Noninvasive pulmonary fibrosis model building method

A technology for lung fibrosis and method establishment, applied in the field of animal models, to achieve the effect of increased airway collagen deposition thickness, less trauma, and easy operation

Inactive Publication Date: 2018-05-18
SHANGHAI CHEST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] And about PM 2.5 Patents, scientific papers and other medical literature, mainly about how to prevent and treat PM 2.5 Reports of causing disease, about the use of PM in the present invention 2.5 The method of establishing a mouse model of pulmonary fibrosis by intranasal instillation has not been reported yet

Method used

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  • Noninvasive pulmonary fibrosis model building method
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  • Noninvasive pulmonary fibrosis model building method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Materials and Methods

[0028] 1. Materials

[0029] 8-10 weeks old SPF grade C57 / BL6 mice, weighing 22-25g, were purchased from Shanghai Xibi Pukai Company and raised in the barrier system of the animal center of our hospital. This study was approved by the hospital ethics committee. The gas anesthesia machine for small animals was provided by Shanghai Yuyan Instrument Company. Isoflurane was provided by Shanghai Yuyan Instrument Company. Pulmonary function instrument (Forced Maneuvers system) was provided by EMMS Company in the United Kingdom. Cytocentrifuge (Shandon Cytospin) was provided by American Thermo Fisher Company. Liu's staining solution was provided by Zhuhai Beisuo Biotechnology Co., Ltd. The hydroxyproline kit was purchased from Nanjing Jiancheng Institute of Bioengineering.

[0030] 2. Atmospheric PM2.5 sampling and processing

[0031] From October 2016 to April 2017, Laoying 2030 type medium flow PM was used in non-industrial areas of Shanghai 2....

Embodiment 2

[0065] PM 2.5 The preparation method of the suspension is the same as in Example 1, and the nasal cavity of the rat is dripped with PM 2.5 Suspension to prepare rat pulmonary fibrosis model.

Embodiment 3

[0067] PM 2.5 The preparation method of the suspension is the same as in Example 1, and the guinea pig nasal cavity is dripped with PM 2.5 Suspension to prepare guinea pig pulmonary fibrosis model.

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Abstract

The invention relates to a pulmonary fibrosis animal model building method. The method comprises the step that a pulmonary fibrosis animal model is prepared by dripping a PM2.5 suspension into a nasalcavity. The method has the advantages that a mouse pulmonary fibrosis model is built through PM2.5 nasal cavity dripping for the first time, the feasibility is verified through experiments, and by means of PM2.5 nasal cavity dripping for eight consecutive weeks, mouse pulmonary fibrosis can be induced. According to the pulmonary fibrosis animal model building method, bleomycin is not used, an airpipe is not used for dripping, operation is easy and convenient, and the trauma is small.

Description

technical field [0001] The invention relates to the technical field of animal models, in particular to a method for establishing a non-invasive pulmonary fibrosis model. Background technique [0002] With the process of industrialization and urbanization, air pollution is increasing, fine particulate matter (PM 2.5 ) as the most important air pollutant, which seriously threatens human health. Epidemiological surveys show that PM in the air 2.5 The increase in concentration is closely related to the onset and aggravation of symptoms of cardiopulmonary diseases such as coronary heart disease and chronic obstructive pulmonary disease (COPD). PM 2.5 The aerodynamic particle size is small, and after breathing into the lungs, it is easy to deposit in the distal small airways and alveoli, and enter the lung tissue space from the airway epithelium, and even enter the blood circulation, which can induce inflammation and oxidative stress damage , causing increased vascular permeab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00G01B11/02G01B11/06G01B11/28G01N1/30G01N1/36G01N21/31
CPCA61D7/00G01B11/02G01B11/06G01B11/28G01N1/30G01N1/36G01N21/31
Inventor 李锋徐蒙蒙张妍蓓张海陈宇清周新
Owner SHANGHAI CHEST HOSPITAL
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