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Preparation method of gene-carrying multifunctional contrast agent and application of gene-carrying multifunctional contrast agent

A multi-functional, contrast agent technology, applied in the field of ultrasound imaging, can solve the problems of ultrasonic-triggered microsphere phase transition difficulties, adverse biological effects, etc., and achieve the effect of regular morphology and good stability

Active Publication Date: 2018-05-11
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of Laplace pressure and surface stabilizers makes it relatively difficult for ultrasound to trigger the phase transition of microspheres. Therefore, the pressure and frequency of traditional diagnostic ultrasound are not enough to stimulate the phase transition of microspheres, and the ultrasonic pressure and frequency may also cause adverse biological effects

Method used

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  • Preparation method of gene-carrying multifunctional contrast agent and application of gene-carrying multifunctional contrast agent
  • Preparation method of gene-carrying multifunctional contrast agent and application of gene-carrying multifunctional contrast agent
  • Preparation method of gene-carrying multifunctional contrast agent and application of gene-carrying multifunctional contrast agent

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Embodiment Construction

[0039] 1. A gene-loaded multifunctional contrast agent (FA-CN-PFP-ICG / pDNA, referred to as FCNPI / pDNA) of the present invention, the specific preparation method of which is:

[0040] 1), dissolving:

[0041] DPPC (dipalmitoylphosphatidylcholine, American Avanti Company), DSPE-(PEG2000)-Folate (folate-modified polyethylene glycol-(2000)-distearoylphosphatidylethanolamine, American Avanti Company), Dc -cholesterol (Dc-cholesterol, American Avanti Company) weighed a total of 10 mg according to the mass ratio of 5:2:2 and added it to the round bottom flask. At the same time, measured 6 ml of chloroform and added it to the round bottom flask. °C in water and heated until fully dissolved.

[0042] 2) Rotary evaporation: After dissolving for 20 minutes, fix the round-bottomed flask on a rotary evaporator (temperature: 50°C) and perform a rotary evaporation under reduced pressure to remove the organic solvent. Rotational speed: 80rpm, time: 2 hours, forming a layer of uniform white ...

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Abstract

The invention discloses a gene-carrying multifunctional contrast agent. The gene-carrying multifunctional contrast agent comprises a lipid shell membrane; the lipid shell membrane is modified with folic acid, and the surface of the lipid shell membrane is provided with positive charges and carries a gene. The invention aims to provide a preparation method for manufacturing a gene-carrying multifunctional contrast agent as well as application of the gene-carrying multifunctional contrast agent, wherein the multifunctional contrast agent has in-vivo and in-vitro targeting capability, has abilityof enhancing ultrasonic / photoacoustic developing, and has the gene transfection advantage under the action of laser in vivo and in vitro.

Description

technical field [0001] The invention relates to the field of ultrasonic imaging, in particular to a preparation method and application of a gene-loaded multifunctional contrast agent. Background technique [0002] Retinoblastoma (RB) is the most common intraocular malignant tumor in infants and young children. It mostly occurs within 3 years old. It is highly malignant and difficult to diagnose early. At present in China, most children with "white pupil syndrome" have already grown RB when they go to the doctor, which indicates that they have entered a period that seriously endangers vision and even life, and may not be able to save the eyeball. The main treatment is enucleation of the affected eye. Supplemented by radiotherapy, chemotherapy and other means. The eyeball enucleation surgery makes the child lose the function of one eye, and will never have stereo vision, and seriously affects the appearance and physical and mental health of the child; while radiotherapy and c...

Claims

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Application Information

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IPC IPC(8): A61K49/22A61K9/51A61K31/711A61P35/00
CPCA61K9/5146A61K31/711A61K49/0002A61K49/223A61K49/225
Inventor 周希瑗武明星邹宏密
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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