Rapid in-vitro antifungal trichoderma screening method

Abstract

The invention discloses a rapid in-vitro antifungal trichoderma screening method which comprises the following steps: transferring pathogenic bacteria, namely culturing cakes of activated pathogenic bacteria in a 12-pore plate; activating trichoderma strains, namely activating cultured trichoderma till spores are produced, performing confrontation culture, namely inoculating the trichoderma sporesinto the 12-pore plate, and culturing for 4-6 days in a constant-temperature incubator at 28 DEG C; screening antifungal trichoderma strains, namely scanning areas without parasitism of the trichoderma in the pathogenic bacteria in the 12-pore plate by using a bacterial colony area scanner, sequencing the pathogenic bacterium areas from large to small, and judging that the larger the residual area of the pathogenic bacteria is, the poorer the corresponding antifungal resistance of the trichoderma is. By adopting the method, the antifungal trichoderma can be screened with the combination of the porous plate and the bacterial colony area scanner, and screening results of the method are relatively highly related to conventional plate standoff screening results.

Description

technical field [0001] The invention belongs to the field of biological control, in particular to a rapid screening method for antagonizing Trichoderma in vitro. Background technique [0002] Trichoderma is a biocontrol strain that can prevent and control various plant diseases such as Botrytis cinerea, Pythium, Rhizoctonia, Anthracnose, Fusarium, etc. Antibiosis and other biocontrol factors play a role, and at the same time can promote plant growth and improve plant disease resistance. [0003] However, multi-step screening is required to obtain excellent antagonistic Trichoderma. Efficient screening of Trichoderma antagonistic strains is the first and important step in the large-scale development of Trichoderma resources. There are relatively few research reports on the in vitro rapid screening of Trichoderma strains at home and abroad. Most of them follow the traditional method, and the traditional screening method is to initially obtain antagonistic strains through pl...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a rapid screening method for antagonistic Trichoderma in vitro to solve the problem of slow large-scale screening of antagonistic Trichoderma for the low efficiency of traditional antagonistic Trichoderma screening.

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