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Solid-phase micro-extraction determination method for trace aromatic amine in urine

A method of determination, the technology of aromatic amines, applied in the field of analytical chemistry, can solve cumbersome and time-consuming problems, and achieve low detection limit, high sensitivity, and high sensitivity

Active Publication Date: 2018-06-05
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods require pretreatment of urine to remove interferences, which is tedious, time-consuming, and requires the use of large amounts of organic solvents
SPE method can simplify sample processing, but still requires a series of adsorption and desorption processes

Method used

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  • Solid-phase micro-extraction determination method for trace aromatic amine in urine
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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0026] 1. Instruments and reagents

[0027] 1-Naphthylamine, 2-Naphthylamine (purity greater than 98%, Bailingwei Technology Co., Ltd.); Deuterated 2-Naphthylamine (98.8%, Canada CDN Company); Agilent 7890B-7000C Gas Chromatography / Mass Spectrometry, TALBOYS magnetic stirrer; Milli-Q ultrapure water machine (Millipore, USA).

[0028] 2. Preparation of standard working solution

[0029] Prepare standard working solutions (0.01 ng / mL, 0.05 ng / mL, 0.1 ng / mL, 0.15 ng / mL and 0.2 ng / mL) of each aromatic amine in the following 5 concentrations, in which the internal standard concentration is 0.2 ng / mL .

[0030] 3. Sample determination

[0031] The chromatographic conditions used were: the chromatographic column was a polyethylene glycol chromatographic column; the temperature of the injection port was 230-260 °C, the temperature of the quadrupole was 150 °C, the temperature of the ion source was 230 °C; the flow rate of the carrier gas He was 0.8-1.5 mL / min, collision gas N 2...

example 2

[0038] As described in Example 1, another urine product B was selected, and the results are shown in Table 3.

[0039] Table 3 The detection results of each target in sample B

[0040] serial number

example 3

[0042] As described in Example 1, another urine product C was selected, and the results are shown in Table 4.

[0043] Table 4 Detection results of each target in sample C

[0044] serial number

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Abstract

The invention discloses a solid-phase micro-extraction determination method for trace aromatic amine in urine. The method comprises the following step of carrying out qualitative and quantitative analysis on the trace aromatic amine in the urine by adopting a headspace solid-phase micro-extraction and gas chromatography-mass spectrometry / mass spectrometry (GC-MS / MS for short) technology. The method is characterized in that a coating material of solid-phase micro-extraction fibers is a composite material (JUC-Z2 / Gel for short) formed by a covalent organic framework JUC-Z2 and gel; the extraction temperature is 70 to 90 DEG C, the stirring speed is 400 to 600r / min, the extraction time is 30 to 50min, the desorption temperature is 230 to 260 DEG C and the desorption time is 0.5 to 1min. According to the method disclosed by the invention, the solid-phase micro-extraction fibers, which are specially manufactured and are attached with a JUC / Z2-Gel coating, are used for carrying out extraction and concentration pre-treatment on a sample; the method has the technical characteristics of convenience for operation, high sensitivity, good recovery rate and the like.

Description

technical field [0001] The invention relates to a solid-phase microextraction determination method for trace amounts of aromatic amines in urine, belonging to the field of analytical chemistry. Background technique [0002] Aromatic amines are a class of harmful components in cigarette smoke. For example, 1-naphthylamine and 2-naphthylamine are high in mainstream smoke, and are classified as category 3 (carcinogenic to humans) by the International Agency for Cancer (IARC). Not classifiable) and Group 1 (confirmed carcinogen) carcinogen. The determination of the content of aromatic amines in cigarette smoke will be different from the actual feeling of smoking, and there will be large changes due to individual differences. However, direct measurement of aromatic amines in smokers’ urine can be intuitively Evaluate the actual perception of aromatic amines in smokers. Therefore, it is necessary to establish a simple, rapid and accurate method for the determination of aromatic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08G01N30/72G01N30/88
CPCG01N30/02G01N30/06G01N30/08G01N30/72G01N30/88G01N2030/025G01N2030/062G01N2030/884
Inventor 牛佳佳范黎陈连芳李栋李青常马明
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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