Fluorescent probe for visual inspection of shooting residues and its preparation method and use
A technology of shooting residues and fluorescent probes, which is applied in the field of chemical analysis of trace evidence in forensic science, achieving the effects of good selectivity, simple synthesis route and low cost
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Embodiment 1
[0034] Example 1 Synthesis and characterization of ratiometric fluorescent probes for measuring shooting residues
[0035] 7-(Diethylamino)-3-formylcoumarin (122 mg, 0.6 mmol) and 2-(3-cyano-4,5,5-trimethylfuran-2-ene) propanedicyanide ( 1g, 0.4mmol) was dissolved in absolute ethanol (5.0mL). NaOH (1-2 mg) was added to the solution at 80°C, followed by stirring for 1 hour. The resulting solid was filtered and washed with cold diethyl ether. The residue was recrystallized from methanol to obtain the desired product as a dark blue solid, namely fluorescent probe I of the present invention, 110.8 mg (65%). 1 H-NMR (300MHz, CDCl 3 ): δ7.94(s,1H, Ar-H),7.92(d,J=15.4Hz,1H,-CH δ =CH-),7.40(d,J=9.6Hz,1H,Ar-H),7.38(d,J=15.4Hz,1H,-CH δ =CH-), 6.70(dd, J=2.6Hz, J=9.6Hz, 1H, Ar-H), 6.51(d, J=2.6Hz, 1H, Ar-H), 3.51(q, J=7.2Hz ,4H,-CH 2 CH 3 ),1.73(s,6H,-CH 3 ),1.28(t, J=7.2Hz,6H,-CH 2 CH 3 ),ppm.MS:[M+1] + = 427.28.
[0036] 3-Formylcoumarin 343 (161 mg, 0.6 mmol) and 2-(3-cya...
Embodiment 2
[0037] Embodiment 2 Fluorescent probe recognition S2- Selective UV-Vis spectra.
[0038] Fluorescent probes I and II were dissolved in PBS buffer (10mmol / L, pH=7.4, 90%DMSO) to prepare a 10 μmol / L solution, and fluorescent probe I had a strong absorption peak at 596nm; fluorescent probe II has a strong absorption peak at 611nm, which is 15nm red-shifted than fluorescent probe I. Add 50mol / L of S to the solution 2- , similar analytes and primer ions (similar analytes include: F - , Cl - , Br - , I - , SCN - , CH 3 COO - ; Primer ion components include: N 3 - , Pb 2+ , ClO 3 - , K + , Ti 3+ , NO 3 - , Ba 2+ ), only by adding S 2- Finally, the absorption peak of fluorescent probe I around 596nm disappeared, and two strong absorption peaks appeared around 342nm and 398nm; the absorption peak of fluorescent probe II around 611nm disappeared, and one near 445nm appeared. Strong absorption peak; and, the color of the solution all changes from dark blue to yellow. ...
Embodiment 3
[0039] Embodiment 3 Fluorescent probe recognition S 2- Selective fluorescence spectra.
[0040] Fluorescent probe I / II was dissolved in PBS buffer (10mmol / L, pH=7.4, 90%DMSO) and was prepared into a solution of 10 μmol / L, and fluorescent probe I had a strong emission peak at 678nm; Needle II has a strong emission peak at 710nm, which is 32nm red-shifted than fluorescent probe I. Add 50 μmol / L S to the solution 2- , similar analytes and primer ions (similar analytes include: F - , Cl - , Br - , I - , SCN - , CH 3 COO - ; Primer ion components include: N 3 - , Pb 2+ , ClO 3 - , K + , Ti 3 + , NO 3 - , Ba 2+ ), only by adding S 2- Finally, the emission peak of fluorescent probe I at 678nm disappeared; a new emission peak appeared at 500nm; the emission peak of fluorescent probe II at 710nm disappeared, and a new emission peak appeared at 511nm; Observation under the irradiation of ultraviolet light shows that the fluorescence color changes from red to light b...
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