Weissella cibaria as well as application and screening inspection method thereof

A technology of Weissella sinensis and colony, applied in the field of microorganisms, can solve the problems of not meeting the market demand, single variety of lactic acid bacteria, etc., and achieve the effects of improving palatability, reducing fiber content and improving fermentation quality

Active Publication Date: 2018-05-22
SICHUAN AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lactic acid bacteria is a silage microbial additive that is being promoted in recent years. Its main function is to adjust the composition of microorganisms in silage materials, quickly become the dominant flora, compete to inhibit the growth of harmful microorganisms and convert limited sugar, reduce the pH value of silage, and improve the quality of silage. Different silage materials have different fermentation characteristics, and the current lactic acid bacteria suitable for silage are single, which cannot meet market demand

Method used

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  • Weissella cibaria as well as application and screening inspection method thereof
  • Weissella cibaria as well as application and screening inspection method thereof
  • Weissella cibaria as well as application and screening inspection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0048] Physiological and biochemical assays: analyze the physiological and biochemical characteristics of Weissella esophagus in Example 1, and the results are shown in Table 2.

[0049] Different temperature test: the Weissella esophagus in Example 1 was inoculated into the MRS liquid medium with an inoculum size of 3%, cultured under the temperature conditions in Table 2, and its growth status was observed. The results are shown in Table 2.

[0050] Salt tolerance test: with the Weissella esophagus in embodiment 1 in the MRS liquid culture medium that contains NaCl concentration 3.0% (w / v) and 6.5% (w / v), after cultivating at 30 ℃ for 2 days, measure Its salt tolerance, the results are shown in Table 2.

[0051] Acid resistance test: After culturing the Weissella anthurium in Example 1 in the MRS liquid medium with the pH value in Table 2 at 30° C. for 7 days, the acid resistance was measured, and the results are shown in Table 2.

[0052] Sugar fermentation test: The chara...

Embodiment 3

[0065] Using Weissella phalloides of the present invention as pasture silage additive

[0066] Forage grass with a height of 1.5-2m was collected from the Chongzhou base of Sichuan Agricultural University, cut into a length of about 20mm, and fresh samples were obtained. For each treatment, 300g of fresh samples were taken and put into plastic bags (25×35cm; Aodeju, China). Weissella esophagus of the present invention in example 1 1.0 * 10 8 Add 1ml of CFU / g fresh material to each bag of silage, then vacuum seal it, place it at room temperature to ferment, each treatment group has 3 repetitions, and open the bag after 60 days of silage to obtain silage samples.

[0067] 1. Sensory evaluation:

[0068] 1) Silage samples According to the sensory silage scoring standard and grade evaluation compiled by the German Farming Association (DLG) in 1899, sensory scores were performed on the smell, structure and color of the silage.

[0069] Table 4 Sensory Evaluation Criteria for Sila...

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Abstract

The invention discloses weissella cibaria as well as application and a screening inspection method thereof. The weissella cibaria is preserved in CGMCC (China General Microbiological Culture Collection Center), and has the preservation number of CGMCC No.15075. The weissella cibaria has the advantages that the growth speed is high; the acid producing efficiency is high; the weissella cibaria can improve the quality of forage grass silage through being used as forage grass silage additives, and has wide application prospects.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to Weissella esophagus and its application and screening method. Background technique [0002] Silage is a storage method that converts the fermentation substrate in raw materials into acidic substances such as lactic acid through the proliferation of lactic acid bacteria, and maintains an acidic anaerobic environment to facilitate the long-term preservation of silage crops. Silage is yellow-green in color, sour in smell, soft and juicy, and has good palatability. It is widely used in animal husbandry production, especially in ruminant breeding, and has become an important source of high-quality roughage. [0003] Lactic acid bacteria is a silage microbial additive that is being promoted in recent years. Its main function is to adjust the composition of microorganisms in silage materials, quickly become the dominant flora, compete to inhibit the growth of harmful microorganisms and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/689C12Q1/04A23K30/18C12R1/01
CPCA23K30/18C12N1/02C12N1/20C12Q1/689C12R2001/01C12N1/205A23V2400/169
Inventor 关皓闫艳红张新全帅杨李小铃李小梅黄琳凯聂刚汪霞黄婷
Owner SICHUAN AGRI UNIV
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