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Mixed microbial agent suitable for deep-repairing petroleum hydrocarbon polluted soil in middle temperature aerobic biopile system as well as preparation and application of mixed microbial agent

A technology of mixed bacterial agents and aerobic organisms, applied in the restoration of contaminated soil, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of hindering the rapid degradation process of petroleum hydrocarbons, the deep degradation efficiency, and the growth and metabolism of unfavorable functional microorganisms , Hinder the metabolism and degradation of fatty acids and other problems, achieve the effect of maintaining microbial abundance, continuing the efficient and fast process, and enhancing mineralization

Pending Publication Date: 2018-05-08
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The β-oxidation process of fatty acid degradation needs to be completed in prokaryotic cells. A large amount of fatty acid accumulation causes a decrease in pH in the microenvironment, which is not conducive to the growth and metabolism of many functional microorganisms, and also hinders the further metabolism and degradation of fatty acids by general functional microorganisms. This hinders the overall rapid degradation process of petroleum hydrocarbons and their deep degradation efficiency

Method used

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  • Mixed microbial agent suitable for deep-repairing petroleum hydrocarbon polluted soil in middle temperature aerobic biopile system as well as preparation and application of mixed microbial agent
  • Mixed microbial agent suitable for deep-repairing petroleum hydrocarbon polluted soil in middle temperature aerobic biopile system as well as preparation and application of mixed microbial agent
  • Mixed microbial agent suitable for deep-repairing petroleum hydrocarbon polluted soil in middle temperature aerobic biopile system as well as preparation and application of mixed microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of mixed bacterial agent A and mixed bacterial agent B for deep degradation of petroleum hydrocarbon pollutants

[0031] The bacterial source sample used in this embodiment was collected from the petroleum-contaminated soil of Liaohe Oilfield, weighed 2.0g of the contaminated soil, added 50ml of inorganic salt medium, shook for 10min and then left it at room temperature for 5min, and drew the supernatant until it contained 1.0g L -1 Petroleum hydrocarbon or contain 0.5g·L -1 N-octadecanoic acid was enriched and cultured in inorganic salt liquid medium for 6 days. -1 Petroleum hydrocarbon or contain 0.5 g·L -1 Separation and purification on the inorganic salt solid medium of n-octadecanoic acid, select a single colony with better growth and preserve it, and inoculate it to a concentration of 30g·kg -1 Petroleum contaminated soil may contain 10g·kg -1 In n-octadecanoic acid polluted soil, with 50ml inorganic salt culture medium as liquid medium, carry out s...

Embodiment 2

[0036] Example 2 The deep degradation of petroleum hydrocarbon-contaminated soil by mixed bacterial agents in mesophilic aerobic biopile remediation technology

[0037] The mesophilic aerobic biopile system consists of a water separation and nutrient allocation system, a forced ventilation system, a piling foundation, and an anti-seepage system. The moisture and inorganic nutrient replenishment of the pile are delivered by the liquid supply pipeline distributed on the top of the pile; the ventilation in the pile is divided into forced air injection and forced air extraction, which are respectively completed by the air injection pipeline and the air extraction pipeline, and the air injection pipeline supplies 35 ~40℃ hot air to ensure the medium temperature environment of the pile; the bottom of the pile is laid with anti-seepage geotextile, and the top is covered with anti-seepage film. During the operation of the pile, take gas injection for 30 minutes, stop the ventilation f...

Embodiment 3

[0042] Example 3 The deep degradation of n-octadecane contaminated soil by mixed bacterial agents in mesophilic aerobic biopile remediation technology

[0043] The mixed bacterial agent A was added to the mesophilic aerobic biopile repair system described in Example 2. The piling system was built with n-octadecane-contaminated soil, and the number of bacteria and microorganisms in the initial piling reached 7.3 × 10 8 CFU·g -1 , when the degradation efficiency of n-octadecane reaches 29.5%, the degradation efficiency in this period 7d (28d) is 49.9% of the degradation efficiency in the initial period (in the first 7d), and the supplement of mixed bacterial agent B is carried out. The total number of bacterial microorganisms in the soil reached 91% of the initial total number of bacteria. Taking traditional microbial remediation as the control (CK), taking no supplementation of mixed bacterial agent B as treatment 1 (Biopile-1), and adding mixed microbial agent B as treatment ...

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Abstract

The invention belongs to the technical field of microbial remediation of petroleum hydrocarbon polluted soils, and particularly relates to a mixed microbial agent suitable for deep-repairing petroleumhydrocarbon polluted soil in a middle temperature aerobic organic bank system as well as preparation and application thereof. The mixed microbial agent is prepared by mixing and immobilizing bacilluslicheniformis and bacillus cereus, i.e., two functional petroleum hydrocarbon-degrading bacteria, and when degradation efficiency of petroleum hydrocarbons within a certain period of time is reducedto 50% or less of degradation efficiency in an initial period, a mixed microbial agent B prepared by mixing and immobilizing by the bacillus licheniformis and the bacillus cereus in a ratio from (1 :3) to (1 : 5) (V to V) is added to maintain total bacterial content in an organic bank up to 80% to 100% of the initial content, so as to achieve a deep degradation effect on petroleum hydrocarbon pollutants in the polluted soil, and effectively degrade toxicity of the pollutants and degradation products thereof.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of petroleum hydrocarbon-contaminated soil, and in particular relates to a mixed bacterial agent suitable for deep treatment of petroleum hydrocarbon-contaminated soil in a mesophilic aerobic bioreactor system and its preparation and application. Background technique [0002] With the increasing intensity of oil extraction, the content and probability of petroleum hydrocarbons entering the environment have also increased sharply, seriously endangering the ecological environment and even threatening people's normal life. Under such circumstances, a variety of treatment technologies expected to be applied to oil-contaminated soil remediation have been developed and researched. However, the high price of remediation technology, cumbersome implementation process, limited treatment capacity and potential secondary pollution risks all restrict the market application of soil remediation tec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/10C12R1/085
CPCB09C1/10C12N1/20C12N1/205C12R2001/085C12R2001/10
Inventor 郭书海王卅李凤梅张凯
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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