A device for high-throughput and rapid preparation of micro-droplets and its application

Abstract

The invention discloses a high-throughput and rapid micro-droplet preparation device and an application thereof. The device comprises an array chip set, a temperature control module, a pressure control module, a displacement module, a vibration module and a control circuit, wherein the control circuit is connected with the vibration module, the pressure control module, the temperature control module and the displacement module; the displacement module is connected with the temperature control module and the pressure control module; the array chip set comprises a micro-tube array chip and a micro-cell array chip, an airtight cover in the pressure control module and a metal hole seat in the temperature control module are arranged at the top and the bottom of the micro-tube array chip respectively, and a movable part of a voice coil motor of the vibration module is connected with the micro-cell array module. The device has the following advantages: the structure is simple due to the absence of complicated pump valve pipes, the size is small and compact, the automation degree is high, the requirement for parallel generation of micro-droplets with different inclusions can be met, the use is flexible and the application range is wide. Therefore, the device can be used for high-throughput and rapid micro-droplet preparation.

Description

technical field [0001] The invention belongs to the field of microfluidic chip analysis, and in particular relates to a high-throughput and rapid micro-droplet preparation device and its application. Background technique [0002] Micro-droplet technology is a brand-new microfluidic technology developed in recent years to manipulate tiny volumes of liquid. Compared with the traditional continuous flow, the discrete droplets can be used as relatively independent microreactors or reservoirs, with minimal reagent consumption, which is conducive to reducing costs and achieving high throughput, and no diffusion between droplets and samples can avoid inter-sample It has the advantages of low cross-contamination, stable reaction conditions, easy manipulation, and large specific surface area that can accelerate heat / mass exchange. Therefore, it is an ideal tool for drug synthesis, single-cell analysis, molecular biology, and material synthesis. [0003] The preparation of micro-drop...

AI Technical Summary

Problems solved by technology

For example: gas quickly dissipates in commonly used microfluidic chip substrate materials (such as PDMS); electrokinetic methods require high voltage, which is not conducive to the application in biology; optical effects may cause changes in the contents of droplets (such as photobleaching) etc.

More importantly, these developed methods, including the hydrodynamic method, still have some shortcomings when preparing batches of heterogeneous samples: (1) the droplet generation control system is too large and complex; (2) the generated heterogeneous droplets require Subsequent cumbersome sorting operations; (3) limited parallel throughput or generation speed

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