The method discloses a method for making biscuits with deer plasma protein polypeptide, and belongs to the field of functional food processing. The method includes the steps of firstly, conducting enzymolysis on deer plasma through neutral protease, then, conducting centrifugation, conducting ultra-filtration through an ultra-filtration membrane, and obtaining a solution with deer plasma protein polypeptide; secondly, freezing and drying the solution with deer plasma protein polypeptide to obtain deer plasma protein polypeptide powder, and evenly mixing the deer plasma protein polypeptide powder with flour, white granulated sugar, cream, shortening, grain blend oil, eggs, milk powder, composite raising agents, salt and water; thirdly, conducting rolling, forming, baking and cooling to obtain the biscuits with deer plasma protein polypeptide. The biscuits with deer plasma protein polypeptide are sweet, crispy, delicious and rich in nutrient, and can have the health care effect after being eaten frequently.