High-throughput and rapid micro-droplet preparation device and application thereof

A micro-droplet, high-throughput technology, used in fluid controllers, laboratory containers, laboratory utensils, etc., can solve the problems of high voltage, large and complex control systems, dissipation and other problems in electrodynamic methods, and achieve automation High degree, wide application range, flexible use effect

Active Publication Date: 2018-05-04
GUANGZHOU FIRST PEOPLES HOSPITAL (GUANGZHOU DIGESTIVE DISEASE CENT GUANGZHOU FIRST PEOPLES HOSPITAL GUANGZHOU MEDICAL UNIV THE SECOND AFFILIATED HOSPITAL OF SOUTH CHINA UNIV OF TECH)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example: gas quickly dissipates in commonly used microfluidic chip substrate materials (such as PDMS); electrokinetic methods require high voltage, which is not conducive to the application in biology; optical effects may cause changes in the contents of droplets (such as photobleaching) etc.
More importantly, these developed methods, including the hydrodynamic method, still have some shortcomings when preparing batches of heterogeneous samples: (1) the droplet generation control system is too large and complex; (2) the generated heterogeneous droplets require Subsequent cumbersome sorting operations; (3) limited parallel throughput or generation speed

Method used

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  • High-throughput and rapid micro-droplet preparation device and application thereof
  • High-throughput and rapid micro-droplet preparation device and application thereof
  • High-throughput and rapid micro-droplet preparation device and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] like figure 1 As shown, a device for high-throughput and rapid preparation of micro-droplets includes an array chip set 1, a vibration module 2, a voltage control module 3, a temperature control module 4, a displacement module 5 and a control circuit 6; the control circuit 6 is connected to the The vibration module 2, the voltage control module 3, the temperature control module 4 and the displacement module 5 are connected; the displacement module is respectively connected with the temperature control module and the voltage control module.

[0054] like Figure 2.1 and 2.2 As shown, the array chip set 1 includes a microtube array chip 1-1 and a microwell array chip 1-2 arranged up and down in sequence, and are used to carry the dispersed phase liquid A and the continuous phase liquid B respectively. Among them, the microtube array chip 1-1 includes 96 groups of liquid chambers 1-1-2 and microtubes 1-1-3, and the planar size of the chip is 128×86mm 2 . The top of eac...

Embodiment 2

[0062] Simultaneous droplet sample preparation for antimicrobial susceptibility testing (AST) of 4 urinary tract infection pathogens using droplet digitization

[0063] (1) Chip function partition: such as Figure 8 As shown, the array chip set with 96 units is used for 6 kinds of antibiotic susceptibility tests of 4 kinds of urinary system infection pathogenic bacteria. Staphylococcus aureus, Enterococcus faecalis and Klebsiella pneumoniae; the antibiotic drugs tested from left to right in columns 1-6 and 7-12 are erythromycin, clindamycin, ampicillin, vancomycin, respectively , gentamicin, levofloxacin; rows D and H are corresponding control samples without any antibiotics; rows A-C and E-G correspond to antibiotic concentrations that are low, medium, and high concentrations determined with reference to the minimum inhibitory concentration MIC standard;

[0064] (2) Sample preparation: Prepare two 96-unit micro-cell array chips (referred to as micro-cell chips) and one 96-u...

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PUM

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Abstract

The invention discloses a high-throughput and rapid micro-droplet preparation device and an application thereof. The device comprises an array chip set, a temperature control module, a pressure control module, a displacement module, a vibration module and a control circuit, wherein the control circuit is connected with the vibration module, the pressure control module, the temperature control module and the displacement module; the displacement module is connected with the temperature control module and the pressure control module; the array chip set comprises a micro-tube array chip and a micro-cell array chip, an airtight cover in the pressure control module and a metal hole seat in the temperature control module are arranged at the top and the bottom of the micro-tube array chip respectively, and a movable part of a voice coil motor of the vibration module is connected with the micro-cell array module. The device has the following advantages: the structure is simple due to the absence of complicated pump valve pipes, the size is small and compact, the automation degree is high, the requirement for parallel generation of micro-droplets with different inclusions can be met, the use is flexible and the application range is wide. Therefore, the device can be used for high-throughput and rapid micro-droplet preparation.

Description

technical field [0001] The invention belongs to the field of microfluidic chip analysis, and in particular relates to a high-throughput and rapid micro-droplet preparation device and its application. Background technique [0002] Micro-droplet technology is a brand-new microfluidic technology developed in recent years to manipulate tiny volumes of liquid. Compared with the traditional continuous flow, the discrete droplets can be used as relatively independent microreactors or reservoirs, with minimal reagent consumption, which is conducive to reducing costs and achieving high throughput, and no diffusion between droplets and samples can avoid inter-sample It has the advantages of low cross-contamination, stable reaction conditions, easy manipulation, and large specific surface area that can accelerate heat / mass exchange. Therefore, it is an ideal tool for drug synthesis, single-cell analysis, molecular biology, and material synthesis. [0003] The preparation of micro-drop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/502707B01L3/502792B01L2200/027B01L2200/10B01L2200/146B01L2200/147B01L2300/0636B01L2400/0433
Inventor 舒博文刘大渔
Owner GUANGZHOU FIRST PEOPLES HOSPITAL (GUANGZHOU DIGESTIVE DISEASE CENT GUANGZHOU FIRST PEOPLES HOSPITAL GUANGZHOU MEDICAL UNIV THE SECOND AFFILIATED HOSPITAL OF SOUTH CHINA UNIV OF TECH)
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