A fluorescent sensor for detecting uric acid and its preparation method
A fluorescent sensor and uric acid technology, applied in the direction of fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problems of complex material preparation and complicated detection process, and achieve the effect of rapid detection method
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[0046] A method for preparing a fluorescent sensor for detecting uric acid, comprising the following steps:
[0047] 1) Prepare uric acid solutions with different concentration units;
[0048] 2) The two-dimensional metal-organic framework material NH with fluorescent properties 2 -BDC-MOF nanosheets were dispersed in ultrapure water and ultrasonicated for 30 min, centrifuged to take the supernatant for later use, and the NH in the supernatant 2 -The concentration of BDC-MOF nanosheets is 200 μg / mL;
[0049] 3) Dissolve uricase in tris(hydroxymethyl)aminomethane-hydrochloride buffer solution to prepare uricase solution, and store it in the dark at 4°C for later use;
[0050] 4) Dissolve o-phenylenediamine in tris(hydroxymethyl)aminomethane-hydrochloride buffer solution to prepare o-phenylenediamine solution, and store it in the dark at 4°C for later use;
[0051] 5) Take the 200μg / mL NH prepared in step 2) respectively 2 -50 μL of BDC-MOF nanosheet solution, step 3) prepar...
Embodiment 1
[0073] Example 1 (the method of the present invention detects uric acid)
[0074] 1. First synthesize NH 2 -BDC-MOF nanosheets, the process is as follows:
[0075] 1) Dissolve 1.5 mg of o-aminoterephthalic acid in 3 mL of a mixed solvent of N,N-dimethylformamide and acetonitrile with a volume ratio of 2:1;
[0076] 2) Dissolve 3.0 mg of copper nitrate trihydrate in 3 mL of a mixed solvent of N,N-dimethylformamide and acetonitrile with a volume ratio of 1:2;
[0077] 3) Prepare 2 mL of a mixed solvent of N,N-dimethylformamide and acetonitrile with a volume ratio of 1:1 as a linker;
[0078] 4) The reaction was carried out in a test tube with a diameter of 1.5 cm and 25 mL. The o-aminoterephthalic acid solution was first added to the bottom of the test tube, then 2 mL of linking agent was added, and finally the solution of copper nitrate dissolved in the upper layer was added;
[0079] 5) In a 40°C water bath, heat the o-aminoterephthalic acid layer and react for 24 hours;
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