Mixed microbial agent suitable for deep-repairing petroleum hydrocarbon polluted soil in middle temperature aerobic biopile system as well as preparation and application of mixed microbial agent
A technology of mixed bacterial agents and aerobic organisms, applied in the restoration of contaminated soil, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of hindering the rapid degradation process of petroleum hydrocarbons, the deep degradation efficiency, and the growth and metabolism of unfavorable functional microorganisms , Hinder the metabolism and degradation of fatty acids and other problems, achieve the effect of maintaining microbial abundance, continuing the efficient and fast process, and enhancing mineralization
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] Preparation of mixed bacterial agent A and mixed bacterial agent B for deep degradation of petroleum hydrocarbon pollutants
[0031] The bacterial source sample used in this embodiment was collected from the petroleum-contaminated soil of Liaohe Oilfield, weighed 2.0g of the contaminated soil, added 50ml of inorganic salt medium, shook for 10min and then left it at room temperature for 5min, and drew the supernatant until it contained 1.0g L -1 Petroleum hydrocarbon or contain 0.5g·L -1 N-octadecanoic acid was enriched and cultured in inorganic salt liquid medium for 6 days. -1 Petroleum hydrocarbon or contain 0.5 g·L -1 Separation and purification on the inorganic salt solid medium of n-octadecanoic acid, select a single colony with better growth and preserve it, and inoculate it to a concentration of 30g·kg -1 Petroleum contaminated soil may contain 10g·kg -1 In n-octadecanoic acid polluted soil, with 50ml inorganic salt culture medium as liquid medium, carry out s...
Embodiment 2
[0036] Example 2 The deep degradation of petroleum hydrocarbon-contaminated soil by mixed bacterial agents in mesophilic aerobic biopile remediation technology
[0037] The mesophilic aerobic biopile system consists of a water separation and nutrient allocation system, a forced ventilation system, a piling foundation, and an anti-seepage system. The moisture and inorganic nutrient replenishment of the pile are delivered by the liquid supply pipeline distributed on the top of the pile; the ventilation in the pile is divided into forced air injection and forced air extraction, which are respectively completed by the air injection pipeline and the air extraction pipeline, and the air injection pipeline supplies 35 ~40℃ hot air to ensure the medium temperature environment of the pile; the bottom of the pile is laid with anti-seepage geotextile, and the top is covered with anti-seepage film. During the operation of the pile, take gas injection for 30 minutes, stop the ventilation f...
Embodiment 3
[0042] Example 3 The deep degradation of n-octadecane contaminated soil by mixed bacterial agents in mesophilic aerobic biopile remediation technology
[0043] The mixed bacterial agent A was added to the mesophilic aerobic biopile repair system described in Example 2. The piling system was built with n-octadecane-contaminated soil, and the number of bacteria and microorganisms in the initial piling reached 7.3 × 10 8 CFU·g -1 , when the degradation efficiency of n-octadecane reaches 29.5%, the degradation efficiency in this period 7d (28d) is 49.9% of the degradation efficiency in the initial period (in the first 7d), and the supplement of mixed bacterial agent B is carried out. The total number of bacterial microorganisms in the soil reached 91% of the initial total number of bacteria. Taking traditional microbial remediation as the control (CK), taking no supplementation of mixed bacterial agent B as treatment 1 (Biopile-1), and adding mixed microbial agent B as treatment ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com