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A qualitative and quantitative detection method for paraquat based on dried blood spot samples

A quantitative detection method and dry blood spot technology, which is applied in the field of qualitative and quantitative detection of paraquat based on dried blood spot samples, can solve the problems of long operation time, low recovery rate, large sample size, etc., and achieve short operation time and high detection efficiency. The effect of low limit and easy re-examination

Active Publication Date: 2021-01-12
HEBEI MEDICAL UNIVERSITY
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Problems solved by technology

[0003] Aiming at the technical problems of large sample size, complicated operation, low recovery rate, long operation time and high detection limit of the pretreatment method for paraquat poisoned blood samples in the prior art, the present invention provides a method based on dried blood spots Qualitative and quantitative detection method of paraquat in samples

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  • A qualitative and quantitative detection method for paraquat based on dried blood spot samples
  • A qualitative and quantitative detection method for paraquat based on dried blood spot samples
  • A qualitative and quantitative detection method for paraquat based on dried blood spot samples

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Embodiment 1

[0030]The embodiment of the present invention provides a qualitative and quantitative detection method for paraquat based on dried blood spot samples, which is specifically as follows:

[0031]1. Preparation of dried blood spot sample: Take 100μL of whole blood and drop it onOn the FTA blood collection card (Whatman, USA), after the blood is completely infiltrated, it is placed in a 1000-1200W microwave for 4-6 minutes, sealed and packaged and stored at room temperature.

[0032]2. Sample pretreatment: use 8mm punch to preparePunch holes on the FTA blood card, put the blood spot in a 2mL centrifuge tube, add 200μL of mobile phase and 5μl of 4μg / mL internal standard ethyl paraquat, then immediately ultrasound for 10 minutes, and then centrifuge at 12000r / min for 5min at 4℃ , Take the supernatant and filter it with a 0.2μm filter membrane for testing.

[0033]3. Preparation of standard curve: add 5μL of 0.2μg / mL–200μg / mL paraquat standard solution to 1mL blank blood to prepare a series of conc...

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Abstract

The invention relates to a qualitative and quantitative detection method for paraquat based on a dried blood spot sample, which is characterized in that the sample is pretreated by a dried blood spot method and detected by a high-performance liquid chromatography / mass spectrometry analysis method. The present invention does not require a large amount of blood collection, less invasiveness, no centrifugation of blood, short operation time, less interference from blood-borne viruses such as HIV, hepatitis B and hepatitis C virus, low detection limit, and dry Blood spots allow samples to be transported and stored at room temperature for retesting at sites other than where they were sampled or at other times.

Description

Technical field[0001]The present invention relates to the technical field of biological detection, and in particular to a method for qualitative and quantitative detection of paraquat based on dried blood spot samples.Background technique[0002]Paraquat is a highly toxic compound to humans and animals. It is the herbicide with the highest mortality rate of acute poisoning in humans. Paraquat poisoning leads to high morbidity and mortality, and there is no specific medicine. The mortality rate of oral poisoning can reach 90 %the above. With the increase in cases of herbicide poisoning such as paraquat, the qualitative and quantitative detection of such poisons is of great significance. A reliable and efficient sample pretreatment method is the first step of the entire detection process, and it is also the most time-consuming part, accounting for about 80% of the entire detection process. At present, the methods used for the pretreatment of paraquat poisoning blood samples are mainly p...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 文迪马春玲杨杨向平于峰
Owner HEBEI MEDICAL UNIVERSITY