Method for in-vitro inducing antigen-specific T cells

Abstract

The invention relates to a method for in-vitro inducing antigen-specific T cells, which belongs to the technical field of biology. T cells of the human beings are induced in vitro under the combination effect of rapamycin and TGF-beta cell DC cells to become adjustable T cells having an immunity inhibition function. The method has the following advantages that (1) the quantity is sufficient; (2) the Th17 cell differentiation can be resisted; and (3) compared with the adjustable T cells induced in other methods, the inhibition capacity is higher, and the biological effect is higher. The defectsof the natural adjustable T cells can be overcome, and the method has greater treatment advantages in the aspect of treating inflammation diseases and immunity diseases and preventing immunological rejection of organ transplantation.

Description

technical field [0001] The invention relates to a method for inducing antigen-specific T cells in vitro, which belongs to the field of biomedical technology Background technique [0002] For half a century, while immunosuppressive drugs have significantly prolonged the survival time of allografts, due to their own toxic side effects, high price and clinical complications caused by drug-induced non-specific immunosuppression, a series of serious problems, Greatly limit the further development of clinical organ transplantation. It has become one of the basic topics in the field of transplantation immunology to reduce or even eliminate immunosuppressants by inducing graft-specific immune tolerance or achieving a "clinically almost tolerated" state similar to the application of immunosuppressants. Although many researchers have successfully induced immune tolerance of allografts in different animals, for reasons that are not yet clear, an effective method to expand regulatory T...

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Problems solved by technology

Such as: (1) Blocking co-stimulatory pathways: Although in transplanted animal models, the survival time of allografts can be significantly prolonged by blocking co-stimulatory pathways, but this method cannot achieve sustained and stable tolerance

(2) Immature allogeneic dendritic cells (DC): At present, immature DC induced in vitro can induce antigen-specific T cell anergy in vivo and in vitro in some cases, and in vivo infusion can significantly prolong allogeneic transplantation survival of the drug, but only short-term tolerance

[0005] In recent years, Treg is mainly expanded by magnetic beads coated with CD3 / CD28. Although this expansion method can obtain a large number of cells, this technology also has its disadvantages: 1) the culture can easily lead to the decrease of Foxp3 in cells, 2) The longer the expansion time, the lower the function of Treg, 3) The cost of culture is expensive

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