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Polypeptide self-assembly nanometer carrier and preparation method thereof

A nano-carrier and self-assembly technology, which is applied in the preparation method of peptides, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of lack of tumor cells, toxic side effects of tumor cells, NSCLC difficult to cure, etc., to overcome the selection sexual effect

Inactive Publication Date: 2018-05-29
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of effective selectivity of anticancer drugs used in traditional chemotherapy for tumor cells, the toxic side effects on normal tissues and the drug resistance of tumor cells limit the anticancer efficacy, making it difficult to cure NSCLC
At present, there is no related report that the gene fusion peptide KALA and octadecanoic acid (SA) are sequentially connected to the nuclear localization signal NLS polypeptide to prepare the polypeptide self-assembled nanocarrier NLS-KALA-SA

Method used

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  • Polypeptide self-assembly nanometer carrier and preparation method thereof
  • Polypeptide self-assembly nanometer carrier and preparation method thereof
  • Polypeptide self-assembly nanometer carrier and preparation method thereof

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Embodiment 1

[0036] The chemical structure schematic diagram of the polypeptide self-assembled nanocarrier NLS-KALA-SA of the present invention is as follows: figure 1 As shown, the preparation method includes the following steps (the amino acid sequence of the gene fusion peptide KALA involved in the preparation method is shown in SEQ ID NO: 1, and the amino acid sequence of the NLS polypeptide is shown in SEQ ID NO: 2):

[0037] 1) Take 1g of 2-chlorotrityl chloride resin and place it in the reaction tube, add 1ml of N,N dimethylformamide to soak the resin for 30 minutes to fully swell the resin, and then drain the N,N dimethylformamide. Wash the material twice with dichloroethane;

[0038] 2) Take 33.7 mg (0.1 mmol) of the amino acid Fmoc-L-Pro-OH (the last amino acid in the NLS polypeptide amino acid sequence) and dissolve it with 1 ml of N, N dimethylformamide, and transfer the resulting solution into the above reaction tube. Add 0.5ml of N,N-diisopropylethylamine and react for 60min at ro...

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Abstract

The invention discloses a polypeptide self-assembly nanometer carrier and a preparation method thereof. The method comprises the following steps: sequentially connecting fusogenic peptide KALA and stearic aci to LNS polypeptide through an Fmoc solid-phase synthesis method, so as to obtain a polypeptide self-assembly nanometer carrier NLS-KALA-SA, wherein the amino acid sequence of the fusogenic peptide KALA is as shown in SEQ ID NO:1; and the amino acid sequence of the LNS polypeptide is as shown in SEQ ID NO:2. The polypeptide self-assembly nanometer carrier has the capacities of actively penetrating a cell membrane and transmembrane transport and nucleus entering at the same time, and leading an SA alkyl chain into a polypeptide molecule can stabilize the secondary structure of alpha-helical peptide and induce polypeptide to form a more stable self-assembly body.

Description

Technical field [0001] The invention relates to a polypeptide self-assembled nano carrier and a preparation method thereof, and belongs to the technical field of self-assembled nano materials. Background technique [0002] Lung cancer is one of the common malignant tumors. Non-small cell lung cancer (NSCLC) accounts for more than 3 / 4 of lung cancers. Due to the lack of obvious symptoms of early lung cancer, more than 85% of them are already in the middle and advanced stages when they are diagnosed. Opportunity for surgical treatment. In traditional chemotherapy, anti-cancer drugs used in chemotherapy lack effective selectivity to tumor cells, toxic side effects to normal tissues and drug resistance of tumor cells limit the anti-cancer efficacy and make NSCLC difficult to cure. In order to effectively treat NSCLC, the construction of a nano-tumor targeted delivery system containing anti-tumor drugs to deliver anti-cancer drugs to target tissues, target cells, and even specific or...

Claims

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Application Information

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IPC IPC(8): C07K19/00C07K1/06C07K1/04A61K47/42A61K47/12A61P35/00
CPCA61K47/12A61K47/42C07K14/005C07K19/00C07K2319/09C07K2319/10C12N2710/22022Y02P20/55
Inventor 颜承云谷继伟齐娜廖迎
Owner GUILIN MEDICAL UNIVERSITY