A kind of preparation method of plant-derived chitosan

A plant-derived chitosan technology, applied in the field of preparation of plant-derived chitosan, can solve problems such as environmental pollution, stench, and time-consuming, and achieve the effects of protecting the environment, reducing usage, and reducing production costs

Active Publication Date: 2020-03-31
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the large-scale production of chitosan is to use the by-products of seafood as raw materials to complete the deacetylation of chitin under high-concentration strong alkali, strong acid and high temperature to realize chitosan. Disadvantages such as waste liquid, stench and environmental pollution

Method used

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  • A kind of preparation method of plant-derived chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Take 1g of dried Ganoderma lucidum spore powder, add deionized water to the Ganoderma lucidum spore powder at a material-to-liquid ratio of 1:15 (g:ml), and extract the Ganoderma lucidum polysaccharide by ultrasonic (100W) in a water bath at 45°C for 1 time and then use 7000rpm Centrifuge for 10 minutes to remove the supernatant to obtain a sediment; add 95v / v% ethanol to the sediment at a material-to-liquid ratio of 1:15 (g:ml), and ultrasonically treat it at room temperature (25℃±1℃) for 1h to remove three Terpenoids, and then centrifuged under the same conditions to obtain the secondary sediment; add deionized water to the secondary sediment according to the material-to-liquid ratio of 1:30 (g:ml), soak for 3 hours at 25°C to remove water-soluble proteins. After centrifugation, the three precipitates obtained are dried at 55°C to obtain crude chitin;

[0029] (2) Take 1g of crude chitin and add 30% H 2 O 2 10ml, keep the temperature in a water bath at 70℃ for 1h. ...

Embodiment 2

[0045] (1) Take 1g of dried Ganoderma lucidum spore powder, add deionized water to the Ganoderma lucidum spore powder at a material-to-liquid ratio of 1:25 (g:ml), and extract 3 times of Ganoderma lucidum polysaccharides at 55℃ with ultrasonic (150W) at 10000rpm Centrifuge for 5 minutes to remove the supernatant to obtain a sediment; add 95v / v% ethanol to the sediment at a material-to-liquid ratio of 1:25 (g:ml), and ultrasonicate for 2h at room temperature (25℃±1℃) to remove the three Terpenoids, then centrifuged under the same conditions to obtain the secondary sediment; add deionized water to the secondary sediment according to the material-to-liquid ratio of 1:40 (g:ml), soak for 2h at 35°C to remove water-soluble proteins, After centrifugation, the three precipitates obtained are dried at 60°C to obtain crude chitin;

[0046] (2) Take 1g of crude chitin and add 30% H 2 O 2 20ml, keep the temperature in a water bath at 60℃ for 2h. Add 1wt% NaOH solution to adjust the pH to ...

Embodiment 3

[0050] (1) Take 1g of dried Ganoderma lucidum spore powder, add deionized water to the Ganoderma lucidum spore powder at a material-to-liquid ratio of 1:25 (g:ml), and extract 5 times of Ganoderma lucidum polysaccharides at 65℃ by ultrasonic (200W) at 8000rpm Centrifuge for 10 minutes to remove the supernatant to obtain a precipitate; add 95v / v% ethanol to the precipitate according to the material-liquid ratio of 1:25 (g:ml), and ultrasonicate for 3 hours at room temperature (25°C±1°C) to remove the three Terpenoids, then centrifuged under the same conditions to obtain the secondary sediment; add deionized water to the secondary sediment according to the material-to-liquid ratio of 1:50 (g:ml), soak at 45℃ for 1 hour to remove water-soluble proteins, After centrifugation, the three precipitates obtained are dried at 60°C to obtain crude chitin;

[0051] (2) Take 1g of crude chitin and add 30% H 2 O 2 30ml, keep the temperature in a water bath at 50℃ for 3h. Add 1wt% NaOH soluti...

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Abstract

The invention discloses a preparation method of plant-derived chitosan. The method comprises the steps that 1, deionized water is added to ganoderma lucidum spore powder, water-bath ultrasonic extraction and centrifugation are conducted, ethyl alcohol is added to the mixed solution, the mixed solution is subjected to ultrasonic treatment and centrifuged, deionized water is added to the mixed solution, and soaking, centrifugation and drying are conducted to obtain rough chitin; 2, the rough chitin is placed in an H2O2 solution, the mixed solution is placed in water bath, adjusted to be neutralby using a NaOH solution and centrifuged, an alkaline solution is added to the mixed solution, water bath treatment is conducted, the mixed solution is adjusted to be neutral by using an HCI solutionand dried, and a chitosan rough product is obtained. According to the preparation method of plant-derived chitosan, the ganoderma lucidum spore powder is used as a raw material and quickly and conveniently converted into chitosan through a low-concentration chemical solvent and a low-temperature reaction condition, and the preparation method is suitable for large-scale production. The chitosan prepared by means of the method is stable in property, the integrated utilization value of resources is increased, the production cost of chitosan is lowered, the environmental pollution is reduced, chitosan has excellent biological characteristics, novel plant-derived chitosan is effectively provided, and the source of the raw material of chitosan is widened.

Description

Technical field [0001] The invention relates to the field of chitosan, in particular to a preparation method of plant-derived chitosan. Background technique [0002] Chitin is a precursor of chitosan, a biopolymer that is abundant on earth, second only to cellulose, and is widely found in marine crustaceans, plankton, insect exoskeletons, seaweed and fungi. According to an article published in Volume 524 of Nature in 2015, the global annual biosynthesis of chitin reaches 100 billion tons. However, because chitin is difficult to dissolve in general solutions, complicated preparation steps are often required, which hinders the feasibility of wide application. On the other hand, due to the decay and deterioration characteristics of seafood, a large number of chemical solvents and additives are used in the preparation of chitin, and at the same time, it also limits the cost and cumbersome process of preparation. Chitosan is a derivative of chitin, which can be obtained by deacetyla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/003
Inventor 张明暐朱礼芳陈仙乐李劲松吴子敬汪光昆
Owner ZHEJIANG UNIV
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