Method for control and in-situ detection of enzymatic activity under irradiation of electronic beams

Abstract

The invention discloses a method for control and in-situ detection of enzymatic activity under irradiation of electronic beams. The method includes the following steps of 1, preparing a titanium dioxide thin-film field-effect tube; 2, carrying out enzyme immobilization; 3, putting a device into an SEM sample table, and adopting silicon as a gate electrode, wherein any two adjacent electrodes in agold electrode array are adopted as a source electrode and a leakage electrode, and the electrodes are connected with aerial connectors on a cavity through leads; 4, putting the device of the electrodes into a scanning electron microscope, and using the electronic beams for radiation; 4, conducting a conductance testing. On the basis of the titanium dioxide thin-film field-effect tube, enzymes arefixed to the field-effect tube, the electronic beams of the scanning electron microscope are adopted as the irradiation source, the electrodes of the field-effect tube are drawn to a semiconductor property analysis instrument outside an SEM cavity through the leads and a cavity connection port for conducting in-situ conductance measurement, and the enzyme activity is monitored through changes ofconductance. The generation of the electronic beams does not depend on any radioactive element, and in-situ monitoring of the enzyme activity under the irradiation of the electronic beams is achieved.

Description

technical field [0001] The invention relates to a technique for controlling and in-situ detection of enzyme activity by electron beam irradiation. Background technique [0002] Irradiation technology is to ionize substances through irradiation sources (x-rays, γ-rays or electron beams), and to provide disinfection and change the properties of substances through the physical effects, chemical effects and biological effects of the interaction between ionizing radiation and substances. and other related technologies. Electron beam irradiation is a cold sterilization technology. Compared with traditional x-rays and γ-rays, electron beams are generated with electric energy and do not require any radioactive elements. Therefore, electron beam irradiation technology has greater advantages and safety sex. In recent years, with the continuous deepening of research on radiation technology in the fields of chemistry and food, the research on the effect of radiation on proteins and pe...

AI Technical Summary

Problems solved by technology

Then, as an important radiation source, whether the electron beam will change the structure of the enzyme after irradiating the enzyme and then affect its activity, and whether it can realize in-situ monitoring of the enzyme activity has not been reported yet.

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