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Method for cultivating aluminium toxicity-resistant pea seedlings

A technology of poison peas and seedlings, applied in botany equipment and methods, planting substrates, cultivation, etc., can solve the problem that the soil at the bottom of the core is difficult to play a role, and achieve the effect of excellent anti-aluminum poisoning ability

Inactive Publication Date: 2018-05-18
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, measures to reduce or eliminate aluminum toxicity mainly included applying lime, applying calcium-magnesium-phosphate fertilizers, and applying organic matter to improve the soil to reduce the activity of aluminum in the soil solution and provide a good environment for plant growth. However, these measures can only solve the problem of aluminum in the topsoil. Toxicity problem, it is difficult to play a role in the soil at the bottom of the heart

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for cultivating aluminum-resistant poisonous pea seedlings, comprising the following steps:

[0017] 1) Aeroponics: Pea seeds were soaked in 7.5% sodium hypochlorite for 20 minutes, washed 5 times with sterile ultrapure water, and then treated with 0.5 mmol / L CaCl in the dark at 24°C. 2 After soaking in the solution for 8 hours, spray culture to obtain pea seedlings;

[0018] 2) Hydroponics: When the pea seedlings are spray-cultured until the root length is 3 cm, the peas are transferred to a tank containing 25 μmol / L of H 3 BO 3的 Hoagland's nutrient solution was cultivated in an opaque plastic beaker, and a polydiallyldimethylammonium chloride solution with a concentration of 0.005 mg / mL at a pH of 7.0 was added, fully adsorbed for 2 hours, and then a concentration of a pH of 5.5 was added. 50mM / L silicon solution, fully adsorbed for 2h, and then use CaCl with a concentration of 0.5mmol / L at pH=4.5 2 The solution was subjected to adaptive treatment, and the...

Embodiment 2

[0020] A method for cultivating aluminum-resistant poisonous pea seedlings, comprising the following steps:

[0021] 1) Aeroponics: Pea seeds were soaked in 7.5% sodium hypochlorite for 20 minutes, washed 6 times with sterile ultrapure water, and then treated with 0.5 mmol / L CaCl in the dark at 24°C. 2 After soaking in the solution for 8 hours, spray culture to obtain pea seedlings;

[0022] 2) Hydroponics: When the pea seedlings were spray-cultured until the root length was 2 cm, the peas were transferred to a tank containing 25 μmol / L of H 3 BO 3的 Hoagland's nutrient solution was cultivated in an opaque plastic beaker, and a polydiallyldimethylammonium chloride solution with a concentration of 0.005 mg / mL at a pH of 7.0 was added, fully adsorbed for 2.5 hours, and then added with a pH of 5.5 Silicon solution with a concentration of 50mM / L, fully adsorb for 2.5h, and then use CaCl with a concentration of 0.5mmol / L at pH=4.5 2 The solution was subjected to adaptive treatmen...

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Abstract

The invention discloses a method for cultivating aluminium toxicity-resistant pea seedlings. The method comprises the following processing steps of: (1) aeroponic culture: soaking pea seeds in a 7.5%sodium hypochlorite solution, performing washing with sterile ultrapure water for 5-6 times, then soaking the washed pea seeds in a CaCl2 solution with a concentration of 0.5 mmol / L under the conditions of 24 DEG C and darkness for 8 hours, and then performing aeroponic culture to obtain the pea seedlings; and (2) water culture: when roots of the pea seedlings grow to a length of 2-3 cm during theaeroponic culture, transferring the pea seedlings to an opaque plastic beaker which is filled with a Hogland's nutrient solution for cultivation, adding a poly diallyldimethyl ammonium chloride solution with a concentration of 0.005 mg / mL and a pH value of 7.0 for full absorption, adding a silicon solution with a concentration of 50 mM / L and a pH value of 5.5 for full adsorption, and performing adaptive treatment by using a CaCl2 solution with a concentration of 0.5 mmol / L and a pH value of 4.5, wherein the period of the water culture is 6 days. According to the method, a layer of nano silicais deposited on surfaces of border cells of the roots of the pea seedlings by modifying the method for cultivating the pea seedlings and introducing a chemical modification method, so that the pea seedlings are provided with excellent aluminum toxicity resistance.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a cultivation method. Background technique [0002] According to the different parts of detoxification, the physiological mechanism of plant resistance to aluminum toxicity can be divided into two categories: external rejection and internal tolerance. The former detoxification site is in the apoplast, while the latter is the symplast. The external exclusion mechanism includes the fixation of the cell wall to aluminum, the selective permeability of the plasma membrane to aluminum, the induced barrier, the secretion of chelating ligands and phosphate, and the "active transport out of the cell". The internal tolerance mechanism mainly includes The chelation of aluminum by organic acids, proteins and other organic ligands in the cytoplasm, the compartmentalization of vacuoles, the induction of anti-aluminase and the improvement of enzyme activity, etc. In the past, measures to reduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G24/30
CPCA01G31/00
Inventor 喻敏郭少雪李学文萧洪东刘家友冯英明
Owner FOSHAN UNIVERSITY