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Radioiodine labeling melanin nano-particle, hydrogel, preparation method and application

A nanoparticle and radioactive iodine technology, which is applied in the field of biomedicine, can solve the problems of low labeling rate, poor targeting, and poor label stability, and achieve the effects of improved labeling rate, improved stability, and long residence time.

Active Publication Date: 2018-06-12
JIANGSU INST OF NUCLEAR MEDICINE
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  • Claims
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AI Technical Summary

Problems solved by technology

At present, the main labeling methods of radioactive iodine are chloramine T method, lactoperoxidase method, lodogen iodination method and acylating reagent (Bolton and Hunter reagent) method, wherein, chloramine T method labeling efficiency is high, easy to operate, Good reproducibility, cheap and easy to obtain reagents, is currently the most used iodine labeling method, but this method has disadvantages such as low labeling rate, poor stability of markers, and poor targeting

Method used

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  • Radioiodine labeling melanin nano-particle, hydrogel, preparation method and application
  • Radioiodine labeling melanin nano-particle, hydrogel, preparation method and application
  • Radioiodine labeling melanin nano-particle, hydrogel, preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Dissolve melanin in 0.01N sodium hydroxide solution, stir for 5 minutes, then add 0.01N hydrochloric acid solution to adjust the pH to 6-8, and sonicate at 10W ultrasonic power for 3 minutes to obtain an aqueous solution of melanin nanoparticles, ultrafilter the solution, Wash twice with water to remove impurities, dry to remove water, and obtain melanin nanoparticles with a diameter of 3-6nm;

[0033] After preparing the obtained melanin nanoparticles into a melanin nanoparticle aqueous solution with a concentration of 1 mg / mL, take 1 mL of the melanin nanoparticle aqueous solution and add 20 μL of a silver nitrate solution with a concentration of 10 mg / mL to adjust the pH of the solution to 5-6, and then Stir the reaction at 30°C for 1.5h, remove excess silver ions by ultrafiltration, and the Ag + Link to MNP to get MNP-Ag + ;

[0034] The resulting MNP-Ag+ and Na 123 I was stirred and reacted for 0.5h at a mol ratio of 1:50, and ultrafiltered to obtain MNP-Ag- 12...

Embodiment 2

[0036] Dissolve melanin in 0.1N sodium hydroxide solution, stir for 30 minutes, then add 0.1N hydrochloric acid solution to adjust the pH to 6-8, and sonicate for 1 minute under 20W ultrasonic power to obtain an aqueous solution of melanin nanoparticles, and ultrafilter the solution, Wash 4 times to remove impurities, dry to remove water, and obtain melanin nanoparticles with a diameter of 3-6nm;

[0037] After preparing the obtained melanin nanoparticles into a melanin nanoparticle aqueous solution with a concentration of 1 mg / mL, take 1 mL of the melanin nanoparticle aqueous solution and add 20 μL of a 0.5 mg / mL silver nitrite solution to adjust the pH of the solution to 5-6, Then stir the reaction at 50°C for 0.5h, and remove excess silver ions by ultrafiltration, then the Ag + Link to MNP to get MNP-Ag + ;

[0038] The resulting MNP-Ag + with Na 124 I was stirred and reacted for 1.5h at a mol ratio of 1:0.5, and ultrafiltered to obtain MNP-Ag- 124 I, the marking rate ...

Embodiment 3

[0040] Dissolve melanin in 0.05N sodium hydroxide solution, stir for 10 minutes, then add 0.05N hydrochloric acid solution to adjust the pH to 6-8, and ultrasonicate at 15W ultrasonic power for 2 minutes to obtain an aqueous solution of melanin nanoparticles, ultrafilter the solution, Wash with water 3 times to remove impurities, dry to remove water, and obtain melanin nanoparticles with a diameter of 3-6nm;

[0041]After the obtained melanin nanoparticles are formulated into a melanin nanoparticle aqueous solution with a concentration of 1 mg / mL, 1 mL of the melanin nanoparticle aqueous solution is added to 20 μL of a silver nitrate solution with a concentration of 8.5 mg / mL to adjust the pH of the solution to 5-6, and then Stir the reaction at 40°C for 1 h, and remove excess silver ions by ultrafiltration, then the Ag + Link to MNP to get MNP-Ag + ;

[0042] The resulting MNP-Ag + with Na 131 I presses 1:10 mol ratio stirring reaction 1h, ultrafiltration, obtains MNP-Ag-...

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Abstract

The invention relates to a radioiodine labeling melanin nano-particle, hydrogel, preparation method and application. Melanin nano-particles are combined with metallic silver ions and radioactive iodide ions to obtain the radioiodine labeling melanin nano-particle in a labeling manner, and the radioiodine labeling melanin nano-particles, tetraploid polyethylene glycol and water are mixed and vibrated to obtain the radioiodine labeling melanin nano-particle hydrogel. The labeling rate of the prepared radioiodine labeling melanin nano-particle is not lower than 75%, stability is high, the prepared hydrogel has the advantages that injection, targeted drug deliver and degradation can be achieved, residence time of the radioiodine labeling melanin nano-particle in cancer cells is long, and the radioiodine labeling melanin nano-particle has good treatment effects on cervical cancers, intestinal cancers and prostate cancers.

Description

technical field [0001] The invention relates to a radioactive iodine-labeled melanin nanoparticle, a hydrogel, a preparation method and an application, and belongs to the technical field of biomedicine. Background technique [0002] Tumor is one of the diseases that seriously endanger human health. Now the incidence rate is high. About 6 million people in the world die of malignant tumors every year, and the number of cases is increasing every year. The number of cases in my country is quite large. According to data, it accounts for 10% of the number of cases in the world. 55%. At present, the non-surgical treatment methods for tumors mainly include chemotherapy and radiotherapy, but both methods lack specificity to tumor cells. [0003] The emergence of nanotechnology has brought new hope for the early diagnosis and treatment of tumors. Nanomedicine formed by the combination of nanotechnology and medicine has made breakthroughs in the diagnosis and treatment of diseases, sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/12A61K51/04A61P35/00A61K101/02
CPCA61K51/04A61K51/1213A61K51/1251
Inventor 王辛宇杨敏潘栋辉盛洁杨润琳王立振严骏杰徐宇平赵富宽张波
Owner JIANGSU INST OF NUCLEAR MEDICINE
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