Carboxymethyl chitosan nano microsphere loaded with photodynamic antibiotic and synthesis method of nano microsphere

A technology of carboxymethyl chitosan and nano-microspheres, applied in photodynamic therapy, antibacterial drugs, pharmaceutical formulations, etc., can solve the problem of being unable to prevent drug-resistant bacteria, and achieve drug resistance, obvious effects, and simple The effect of synthetic methods

Active Publication Date: 2018-05-15
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Including the number of layers through self-assembly technology, the synthesis of intelligent multi-layer film, so that it has temperature responsiveness or responsiveness, micro-

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Add 18.15ml of cyclohexane, 4.75g of Triton-100, and 4.41ml of n-octanol into a 50ml centrifuge tube, homogenize with a homogenizer for 7min, add 1.1ml of 3% carboxymethyl chitosan solution, shake evenly, Add 200 μl of 10 mg / ml toluidine blue solution, react overnight, wash once with isopropanol, and wash twice with alcohol.

[0021] The formation of microspheres was observed by transmission scanning electron microscope, and the size was uniform. Fast release.

Embodiment 2

[0023] Add 18.15ml of cyclohexane, 4.75g of Triton-100, 4.41ml of n-octanol into a 50ml centrifuge tube, homogenize with a homogenizer for 7min, add 1.1ml of 3% carboxymethyl chitosan solution, shake evenly , add 10 mg / ml toluidine blue solution and 200 μl of 1 mmol / L genipin solution, react overnight, wash once with isopropanol, and wash twice with alcohol. The microspheres synthesized above were used to test the cytocompatibility of the microspheres with mouse fibroblasts. Use microspheres and light treatment to observe the bactericidal effect (Staphylococcus aureus, E. coccus) and scanning electron microscopy to observe the characteristics of anti-biofilm (methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa).

[0024] The product has no blue substance, and the microspheres with uniform size are synthesized under the transmission electron microscope, and the particle size measured by the nanometer particle size analyzer is about 200nm. The results showed th...

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PUM

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Abstract

The invention relates to a carboxymethyl chitosan nano microsphere loaded with a photodynamic antibiotic and a synthesis method of the nano microsphere. The PH responsive nano microsphere is synthesized; the nano microsphere is hydrolyzed under an acidic condition, and immediately releases much photosensitizer to achieve a rapid and effective antibiotic effect; under a neutral or alkaline condition, the nano microsphere performs slow release, and can perform long-acting antibiosis for a week; the synthesis method is simple; a main raw material of carboxymethyl chitosan is an excellent biocompatible material; a cross-linking agent utilized is low in toxicity; a synthesis process of the nano microsphere is simple and convenient; organic reagents used are fewer; main materials are good in biocompatibility; the nano microsphere has small influence on a normal structure after the nano microsphere enters a human body; and the nano microsphere has an obvious destruction and killing effects ona bacterial biofilm and an obvious effect on drug-resistance bacteria, and can be effectively applied to in-vivo antibiosis and anti-biofilm treatment.

Description

technical field [0001] The invention specifically relates to the technical field of new materials, in particular to a carboxymethyl chitosan nanometer microsphere loaded with photodynamic bactericidal drugs and a synthesis method thereof. Background technique [0002] Bacterial infection is an important cause of human diseases, such as respiratory system diseases and reproductive system diseases are mostly caused by bacterial infection. Bacterial infections can also cause strokes and worsen other medical conditions. Various clinical traumatic operations in the hospital, such as surgical biopsy, built-in catheters (such as urinary catheters, intravenous catheters) will destroy the integrity of the skin, which can lead to the invasion of pathogens, as we know, the most important and effective treatment for bacterial infections is the use of antibiotics . In addition bacteria can form biofilms in different implants, most commonly orthopedic implants and indwelling catheters. ...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K41/00A61K47/36A61P31/04
CPCA61K9/5161A61K41/0057
Inventor 王佰亮孙林金滢滢李溪陈浩南开辉
Owner WENZHOU MEDICAL UNIV
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