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Novel method for extracting eleutheroside from radix acanthopanacis senticosi

A technique for acanthopanax senticosus and acanthopanax senticosus, which is applied to the purification of acanthopanax senticosus, and the field of acanthopanax senticosus extraction from acanthopanax senticosus, can solve the problems of low extraction purity and low extraction rate, and achieve high extraction purity. , the adsorption is fast, the separation effect is obvious

Inactive Publication Date: 2018-05-11
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a new method for extracting eleutherosides from Acanthopanax

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: a kind of new method of extracting eleutheroside from Acanthopanax, its steps are as follows:

[0021] (1) Crush Acanthopanax to 40 meshes, add fermentation broth, extract with 70% methanol, solid-liquid ratio 1:30g / mL, ultrasonic treatment at 45°C for 30min and microwave power 400W, microwave time 3min and double-assisted. The extract is concentrated under reduced pressure to obtain the initial extract of Acanthopanax;

[0022] (2) macroporous resin is adsorbed on the gained solution, and the model is LX-100 resin;

[0023] (3) Under normal temperature and pressure, with a flow rate of 1BV / h, first elute with distilled water, with an elution volume of 3-5BV, to wash away water-soluble impurities such as pigments, tannins, and inorganic salts in the Acanthopanax aqueous solution;

[0024] (4) Use 70%, 75%, 80%, 85%, 90%, 95% ethanol solution to elute at a flow rate of 1-3BV / h and an elution volume of 6-8BV. Layer tomography tracking and monitoring;

[0...

Embodiment example 2

[0027] Example 2: A new method for extracting eleutheroside from Acanthopanax, the steps are as follows:

[0028] (1) Crush Acanthopanax to 60 mesh, add fermentation broth, extract with 70% methanol, solid-liquid ratio 1:20g / mL, ultrasonic treatment at 45°C for 30min and microwave power 400W, microwave time 5min double-assisted. The extract is concentrated under reduced pressure to obtain the initial extract of Acanthopanax;

[0029] (2) macroporous resin is adsorbed on the gained solution, and the model is LX-100 resin;

[0030] (3) Under normal temperature and pressure, with a flow rate of 1BV / h, first elute with distilled water, with an elution volume of 3-5BV, to wash away water-soluble impurities such as pigments, tannins, and inorganic salts in the Acanthopanax aqueous solution;

[0031] (4) Use 70%, 75%, 80%, 85%, 90%, 95% ethanol solution to elute at a flow rate of 1-3BV / h and an elution volume of 6-8BV. Layer tomography tracking and monitoring;

[0032] (5) Collect...

Embodiment example 3

[0034] Example 3: A new method for extracting eleutheroside from Acanthopanax, the steps are as follows:

[0035] (1) Crush Acanthopanax to 80 meshes, add fermentation broth, extract with 70% methanol, solid-liquid ratio 1:25g / mL, ultrasonic treatment at 45°C for 30min and microwave power 400W, microwave time 5min double-assisted. The extract is concentrated under reduced pressure to obtain the initial extract of Acanthopanax;

[0036] (2) macroporous resin is adsorbed on the gained solution, and the model is AB-8 resin;

[0037] (3) Under normal temperature and pressure, with a flow rate of 1BV / h, first elute with distilled water, with an elution volume of 3-5BV, to wash away water-soluble impurities such as pigments, tannins, and inorganic salts in the Acanthopanax aqueous solution;

[0038] (4) Use 70%, 75%, 80%, 85%, 90%, 95% ethanol solution to elute at a flow rate of 1-3BV / h and an elution volume of 6-8BV. Layer tomography tracking and monitoring;

[0039] (5) Collect...

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Abstract

Ultrasonic and microwave dual-assistance is applied to purification of eleutheroside, so that the adsorption selectivity for eleutheroside is good, adsorption and desorption are fast, and the adsorption capacity is larger. Extraction is convenient and fast, the production cost is low, the separation effect is obvious, the extraction purity is high, 90% or higher of eleutheroside can be obtained, and the defects of relatively low conventional extraction rate and low extraction purity are overcome. Polyamide resin adsorption chromatography with stable physical and chemical properties, larger surface area, higher exchange speed, high mechanical strength, high anti-pollution capacity and good thermal stability is adopted, selectivity to organic matter is better, influence of existence of inorganic salts and strong ionic low molecular compounds is avoided, and morin can be adsorbed selectively from the solution by physical adsorption. The method has the advantages of fast adsorption, fast desorption and larger adsorption capacity.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for purifying eleutheroside, in particular to a new method for extracting eleutheroside from eleuthero. Background technique: [0002] Acanthopanax, shrub, 1-6 meters high; many branches, one or two years old usually with dense thorns, rarely only thorns on the nodes or no thorns; thorns are straight and slender, needle-like, downward, the base is not enlarged, Circular puncture marks left after falling off, leaflets 5, rare 3; petioles often sparsely thorny, 3-10 cm long; leaflets papery, elliptic obovate or oblong, 5-13 cm long, 3 cm wide -7 cm, apex acuminate, base broadly cuneate, upper rough, dark green, coarse hairs on veins, lower light green, pubescent on veins, sharply serrated at edges, 6-7 pairs of lateral veins, distinct on both sides, The network veins are not obvious; the petioles are 0.5-2.5 cm long, brown pubescent, and sometimes have fine spines....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/254
CPCA61K36/254A61K2236/19A61K2236/333A61K2236/55
Inventor 姚德坤邹静李玉伟万莉王雷
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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