A radioactive iodine-labeled melanin nanoparticle, hydrogel, preparation method, and application
A nanoparticle and radioactive iodine technology, applied in the field of biomedicine, can solve problems such as poor targeting, low labeling rate, and poor stability of markers
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Embodiment 1
[0032] Dissolve melanin in 0.01N sodium hydroxide solution, stir for 5 minutes, then add 0.01N hydrochloric acid solution to adjust the pH to 6-8, and sonicate at 10W ultrasonic power for 3 minutes to obtain an aqueous solution of melanin nanoparticles, ultrafilter the solution, Wash twice with water to remove impurities, dry to remove water, and obtain melanin nanoparticles with a diameter of 3-6nm;
[0033] After preparing the obtained melanin nanoparticles into a melanin nanoparticle aqueous solution with a concentration of 1 mg / mL, add 1 mL of the melanin nanoparticle aqueous solution into 20 μL of a silver nitrate solution with a concentration of 10 mg / mL, adjust the pH of the solution to 5-6, and then Stir the reaction at 30°C for 1.5h, remove excess silver ions by ultrafiltration, and the Ag + Link to MNP to get MNP-Ag + ;
[0034] The resulting MNP-Ag+ and Na 123 I was stirred and reacted for 0.5h at a mol ratio of 1:50, and ultrafiltered to obtain MNP-Ag- 123 I, t...
Embodiment 2
[0036] Dissolve melanin in 0.1N sodium hydroxide solution, stir for 30 minutes, then add 0.1N hydrochloric acid solution to adjust the pH to 6-8, and sonicate at 20W ultrasonic power for 1min to obtain an aqueous solution of melanin nanoparticles, and ultrafilter the solution, Wash 4 times to remove impurities, dry to remove water, and obtain melanin nanoparticles with a diameter of 3-6nm;
[0037] After preparing the obtained melanin nanoparticles into a melanin nanoparticle aqueous solution with a concentration of 1 mg / mL, take 1 mL of the melanin nanoparticle aqueous solution and add 20 μL of a 0.5 mg / mL silver nitrite solution to adjust the pH of the solution to 5-6. Then the reaction was stirred at 50°C for 0.5h, and excess silver ions were removed by ultrafiltration, and the Ag + Link to MNP to get MNP-Ag + ;
[0038] The resulting MNP-Ag + with Na 124 I was stirred and reacted for 1.5h at a mol ratio of 1:0.5, and ultrafiltered to obtain MNP-Ag- 124 I, the marking ...
Embodiment 3
[0040] Dissolve melanin in 0.05N sodium hydroxide solution, stir for 10 minutes, then add 0.05N hydrochloric acid solution to adjust the pH to 6-8, and sonicate at 15W ultrasonic power for 2 minutes to obtain an aqueous solution of melanin nanoparticles, ultrafilter the solution, Wash with water 3 times to remove impurities, dry to remove water, and obtain melanin nanoparticles with a diameter of 3-6nm;
[0041] After the obtained melanin nanoparticles are formulated into a melanin nanoparticle aqueous solution with a concentration of 1 mg / mL, 1 mL of the melanin nanoparticle aqueous solution is added to 20 μL of a silver nitrate solution with a concentration of 8.5 mg / mL to adjust the pH of the solution to 5-6, and then Stir the reaction at 40°C for 1 h, and remove excess silver ions by ultrafiltration, then the Ag + Link to MNP to get MNP-Ag + ;
[0042] The resulting MNP-Ag + with Na 131 I presses 1:10 mol ratio stirring reaction 1h, ultrafiltration, obtains MNP-Ag- 131 ...
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