2results about How to "Increase energy supply" patented technology

This invention proposes a Bacillus compound inoculant, its preparation method, and its application, relating to the field of microbial technology. The method of this invention includes the following steps: selecting 18%-25% glucose, 18%-25% yeast extract, 0.3%-0.7% manganese sulfate, 1%-3% potassium dihydrogen phosphate, 1%-3% diammonium citrate, 3%-7% sodium acetate, and 0.1%-0.5% arginine by mass fraction, with the remainder being distilled water, and thoroughly mixing them to prepare a fermentation medium; selecting Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, and Bacillus lentigines, and obtaining a seed culture for inoculation at a constant temperature; mixing the various seed cultures in a ratio of 0.1-0.5:0.1-0.5:95-98:0.1-2:0.1-2 to prepare a compound inoculant, and fermenting to obtain the Bacillus compound inoculant. The compound microbial agent prepared by this invention can not only change the soil nutrient structure and the distribution of microbial communities in the soil, but also change the content of active ingredients in the rhizome of Ligusticum chuanxiong, thereby significantly promoting the growth of Ligusticum chuanxiong rhizome.

The invention relates to the technical field of improved breeding of animal husbandry, and particularly discloses an improved NK cell culture medium and a preparation method thereof. The improved NK cell culture medium comprises a basic culture medium, a supplement and a composite sustained-release body. All the components are mixed according to a specific proportion, and the requirements of NK cell proliferation and function maintenance are met. Wherein the inner layer of the composite sustained-release body takes trehalose and human recombinant serum albumin as protective agents to wrap the interleukin, and the outer layer of the composite sustained-release body is an acrylate prepolymer photocuring polymer containing a photoinitiator, so that the continuous and stable release of the interleukin is realized. Through component optimization and slow release system design, the NK cell culture efficiency and activity are effectively improved, and the method is suitable for NK cell multiplication culture.