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Method for improving growth and carbon fixation rate through improved spirulina key enzyme genes

A key enzyme gene, the technology of spirulina, applied in the field of biomass energy utilization and carbon dioxide emission reduction, can solve limited problems, increase the growth rate of carbon fixation, improve the efficiency of photosynthetic utilization and energy supply, efficient and feasible growth and solidification of spirulina Carbon rate effect

Active Publication Date: 2018-05-04
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods only increase the growth and carbon fixation rate of microalgae by changing the external conditions, but do not change the microalgae itself.
There is limited research on the genetic modification of Spirulina to increase the carbon sequestration rate of its growth

Method used

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  • Method for improving growth and carbon fixation rate through improved spirulina key enzyme genes

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Experimental program
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Effect test

Embodiment 1

[0024] (1) Using gene editing technology to adjust the gene expression levels of four key enzymes bchG, bchP, DLD, and ACO in Spirulina cells to achieve genetic improvement: the gene expression level of chlorophyll synthase bchG was increased from 120 to 310, double The gene expression of geranyl reductase bchP was increased from 80 to 630, the gene expression of dihydrolipoamide dehydrogenase DLD was increased from 100 to 320, and the gene expression of aconitic acid hydratase ACO was increased from 170 to 460 .

[0025] (2) Take the genetically improved Spirulina strain and place it in a photobioreactor for purification and expansion of a single strain in multiple test tubes. The culture conditions are as follows: Spirulina standard medium is used, the volume of algae liquid is 300 ml, the inoculation density of algae liquid is controlled at 0.1 g / L, the temperature is 27°C, the light intensity is 7000 Lux, and the continuous air flow rate is 30 ml / min.

[0026] (3) Collect...

Embodiment 2

[0028] (1) Using gene editing technology to adjust the gene expression of four key enzymes bchG, bchP, DLD, and ACO in Spirulina cells to achieve genetic improvement: the gene expression of chlorophyll synthase bchG was increased from 130 to 330, double The gene expression of geranyl reductase bchP was increased from 90 to 650, the gene expression of dihydrolipoamide dehydrogenase DLD was increased from 110 to 340, and the gene expression of aconitic acid hydratase ACO was increased from 180 to 480 .

[0029] (2) Take the genetically improved Spirulina strain and place it in a photobioreactor for purification and expansion of a single strain in multiple test tubes. The culture conditions are as follows: Spirulina standard medium is used, the volume of algae liquid is 300 ml, the inoculation density of algae liquid is controlled at 0.1 g / L, the temperature is 27°C, the light intensity is 7000 Lux, and the continuous air flow rate is 30 ml / min.

[0030](3) Collect the algae liq...

Embodiment 3

[0032] (1) Using gene editing technology to adjust the gene expression levels of four key enzymes bchG, bchP, DLD, and ACO in Spirulina cells to achieve genetic improvement: the gene expression level of chlorophyll synthase bchG was increased from 140 to 350, double The gene expression of geranyl reductase bchP was increased from 100 to 670, the gene expression of dihydrolipoamide dehydrogenase DLD was increased from 120 to 360, and the gene expression of aconitic acid hydratase ACO was increased from 190 to 500 .

[0033] (2) Take the genetically improved Spirulina strain and place it in a photobioreactor for purification and expansion of a single strain in multiple test tubes. The culture conditions are as follows: Spirulina standard medium is used, the volume of algae liquid is 300 ml, the inoculation density of algae liquid is controlled at 0.1 g / L, the temperature is 27°C, the light intensity is 7000 Lux, and the continuous air flow rate is 30 ml / min.

[0034] (3) Collec...

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Abstract

The invention relates to a biomass energy utilization technology and aims to provide a method for improving a growth and carbon fixation rate through improved spirulina key enzyme genes. The method comprises the following steps: regulating gene expression amounts of four key enzymes in spirulina cells so as to realize genetic modification by utilizing a gene editing technology; taking the spirulina plants subjected to genetic modification, and performing multi-test-tube single-plant purification and enlarged culture in a photobioreactor; and collecting spirulina liquid in each test tube everyday, testing a carbon dioxide fixation rate of the spirulina, and taking a sample with the maximum value of the carbon dioxide fixation rate as an improved spirulina plant. According to the method disclosed by the invention, a chlorophyll synthesis pathway and a tricarboxylic acid cycle pathway of the spirulina can be enhanced, the photosynthesis use efficiency and energy supply of spirulina cellsare improved, the growth and carbon fixation rate of the spirulina is further improved, and the method is a high-efficiency and feasible method for increasing the spirulina growth and carbon fixationrate.

Description

technical field [0001] The invention relates to biomass energy utilization and carbon dioxide emission reduction technology, in particular to a method for improving the key enzyme gene of spirulina to increase the growth rate of carbon fixation. Background technique [0002] With atmospheric CO 2 As the concentration increases, the problem of greenhouse effect has attracted more and more attention from all over the world. Among the many attempts to reduce atmospheric CO 2 In the method, the use of microalgae to reduce CO emissions 2 The bioengineering techniques of the bioengineering have been extensively studied for decades (Sivakumar et al. 2014). Due to their high carbon sequestration efficiency and high lipid content, microalgae have become sustainable substrates for biofuel production (Breuer et al.2012; Hu et al.2008). In addition, the absorption and utilization of high concentrations of CO in the flue gas of coal-fired power plants 2 (15vol.%) can help reduce CO ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12R1/01
CPCC12N1/20C12N9/0051C12N9/0073C12N9/1085C12N9/88C12Y108/01004C12Y205/01062C12Y402/01003
Inventor 程军岑可法周俊虎刘建忠王智化张彦威杨卫娟周志军何勇
Owner ZHEJIANG UNIV
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