter membrane, add 0.5% by mass magnesium sulfate, 0.25% by mass chlorine Sodium chloride and 0.5% propolis by mass are mixed evenly to obtain type 3 duck hepatitis A virus antigen, and other steps are the same as in Example 1. At the same time, the control group A was set without adding magnesium sulfate, sodium chloride and propolis to obtain the control group A antigen.

[0065] (2) Preparation of egg yol...

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Abstract

The invention discloses a duck hepatitis A virus antigen-antibody complex vaccine and a preparation method thereof. The vaccine is an antigen-antibody complex obtained after a 3 type duck hepatitis Avirus antigen and a duck egg yolk antibody are mixed and react in a volume ratio of 1:50. Compared with the prior art, the vaccine disclosed by the invention has the advantages that magnesium sulfate,sodium chloride and propolis are added into the 3 type duck hepatitis A virus antigen, and the antigen is used for immunizing laying ducks to prepare duck egg yolk antigens, and finally egg yolk antibodies and the 3 type duck hepatitis A virus antigen are reasonably matched, so that the 3 type duck hepatitis A virus antigen-antibody complex vaccine is prepared. Multiple tests show that binding efficiency of the obtained antigen-antibody complex takes effect after the vaccine is injected within 2 hours, no blank immunization period exists, titer is high, safety is good, protection rate is high, and protection period is long.

Description

technical field [0001] The invention relates to the technical field of poultry vaccines, in particular to a duck hepatitis A virus antigen-antibody complex vaccine and a preparation method thereof. Background technique [0002] Duck viral hepatitis A is an acute and highly fatal infectious disease caused by duck hepatitis A virus of the Picornaviridae family. It is currently one of the most important diseases that endanger ducklings. Duck viral hepatitis A is divided into three serotypes, namely: DHAV-1, DHAV-2 and DHAV-3. In recent years, the clinical isolation rate of duck hepatitis A virus type 3 is close to 90%, and it is the main serotype prevalent in my country at present. [0003] Existing reports have confirmed that duck viral hepatitis A can occur in ducks aged 2-39 days. At present, the disease is mainly prevented by vaccines or hyperimmune antibodies. There is an immune blank period in vaccine immunization prevention, and it cannot protect young ducks with disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K39/125A61K47/46A61P31/14
CPCA61K39/12A61K39/42A61K47/46A61K2039/505C12N2770/32334A61K2300/00
Inventor 郝东敏钟洪义鲁爱玲郑化鹏刘诗柱王善辉
Owner ANHUI QIANGYING DUCK IND GRP
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